CN105561372A - Preparation method of chitosan gelatin-based electrostatic spinning composite hydrogel wound dressing - Google Patents
Preparation method of chitosan gelatin-based electrostatic spinning composite hydrogel wound dressing Download PDFInfo
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- CN105561372A CN105561372A CN201510952349.XA CN201510952349A CN105561372A CN 105561372 A CN105561372 A CN 105561372A CN 201510952349 A CN201510952349 A CN 201510952349A CN 105561372 A CN105561372 A CN 105561372A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/28—Polysaccharides or their derivatives
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/32—Proteins, polypeptides; Degradation products or derivatives thereof, e.g. albumin, collagen, fibrin, gelatin
- A61L15/325—Collagen
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/44—Medicaments
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/48—Surfactants
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0024—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid beta-D-Glucans; (beta-1,3)-D-Glucans, e.g. paramylon, coriolan, sclerotan, pachyman, callose, scleroglucan, schizophyllan, laminaran, lentinan or curdlan; (beta-1,6)-D-Glucans, e.g. pustulan; (beta-1,4)-D-Glucans; (beta-1,3)(beta-1,4)-D-Glucans, e.g. lichenan; Derivatives thereof
- C08B37/0027—2-Acetamido-2-deoxy-beta-glucans; Derivatives thereof
- C08B37/003—Chitin, i.e. 2-acetamido-2-deoxy-(beta-1,4)-D-glucan or N-acetyl-beta-1,4-D-glucosamine; Chitosan, i.e. deacetylated product of chitin or (beta-1,4)-D-glucosamine; Derivatives thereof
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- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
- D04H1/40—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties
- D04H1/42—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties characterised by the use of certain kinds of fibres insofar as this use has no preponderant influence on the consolidation of the fleece
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- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
- D04H1/70—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres
- D04H1/72—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged
- D04H1/728—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged by electro-spinning
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/23—Carbohydrates
- A61L2300/232—Monosaccharides, disaccharides, polysaccharides, lipopolysaccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/252—Polypeptides, proteins, e.g. glycoproteins, lipoproteins, cytokines
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
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- D—TEXTILES; PAPER
- D10—INDEXING SCHEME ASSOCIATED WITH SUBLASSES OF SECTION D, RELATING TO TEXTILES
- D10B—INDEXING SCHEME ASSOCIATED WITH SUBLASSES OF SECTION D, RELATING TO TEXTILES
- D10B2509/00—Medical; Hygiene
- D10B2509/02—Bandages, dressings or absorbent pads
- D10B2509/022—Wound dressings
Abstract
The invention discloses a preparation method of a chitosan gelatin-based electrostatic spinning composite hydrogel wound dressing, belonging to the field of dressing preparation. The method comprises the following steps: preparing a spinning solution from carboxymethyl chitosan and gelatin as raw materials, and performing electrostatic spinning to obtain a fiber film dressing; and performing a crosslinking reaction with glutaraldehyde steam to obtain the dressing. The composite hydrogel prepared by the preparation method is soft, has good elasticity, can absorb a large amount of wound exudate to keep a wound wet, and also accelerates the healing and tissue repair of the wound; and moreover, fewer wastes are generated in comparison with a traditional gauze dressing, and environmental pressure is reduced.
Description
Technical field
The invention discloses a kind of preparation method of chitosan gelatin-based Static Spinning composite aquogel wound dressing, belong to dressing and manufacture field.
Background technology
Medical dressing is a kind of disposable medical treatment and nursing articles for use, and the dressing after using is a kind of clinical waste with high risk, easily causes cross infection and environmental pollution, needs to focus on.China produces a large amount of dressing refuses every year, causes huge pressure to work for the treatment of and environmental conservation, how to carry out to this hazardous medical waste the great attention that minimizing has caused people.
Traditional gauze dressing exists cannot keep wound surface moistening, and wound healing postpones; Usually stick together with wound, during replacing, easily cause wound dehiscence, damage cambium on the one hand, be unfavorable for wound healing; When dressing is soaked into, pathogen is easily passed through, and causes wound infection; Liquid absorption is little, and each use amount is large, and the wasting of resources is large, the problem that the refuse amount of generation is many.Aerogel dressing is a kind of novel moist medical dressing, obtains develop rapidly in recent years.The main component of this dressing is the high-molecular gel with tridimensional network, and in dressing, hydrogel mainly plays the effect absorbing wound fluid, keep moistening, the debridement of wound surface and accelerating wound.The outstanding water retention property of hydrogel can make wound surface keep moistening, can need not change dressings for several days, minimizing more obvious than gauze dressing in the materials'use amount of each wound.Not only be conducive to improving therapeutic effect, alleviate the misery of patient, be more conducive to reducing refuse generation, thus alleviate the pressure to environment.
Chitosan has good biological activity, biocompatibility, biological degradability, it can promote wound healing and tissue repair, the concern of the extremely people of the application in novel wound dressing and organizational project, recent years, the research about this respect was a lot, but also there is many deficiencies.Fibroblast plays an important role in wound healing and tissue repair, about Chitosan-phospholipid complex to the research of its growth effect system and complete not enough.
Summary of the invention
The technical problem that the present invention mainly solves: exist for current gauze dressing and cannot keep wound surface moistening, wound healing postpones, easily wound dehiscence is caused during replacing, pathogen is easily passed through, cause the problem of wound infection, provide and be mixed into chitosan and gelatin the preparation method that aerogel dressing made by base material, the carboxymethyl chitosan that the present invention obtains and gelatin are that raw material obtains spinning liquid and to carry out after electrostatic spinning obtaining fibrous membrane dressing, carry out cross-linking reaction with glutaraldehyde steam again and obtain dressing, the composite aquogel that the present invention prepares has softness, good springiness, a large amount of wound fluid can be absorbed, keep wound surface moistening, and the healing of accelerated in wounds and tissue repair, and it is fewer than the waste material of normal gauze dressing generation, alleviate the pressure to environment.
In order to solve the problems of the technologies described above, the technical solution adopted in the present invention is:
(1) taking 20 ~ 30g chitosan, to be dissolved in 200 ~ 300mL mass concentration be in the sodium hydroxide solution of 50%, after stirring with Glass rod, move in the there-necked flask with agitator, Dropping funnel and thermometer, 150 ~ 200mL isopropyl alcohol is dripped by Dropping funnel, start agitator and add 10 ~ 15g monoxone 3 ~ 5 times with after the rotating speed of 200 ~ 300r/min stirring 10 ~ 20min point, continue stirring 20 ~ 30min;
(2) mixed liquor is put into buchner funnel, with absolute ethanol washing sucking filtration 2 ~ 3 times, separation obtains white powder, getting the white powder that 20 ~ 25g obtains is dissolved in 200 ~ 250mL distilled water, dropwise add 10 ~ 20mL acetone until produce without precipitation, obtain carboxymethyl chitosan with after vacuum desiccator drying;
(3) get 50 ~ 100g gelatin and put into conical flask, add 300 ~ 400mL distilled water, to be placed on shaking table after oscillating reactions 10 ~ 20min, be placed on magnetic stirrer, slowly stir with the rotating speed of 100 ~ 200r/min, add the above-mentioned obtained carboxymethyl chitosan of 15 ~ 20g while stirring, continue to stir until it dissolves completely;
(4) move in water-bath by conical flask, be heated in 60 ~ 70 DEG C of backward bottles and drip the silver nitrate solution that concentration is 0.01mol/L, dripping quantity is 1 ~ 3% of gelatin gross mass, dropwises rear insulation reaction 2 ~ 3h and obtains mixed liquor;
(5) above-mentioned mixed liquor is put into sonic oscillation instrument, with Dropping funnel be in the calcium alginate dropwise instillation mixed liquor of 30% by 20 ~ 25mL mass concentration, control rate of addition to make to dropwise in its 10 ~ 15min, start sonic oscillation instrument afterwards and obtain blend spinning liquid with the power ultrasonic of 200 ~ 300W vibration 30 ~ 40min;
(6) above-mentioned obtained spinning liquid is put into high-voltage electrostatic spinning device hopper, start HV generator, spinning liquid infusion pump is extruded and is sprayed by spinning nozzle again, the spinning liquid jet of ejection is cured formation nanofiber, be arranged on collecting board with unordered shape, form the fibrous membrane dressing of similar nonwoven cloth-like;
(7) take out after irradiating 2 ~ 3h under obtained fibrous membrane dressing being placed in uviol lamp, lie on the rustless steel filter of exsiccator, with evaporating dish splendid attire 10 ~ 20mL glutaraldehyde bottom exsiccator, being heated to 110 ~ 120 DEG C makes glutaraldehyde evaporate formation steam, glutaraldehyde steam is utilized to obtain aerogel dressing to fibrous membrane dressing cross-linking reaction 1 ~ 2h, finally take out dressing, be placed in thermostatic drying chamber, after removing remaining glutaraldehyde with the temperature drying 24 ~ 36h of 45 ~ 55 DEG C, obtain a kind of chitosan gelatin-based Static Spinning composite aquogel wound dressing.
Described spinning jet curing be the aqueous solution that is made into for 5:1 in mass ratio using sulphuric acid and sodium sulfate as coagulating bath, spinning jet is solidified.
Application process of the present invention: the composite aquogel dressing that the present invention obtains may be used for various surgical operation, the postoperative wound of plastic operation or wound surface, first with sterile gauze, wound surface surrounding skin is dried before using, select the aerogel dressing flap coverage of 3 ~ 5cm larger than wound surface again, wrap up with sterile gauze more afterwards, dressing and wound surface are close to, replacing construction is determined according to wound fluid, transudate is many, dressing absorbs saturated should replacing in time, transudate is few, can changing every 1 ~ 3 day, as being used in the privileged sites such as face, corresponding mode can be taked to be fixed.
The invention has the beneficial effects as follows:
(1) composite aquogel that the present invention prepares has softness, good springiness, can absorb a large amount of wound fluid, keeps wound surface moistening, can the healing of accelerated in wounds and tissue repair;
(2) waste material that the composite aquogel that the present invention prepares produces than normal gauze dressing is few, alleviates the pressure to environment.
Detailed description of the invention
First taking 20 ~ 30g chitosan, to be dissolved in 200 ~ 300mL mass concentration be in the sodium hydroxide solution of 50%, after stirring with Glass rod, move in the there-necked flask with agitator, Dropping funnel and thermometer, 150 ~ 200mL isopropyl alcohol is dripped by Dropping funnel, start agitator and add 10 ~ 15g monoxone 3 ~ 5 times with after the rotating speed of 200 ~ 300r/min stirring 10 ~ 20min point, continue stirring 20 ~ 30min; Mixed liquor is put into buchner funnel, with absolute ethanol washing sucking filtration 2 ~ 3 times, be separated and obtain white powder, get the white powder that 20 ~ 25g obtains and be dissolved in 200 ~ 250mL distilled water, dropwise add 10 ~ 20mL acetone until produce without precipitation, obtain carboxymethyl chitosan with after vacuum desiccator drying; Get 50 ~ 100g gelatin and put into conical flask, add 300 ~ 400mL distilled water, to be placed on shaking table after oscillating reactions 10 ~ 20min, be placed on magnetic stirrer, slowly stir with the rotating speed of 100 ~ 200r/min, add the carboxymethyl chitosan that 15 ~ 20g is obtained while stirring, continue to stir until it dissolves completely; Conical flask moved in water-bath, be heated in 60 ~ 70 DEG C of backward bottles and drip the silver nitrate solution that concentration is 0.01mol/L, dripping quantity is 1 ~ 3% of gelatin gross mass, dropwises rear insulation reaction 2 ~ 3h and obtains mixed liquor; Mixed liquor is put into sonic oscillation instrument, with Dropping funnel be in the calcium alginate dropwise instillation mixed liquor of 30% by 20 ~ 25mL mass concentration, control rate of addition to make to dropwise in its 10 ~ 15min, start sonic oscillation instrument afterwards and obtain blend spinning liquid with the power ultrasonic of 200 ~ 300W vibration 30 ~ 40min; Obtained spinning liquid is put into high-voltage electrostatic spinning device hopper, start HV generator, spinning liquid infusion pump is extruded and is sprayed by spinning nozzle again, the spinning liquid jet of ejection is cured formation nanofiber, be arranged on collecting board with unordered shape, form the fibrous membrane dressing of similar nonwoven cloth-like; Take out after irradiating 2 ~ 3h under obtained fibrous membrane dressing being placed in uviol lamp, lie on the rustless steel filter of exsiccator, with evaporating dish splendid attire 10 ~ 20mL glutaraldehyde bottom exsiccator, being heated to 110 ~ 120 DEG C makes glutaraldehyde evaporate formation steam, glutaraldehyde steam is utilized to obtain aerogel dressing to fibrous membrane dressing cross-linking reaction 1 ~ 2h, finally take out dressing, be placed in thermostatic drying chamber, after removing remaining glutaraldehyde with the temperature drying 24 ~ 36h of 45 ~ 55 DEG C, obtain a kind of chitosan gelatin-based Static Spinning composite aquogel wound dressing.
Described spinning jet curing be the aqueous solution that is made into for 5:1 in mass ratio using sulphuric acid and sodium sulfate as coagulating bath, spinning jet is solidified.
First taking 20g chitosan, to be dissolved in 200mL mass concentration be in the sodium hydroxide solution of 50%, after stirring with Glass rod, move in the there-necked flask with agitator, Dropping funnel and thermometer, 150mL isopropyl alcohol is dripped by Dropping funnel, start agitator and add 10g monoxone 3 times with after the rotating speed of 200r/min stirring 10min point, continue to stir 20min; Mixed liquor is put into buchner funnel, with absolute ethanol washing sucking filtration 2 ~ 3 times, be separated and obtain white powder, get the white powder that 20g obtains and be dissolved in 200mL distilled water, dropwise add 10mL acetone until produce without precipitation, obtain carboxymethyl chitosan with after vacuum desiccator drying; Get 50g gelatin and put into conical flask, add 300mL distilled water, to be placed on shaking table after oscillating reactions 10min, be placed on magnetic stirrer, slowly stir with the rotating speed of 100r/min, add the carboxymethyl chitosan that 15g is obtained while stirring, continue to stir until it dissolves completely; Conical flask moved in water-bath, be heated in 60 DEG C of backward bottles and drip the silver nitrate solution that concentration is 0.01mol/L, dripping quantity is 1% of gelatin gross mass, dropwises rear insulation reaction 2h and obtains mixed liquor; Mixed liquor is put into sonic oscillation instrument, with Dropping funnel be in the calcium alginate dropwise instillation mixed liquor of 30% by 20mL mass concentration, control rate of addition to make to dropwise in its 10min, start sonic oscillation instrument afterwards and obtain blend spinning liquid with the power ultrasonic of 200W vibration 30min; Obtained spinning liquid is put into high-voltage electrostatic spinning device hopper, start HV generator, spinning liquid infusion pump is extruded and is sprayed by spinning nozzle again, the spinning liquid jet of ejection is cured formation nanofiber, be arranged on collecting board with unordered shape, form the fibrous membrane dressing of similar nonwoven cloth-like; Take out after irradiating 2h under obtained fibrous membrane dressing is placed in uviol lamp, lie on the rustless steel filter of exsiccator, with evaporating dish splendid attire 10mL glutaraldehyde bottom exsiccator, being heated to 110 DEG C makes glutaraldehyde evaporate formation steam, glutaraldehyde steam is utilized to obtain aerogel dressing to fibrous membrane dressing cross-linking reaction 1h, finally take out dressing, be placed in thermostatic drying chamber, after removing remaining glutaraldehyde with the dry 24h of the temperature of 45 DEG C, obtain a kind of chitosan gelatin-based Static Spinning composite aquogel wound dressing.
Described spinning jet curing be the aqueous solution that is made into for 5:1 in mass ratio using sulphuric acid and sodium sulfate as coagulating bath, spinning jet is solidified.
The composite aquogel dressing that the present invention obtains may be used for various surgical operation, the postoperative wound of plastic operation or wound surface, first with sterile gauze, wound surface surrounding skin is dried before using, select the aerogel dressing flap coverage of 3cm larger than wound surface again, wrap up with sterile gauze more afterwards, dressing and wound surface are close to, replacing construction is determined according to wound fluid, transudate is many, dressing absorbs saturated should replacing in time, transudate is few, can changing every 1 day, as being used in the privileged sites such as face, corresponding mode can be taked to be fixed.
First taking 25g chitosan, to be dissolved in 250mL mass concentration be in the sodium hydroxide solution of 50%, after stirring with Glass rod, move in the there-necked flask with agitator, Dropping funnel and thermometer, 175mL isopropyl alcohol is dripped by Dropping funnel, start agitator and add 13g monoxone 4 times with after the rotating speed of 250r/min stirring 15min point, continue to stir 25min; Mixed liquor is put into buchner funnel, with absolute ethanol washing sucking filtration 2 times, be separated and obtain white powder, get the white powder that 23g obtains and be dissolved in 225mL distilled water, dropwise add 15mL acetone until produce without precipitation, obtain carboxymethyl chitosan with after vacuum desiccator drying; Get 80g gelatin and put into conical flask, add 350mL distilled water, to be placed on shaking table after oscillating reactions 15min, be placed on magnetic stirrer, slowly stir with the rotating speed of 150r/min, add the carboxymethyl chitosan that 17g is obtained while stirring, continue to stir until it dissolves completely; Conical flask moved in water-bath, be heated in 65 DEG C of backward bottles and drip the silver nitrate solution that concentration is 0.01mol/L, dripping quantity is 2% of gelatin gross mass, dropwises rear insulation reaction 2.5h and obtains mixed liquor; Mixed liquor is put into sonic oscillation instrument, with Dropping funnel be in the calcium alginate dropwise instillation mixed liquor of 30% by 23mL mass concentration, control rate of addition to make to dropwise in its 13min, start sonic oscillation instrument afterwards and obtain blend spinning liquid with the power ultrasonic of 250W vibration 35min; Obtained spinning liquid is put into high-voltage electrostatic spinning device hopper, start HV generator, spinning liquid infusion pump is extruded and is sprayed by spinning nozzle again, the spinning liquid jet of ejection is cured formation nanofiber, be arranged on collecting board with unordered shape, form the fibrous membrane dressing of similar nonwoven cloth-like; Take out after irradiating 2.5h under obtained fibrous membrane dressing is placed in uviol lamp, lie on the rustless steel filter of exsiccator, with evaporating dish splendid attire 15mL glutaraldehyde bottom exsiccator, being heated to 115 DEG C makes glutaraldehyde evaporate formation steam, glutaraldehyde steam is utilized to obtain aerogel dressing to fibrous membrane dressing cross-linking reaction 1.5h, finally take out dressing, be placed in thermostatic drying chamber, after removing remaining glutaraldehyde with the dry 28h of the temperature of 50 DEG C, obtain a kind of chitosan gelatin-based Static Spinning composite aquogel wound dressing.
Described spinning jet curing be the aqueous solution that is made into for 5:1 in mass ratio using sulphuric acid and sodium sulfate as coagulating bath, spinning jet is solidified.
The composite aquogel dressing that the present invention obtains may be used for various surgical operation, the postoperative wound of plastic operation or wound surface, first with sterile gauze, wound surface surrounding skin is dried before using, select the aerogel dressing flap coverage of 4cm larger than wound surface again, wrap up with sterile gauze more afterwards, dressing and wound surface are close to, replacing construction is determined according to wound fluid, transudate is many, dressing absorbs saturated should replacing in time, transudate is few, can changing every 2 days, as being used in the privileged sites such as face, corresponding mode can be taked to be fixed.
First taking 30g chitosan, to be dissolved in 300mL mass concentration be in the sodium hydroxide solution of 50%, after stirring with Glass rod, move in the there-necked flask with agitator, Dropping funnel and thermometer, 200mL isopropyl alcohol is dripped by Dropping funnel, start agitator and add 15g monoxone 5 times with after the rotating speed of 300r/min stirring 20min point, continue to stir 30min; Mixed liquor is put into buchner funnel, with absolute ethanol washing sucking filtration 3 times, be separated and obtain white powder, get the white powder that 25g obtains and be dissolved in 250mL distilled water, dropwise add 20mL acetone until produce without precipitation, obtain carboxymethyl chitosan with after vacuum desiccator drying; Get 100g gelatin and put into conical flask, add 400mL distilled water, to be placed on shaking table after oscillating reactions 20min, be placed on magnetic stirrer, slowly stir with the rotating speed of 200r/min, add the carboxymethyl chitosan that 20g is obtained while stirring, continue to stir until it dissolves completely; Conical flask moved in water-bath, be heated in 70 DEG C of backward bottles and drip the silver nitrate solution that concentration is 0.01mol/L, dripping quantity is 3% of gelatin gross mass, dropwises rear insulation reaction 3h and obtains mixed liquor; Mixed liquor is put into sonic oscillation instrument, with Dropping funnel be in the calcium alginate dropwise instillation mixed liquor of 30% by 25mL mass concentration, control rate of addition to make to dropwise in its 15min, start sonic oscillation instrument afterwards and obtain blend spinning liquid with the power ultrasonic of 300W vibration 40min; Obtained spinning liquid is put into high-voltage electrostatic spinning device hopper, start HV generator, spinning liquid infusion pump is extruded and is sprayed by spinning nozzle again, the spinning liquid jet of ejection is cured formation nanofiber, be arranged on collecting board with unordered shape, form the fibrous membrane dressing of similar nonwoven cloth-like; Take out after irradiating 3h under obtained fibrous membrane dressing is placed in uviol lamp, lie on the rustless steel filter of exsiccator, with evaporating dish splendid attire 20mL glutaraldehyde bottom exsiccator, being heated to 120 DEG C makes glutaraldehyde evaporate formation steam, glutaraldehyde steam is utilized to obtain aerogel dressing to fibrous membrane dressing cross-linking reaction 2h, finally take out dressing, be placed in thermostatic drying chamber, after removing remaining glutaraldehyde with the dry 36h of the temperature of 55 DEG C, obtain a kind of chitosan gelatin-based Static Spinning composite aquogel wound dressing.
Described spinning jet curing be the aqueous solution that is made into for 5:1 in mass ratio using sulphuric acid and sodium sulfate as coagulating bath, spinning jet is solidified.
The composite aquogel dressing that the present invention obtains may be used for various surgical operation, the postoperative wound of plastic operation or wound surface, first with sterile gauze, wound surface surrounding skin is dried before using, select the aerogel dressing flap coverage of 5cm larger than wound surface again, wrap up with sterile gauze more afterwards, dressing and wound surface are close to, replacing construction is determined according to wound fluid, transudate is many, dressing absorbs saturated should replacing in time, transudate is few, can changing every 2 days, as being used in the privileged sites such as face, corresponding mode can be taked to be fixed.
Claims (2)
1. a preparation method for chitosan gelatin-based Static Spinning composite aquogel wound dressing, is characterized in that concrete preparation process is:
(1) taking 20 ~ 30g chitosan, to be dissolved in 200 ~ 300mL mass concentration be in the sodium hydroxide solution of 50%, after stirring with Glass rod, move in the there-necked flask with agitator, Dropping funnel and thermometer, 150 ~ 200mL isopropyl alcohol is dripped by Dropping funnel, start agitator and add 10 ~ 15g monoxone 3 ~ 5 times with after the rotating speed of 200 ~ 300r/min stirring 10 ~ 20min point, continue stirring 20 ~ 30min;
(2) mixed liquor is put into buchner funnel, with absolute ethanol washing sucking filtration 2 ~ 3 times, separation obtains white powder, getting the white powder that 20 ~ 25g obtains is dissolved in 200 ~ 250mL distilled water, dropwise add 10 ~ 20mL acetone until produce without precipitation, obtain carboxymethyl chitosan with after vacuum desiccator drying;
(3) get 50 ~ 100g gelatin and put into conical flask, add 300 ~ 400mL distilled water, to be placed on shaking table after oscillating reactions 10 ~ 20min, be placed on magnetic stirrer, slowly stir with the rotating speed of 100 ~ 200r/min, add the above-mentioned obtained carboxymethyl chitosan of 15 ~ 20g while stirring, continue to stir until it dissolves completely;
(4) move in water-bath by conical flask, be heated in 60 ~ 70 DEG C of backward bottles and drip the silver nitrate solution that concentration is 0.01mol/L, dripping quantity is 1 ~ 3% of gelatin gross mass, dropwises rear insulation reaction 2 ~ 3h and obtains mixed liquor;
(5) above-mentioned mixed liquor is put into sonic oscillation instrument, with Dropping funnel be in the calcium alginate dropwise instillation mixed liquor of 30% by 20 ~ 25mL mass concentration, control rate of addition to make to dropwise in its 10 ~ 15min, start sonic oscillation instrument afterwards and obtain blend spinning liquid with the power ultrasonic of 200 ~ 300W vibration 30 ~ 40min;
(6) above-mentioned obtained spinning liquid is put into high-voltage electrostatic spinning device hopper, start HV generator, spinning liquid infusion pump is extruded and is sprayed by spinning nozzle again, the spinning liquid jet of ejection is cured formation nanofiber, be arranged on collecting board with unordered shape, form the fibrous membrane dressing of similar nonwoven cloth-like;
(7) take out after irradiating 2 ~ 3h under obtained fibrous membrane dressing being placed in uviol lamp, lie on the rustless steel filter of exsiccator, with evaporating dish splendid attire 10 ~ 20mL glutaraldehyde bottom exsiccator, being heated to 110 ~ 120 DEG C makes glutaraldehyde evaporate formation steam, glutaraldehyde steam is utilized to obtain aerogel dressing to fibrous membrane dressing cross-linking reaction 1 ~ 2h, finally take out dressing, be placed in thermostatic drying chamber, after removing remaining glutaraldehyde with the temperature drying 24 ~ 36h of 45 ~ 55 DEG C, obtain a kind of chitosan gelatin-based Static Spinning composite aquogel wound dressing.
2. the preparation method of a kind of Static Spinning lignin/cellulose acetate Micron-nano composites according to claim 1, it is characterized in that: described spinning jet curing be the aqueous solution that is made into for 5:1 in mass ratio using sulphuric acid and sodium sulfate as coagulating bath, spinning jet is solidified.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106310361A (en) * | 2016-11-02 | 2017-01-11 | 常州市鼎日环保科技有限公司 | Preparation method for hydrocolloid dressing for promoting wound repairing |
| CN106758217A (en) * | 2016-12-31 | 2017-05-31 | 武汉工程大学 | A kind of crosslinking preparation method of gelatin/chitosan composite nano-fiber membrane |
| CN108633879A (en) * | 2018-04-20 | 2018-10-12 | 袁杰 | A kind of circulating water sterilizing agent |
| CN109646706A (en) * | 2018-12-28 | 2019-04-19 | 上海纳米技术及应用国家工程研究中心有限公司 | Method for preparing anti-scar overlay film using method of electrostatic spinning and products thereof and application |
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| CN112760811A (en) * | 2020-12-30 | 2021-05-07 | 中国热带农业科学院南亚热带作物研究所 | Chitosan-alginate composite nanofiber membrane and preparation method and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101096424A (en) * | 2007-06-27 | 2008-01-02 | 东华大学 | Glutin nano fabric film containing nano silver and preparation and application thereof |
| CN101596325A (en) * | 2009-07-08 | 2009-12-09 | 中国人民解放军第三军医大学野战外科研究所 | Nano-silver biological sponge and preparation method thereof |
| CN104562438A (en) * | 2013-10-17 | 2015-04-29 | 中国科学院理化技术研究所 | Gelatin-based micro-nanofiber membrane material, preparation method and utilization thereof |
-
2015
- 2015-12-20 CN CN201510952349.XA patent/CN105561372A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101096424A (en) * | 2007-06-27 | 2008-01-02 | 东华大学 | Glutin nano fabric film containing nano silver and preparation and application thereof |
| CN101596325A (en) * | 2009-07-08 | 2009-12-09 | 中国人民解放军第三军医大学野战外科研究所 | Nano-silver biological sponge and preparation method thereof |
| CN104562438A (en) * | 2013-10-17 | 2015-04-29 | 中国科学院理化技术研究所 | Gelatin-based micro-nanofiber membrane material, preparation method and utilization thereof |
Non-Patent Citations (6)
| Title |
|---|
| JUNG-JHIH CHANG ET AL: "Electrospun anti-adhesion barrier made of chitosan alginate for reducing peritoneal adhesions", 《CARBOHYDRATE POLYMERS》 * |
| SUNGCHEAL MOON ET AL: "Electrospinning of Heated Gelatin-Sodium Alginate-Water Solutions", 《POLYMER ENGINEERING AND SCIENCE》 * |
| XUPIN ZHUANG ET AL: "Electrospun chitosan/gelatin nanofibers containing silver nanoparticles", 《CARBOHYDRATE POLYMERS》 * |
| 秦益民: "《功能性医用敷料 第2版》", 31 March 2014, 中国纺织出版社 * |
| 肖长发等: "《化学纤维概论 第3版》", 30 June 2015, 中国纺织出版社 * |
| 马滢等: "羧甲基壳聚糖的制备及其在抗菌纸中的应用", 《功能材料》 * |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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| CN106310361B (en) * | 2016-11-02 | 2019-04-26 | 青岛华元精细生物制品有限公司 | A kind of preparation method for promoting wound to repair bearing hydrocolloid dressing |
| CN106758217A (en) * | 2016-12-31 | 2017-05-31 | 武汉工程大学 | A kind of crosslinking preparation method of gelatin/chitosan composite nano-fiber membrane |
| CN108633879A (en) * | 2018-04-20 | 2018-10-12 | 袁杰 | A kind of circulating water sterilizing agent |
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| CN109771690A (en) * | 2019-01-10 | 2019-05-21 | 广东工业大学 | A kind of nanofiber wound dressing and edible package and preparation method thereof |
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Application publication date: 20160511 |