CN109646706A - Method for preparing anti-scar overlay film using method of electrostatic spinning and products thereof and application - Google Patents
Method for preparing anti-scar overlay film using method of electrostatic spinning and products thereof and application Download PDFInfo
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- CN109646706A CN109646706A CN201811621688.XA CN201811621688A CN109646706A CN 109646706 A CN109646706 A CN 109646706A CN 201811621688 A CN201811621688 A CN 201811621688A CN 109646706 A CN109646706 A CN 109646706A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/62—Compostable, hydrosoluble or hydrodegradable materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/225—Mixtures of macromolecular compounds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/44—Medicaments
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
- D01D5/0015—Electro-spinning characterised by the initial state of the material
- D01D5/003—Electro-spinning characterised by the initial state of the material the material being a polymer solution or dispersion
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F1/00—General methods for the manufacture of artificial filaments or the like
- D01F1/02—Addition of substances to the spinning solution or to the melt
- D01F1/10—Other agents for modifying properties
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F8/00—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof
- D01F8/02—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof from cellulose, cellulose derivatives, or proteins
-
- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
- D04H1/70—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres
- D04H1/72—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged
- D04H1/728—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged by electro-spinning
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/23—Carbohydrates
- A61L2300/232—Monosaccharides, disaccharides, polysaccharides, lipopolysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/12—Nanosized materials, e.g. nanofibres, nanoparticles, nanowires, nanotubes; Nanostructured surfaces
Abstract
The present invention relates to a kind of methods for preparing anti-scar overlay film using method of electrostatic spinning and products thereof and application, to there is the drug for inhibiting cell fibrotic lesions to combine with electrostatic spinning technique, obtain the overlay film for being able to suppress larynx tracheae postoperative scar and skin trauma scar, the preparation of spinning solution first, then carboxymethyl chitosan is added in spinning solution with the concentration of 5-10mg/ml, the preparation of the last anti-scar overlay film of method of electrostatic spinning.The present invention prepares nano-fiber film using method of electrostatic spinning, has good flexibility and tensile strength, is outstanding covering material；Drug can be sustained by nanofiber in the present invention, can obtain ideal therapeutic effect in prevention scar.
Description
Technical field
The present invention relates to a kind of methods for preparing anti-scar overlay film using method of electrostatic spinning and products thereof and application, specially
To there is the drug for inhibiting cell fibrotic lesions to combine with electrostatic spinning technique, obtain being able to suppress the postoperative scar of larynx tracheae
The overlay film of trace, skin surface scar.The invention belongs to field of medical biology.
Background technique
Nanotechnology in terms of creating the new material with advanced application have huge potentiality, and nanofiber then due to
Its fibre diameter is less than 100nm, so that surface area with higher and porosity, possess the incomparable excellent spy of general fibre
Property.Electrostatic spinning technique (Electrospinning) is a kind of spinning for preparing superfine fibre as tractive force by electrostatic force
Technology is knitted, the spun fibre diameter minimum of this technology energy can be to the superfine fibre of 1nm.Electrostatic spinning process has many exclusive
Characteristic, the material that it is used is very extensive, and many polymer melts or solution all can be used.
Cicatricial Laryngotracheal stenosis is trachea cannula, laryngosurgery operation common complication.Although disease incidence is not high, treatment
Extremely difficult, operative treatment can not obtain ideal effect due to its traumatic and higher restenosis rate, and drug therapy
Then there is positive effect in early prevention tracheostenosis.Cutaneous scar tissue be in human body exocuticle processes of wound repair must
Right product, and cicatrix of skin can be divided into hyperplastic scar, keloid, epigenetic-surface reworking, atrophic scar, the new scar of contracture,
Depressed scar, scar cancer.The formation of scar is mainly more with burn, wound, operation, infection, foreign matter, heredity and immune function etc.
Kind factor is related.The wherein function that hyperplastic scar, keloid seriously damage the objective looks of patient, influence part, and with
Itch pain, influence quality of life, and so far, is difficult to have a kind of exact method or product to prevent and treat scar.
Carboxymethyl chitosan (Carboxymethyl Chitosan) is a kind of polysaccharide, by chitosan through carboxy methylation and
, carboxymethyl has strongly hydrophilic, makes it have better biological degradability and histocompatbility, there is hemostasis, analgesic and inhibition
The effect of microorganism growth.Furthermore it can promote epithelial cell growth, inhibit Fb proliferation and migration, reach the shape for inhibiting cicatricial tissue
At and effect, to accelerate wound healing.
Patent name is a kind of " T-type trachea cannula and its preparation method containing mitomycin with anti-scar function " Patent No.
Anti- scar drug is applied to intubation surface by CN201810058757, the middle method using conjugate coupling, certain is prevented although having
Scar effect is controlled, but has easy scratch, the deficiency of slow release effect difference can not play good prevention and treatment cicatrix of trachea effect.
In the present invention, we joined carboxymethyl chitosan as anti-scar using electrostatic spinning technique in spinning material
Trace drug has made medical overlay film, for preventing and treating larynx trachea cannula, the scar sexual trauma that is formed in laryngosurgery operation.
It not only has good pharmacological properties, also more compatible with living tissue.This overlay film has outstanding answer in medical domain
Use prospect.
Summary of the invention
In view of the deficiencies of the prior art, anti-scar overlay film is prepared using method of electrostatic spinning it is an object of that present invention to provide a kind of
Method.Specially will there is the drug for inhibiting cell fibrotic lesions to combine with electrostatic spinning technique, is able to suppress
The overlay film of larynx tracheae postoperative scar, skin surface scar.
Another object of the present invention is: providing a kind of anti-scar coated products of above method preparation.
Another object of the present invention is to: a kind of application of the said goods is provided.
The object of the invention is realized by following proposal: a method of anti-scar overlay film being prepared using method of electrostatic spinning, is wrapped
Include following steps:
(1) preparation of spinning solution
Using the spinning solution of fibroin albumen/gelatin production nanofiber, fibroin albumen/gelatin is pressed 75/25 respectively, 50/50,
25/75 mass ratio is dissolved in hexafluoroisopropanol respectively, is configured to fibroin albumen/gelatin mixed solution that mass fraction is 4%,
The spinning solution with suitable physical properties is obtained by adjusting the mass ratio of fibroin albumen and gelatin；
(2) carboxymethyl chitosan is added in the spinning solution that step (1) obtains with the concentration of 5-10mg/ml, magnetic agitation
To being completely dissolved；
(3) preparation of the anti-scar overlay film of method of electrostatic spinning
It takes 5ml needle tubing to suck above-mentioned spinning solution, is placed in electrostatic spinning apparatus fixture；The positive voltage of electrostatic spinning syringe needle is arranged
For 11kV, receive plate negative voltage and be set as -1kV, spinning flow velocity is set as 0.01ml/min, and receiving plate distance is 15cm's
Under the conditions of, it prepares electrospun fibers film and places it in spare in vacuum oven, obtain anti-scar overlay film.
Wherein, fibroin albumen/gelatin is pressed 100/0,75/25,50/50,25/75,0/100 respectively in the step (1)
Mass ratio be dissolved in hexafluoroisopropanol respectively, be configured to mass fraction be 4% fibroin albumen/gelatin mixed solution, be placed in glass
In glass bottle magnetic agitation 12 hours or more at room temperature after sealing, until solution is uniformly mixed.
Fibroin albumen/gelatin mass ratio most preferably 25 to 75.
Carboxymethyl chitosan is prepared as in the step (2), and 20g chitosan is suspended in 200mL aqueous isopropanol,
It is added with stirring 50.4 mL of NaOH of 10mol/L, is divided into 6 parts of additions, every part of interval 20min；Add rear lye mixture after
Continuous stirring 45min, adds solid monoxone 24.0g, is divided into 5 parts, every 5min is added 1 part；60 DEG C of reaction 3h are heated to, so
Afterwards, be 7.0 with glacial acetic acid tune pH into reaction mixture plus 17 mL of Q water, filtering, solid product with 70% methanol aqueous solution
300mL is rinsed, and can be substituted with ethanol solution；It is rinsed again with anhydrous methanol, sample product obtained by drying at 60 DEG C.
The present invention provides a kind of anti-scar overlay film, is prepared according to any of the above-described the method.
The present invention provide a kind of anti-scar overlay film inhibit larynx tracheae postoperative scar, the application in skin surface scar.
The present invention constructs a kind of electrostatic spinning overlay film with anti-scar function of novel load medicine, for preventing and treating larynx
Cicatricial caused by when trachea cannula, laryngosurgery operation damages, skin surface scar proliferation.The film of this method preparation has good
Good flexibility, tensile strength, degradation rate be fast, permeable breathable and adjustable dissolubility, and has controlled drug release.
By carrying out surface topography test, Mechanics Performance Testing, expansion rate survey to different proportion fibroin albumen/gelatin fiber
Examination, degradation rate test, Cell growth ability/Adhesion property/competence for added value/growthform test, obtain the fibroin of suitable proportion
Albumen/gelatine nano fiber auxiliary material ratio is 25/75, i.e., the mass ratio of fibroin fiber albumen and gelatin is 25 to 75, this ratio
The film obtained under example by electrostatic spinning has the following characteristics that
(1) consistency, it is soft, it can be cut with the shape of intubation and equipment；
(2) good gas permeability guarantees the transmission of moisture and air, and keeps preferably wet degree；
(3) high-liquid absorbing rate can effectively improve the absorptivity of wound tissue's liquid；
(4) degradable, and catabolite has no toxic side effect, it is possible to reduce the pain of patient.
The present invention has the advantages that
(1) present invention prepares nano-fiber film using method of electrostatic spinning, has good flexibility and tensile strength, is excellent
Elegant covering material.
(2) present invention can be by adjusting high molecular material used in electrostatic spinning, to adjust its dissolubility and permeable
Gas and other physical characteristics can highly be adapted to the environment of human body.
(3) drug can be sustained by nanofiber in the present invention, can be obtained in prevention scar and preferably be controlled
Therapeutic effect.
Detailed description of the invention
Fig. 1 is the scanning electron microscope sem picture of anti-scar film.
Specific embodiment
Below by way of specific embodiment, the technical scheme of the present invention will be further described.Embodiment below is to this
The further explanation of invention, and do not limit the scope of the invention.
Carboxymethyl chitosan used in the embodiment of the present invention, is prepared according to the following steps:
20g chitosan is suspended in 200mL aqueous isopropanol, 50.4 mL of NaOH of 10mol/L is added with stirring, is divided into 6
Part is added, every part of interval 20min；It adds rear lye mixture to continue to stir 45min, adds solid monoxone 24.0g, be divided into
5 parts, every 5min is added 1 part；60 DEG C of reaction 3h are heated to, then, 17 mL of Q water are added into reaction mixture, with glacial acetic acid tune pH
It is 7.0, filtering, solid product is rinsed with 70% methanol aqueous solution 300mL, can be substituted with ethanol solution；It is rushed again with anhydrous methanol
It washes, sample product carboxymethyl chitosan obtained by drying at 60 DEG C.
Embodiment 1
It is a kind of to prepare anti-scar overlay film using method of electrostatic spinning, it prepares as follows:
(1) preparation of spinning solution
Using fibroin albumen/gelatin production nanofiber spinning solution, the mass ratio by adjusting fibroin albumen and gelatin is obtained
Spinning solution with suitable physical properties.Fibroin albumen/gelatin is dissolved in hexafluoroisopropanol by 100/0 mass ratio, is matched
It is set to fibroin albumen/gelatin mixed solution that mass fraction is 4%, is placed in vial after sealing magnetic agitation 12 at room temperature
Hour or more, until solution is uniformly mixed.
(2) carboxymethyl chitosan is added in the spinning solution that step (1) obtains with the concentration of 5mg/ml, magnetic agitation
To being completely dissolved；
(3) preparation of the anti-scar overlay film of method of electrostatic spinning takes 5ml needle tubing to suck above-mentioned spinning solution, is placed in electrostatic spinning apparatus folder
In tool.The positive voltage of electrostatic spinning syringe needle is set as 11kV, receives plate negative voltage and is set as -1kV, spinning flow velocity is set as
0.01ml/min receives plate distance to prepare electrospun fibers film and placing it in vacuum drying under conditions of 15cm
Spare in case, the scanning electron microscope sem picture of obtained anti-scar film is shown in Fig. 1.
Embodiment 2
It is a kind of to prepare anti-scar overlay film using method of electrostatic spinning, it prepares as follows:
(1) preparation of spinning solution
Using fibroin albumen/gelatin production nanofiber spinning solution, the mass ratio by adjusting fibroin albumen and gelatin is obtained
Spinning solution with suitable physical properties.Fibroin albumen/gelatin is dissolved in hexafluoroisopropanol by 175/25 mass ratio, is matched
It is set to fibroin albumen/gelatin mixed solution that mass fraction is 4%, is placed in vial after sealing magnetic agitation 12 at room temperature
Hour or more, until solution is uniformly mixed.
(2) carboxymethyl chitosan is added in the spinning solution that step (1) obtains with the concentration of 7mg/ml, magnetic agitation
To being completely dissolved；
(3) preparation of the anti-scar overlay film of method of electrostatic spinning
It takes 5ml needle tubing to suck above-mentioned spinning solution, is placed in electrostatic spinning apparatus fixture.The positive voltage of electrostatic spinning syringe needle is arranged
For 11kV, receive plate negative voltage and be set as -1kV, spinning flow velocity is set as 0.01ml/min, and receiving plate distance is 15cm's
Under the conditions of, it prepares electrospun fibers film and places it in spare in vacuum oven.
Embodiment 3
It is a kind of to prepare anti-scar overlay film using method of electrostatic spinning, it prepares as follows:
(1) preparation of spinning solution
Using fibroin albumen/gelatin production nanofiber spinning solution, the mass ratio by adjusting fibroin albumen and gelatin is obtained
Spinning solution with suitable physical properties.Fibroin albumen/gelatin is dissolved in hexafluoroisopropanol by 25/75 mass ratio, is matched
It is set to fibroin albumen/gelatin mixed solution that mass fraction is 4%, is placed in vial after sealing magnetic agitation 12 at room temperature
Hour or more, until solution is uniformly mixed.
(2) carboxymethyl chitosan is added in the spinning solution that step (1) obtains with the concentration of 10mg/ml, magnetic force stirs
It mixes to being completely dissolved；
(3) preparation of the anti-scar overlay film of method of electrostatic spinning takes 5ml needle tubing to suck above-mentioned spinning solution, is placed in electrostatic spinning apparatus folder
In tool.The positive voltage of electrostatic spinning syringe needle is set as 11kV, receives plate negative voltage and is set as -1kV, spinning flow velocity is set as
0.01ml/min receives plate distance to prepare electrospun fibers film and placing it in vacuum drying under conditions of 15cm
It is spare in case.
Claims (6)
1. a kind of method for preparing anti-scar overlay film using method of electrostatic spinning, it is characterised in that include the following steps:
(1) preparation of spinning solution
Using the spinning solution of fibroin albumen/gelatin production nanofiber, fibroin albumen/gelatin is pressed 75/25 respectively, 50/50,
25/75 mass ratio is dissolved in hexafluoroisopropanol respectively, is configured to fibroin albumen/gelatin mixed solution that mass fraction is 4%,
The spinning solution with suitable physical properties is obtained by adjusting the mass ratio of fibroin albumen and gelatin；
(2) carboxymethyl chitosan is added in the spinning solution that step (1) obtains with the concentration of 5-10mg/ml, magnetic agitation
To being completely dissolved；
(3) preparation of the anti-scar overlay film of method of electrostatic spinning
It takes 5ml needle tubing to suck above-mentioned spinning solution, is placed in electrostatic spinning apparatus fixture；The positive voltage of electrostatic spinning syringe needle is arranged
For 11kV, receive plate negative voltage and be set as -1kV, spinning flow velocity is set as 0.01ml/min, and receiving plate distance is 15cm's
Under the conditions of, it prepares electrospun fibers film and places it in spare in vacuum oven, obtain anti-scar overlay film.
2. the method for preparing anti-scar overlay film using method of electrostatic spinning according to claim 1, it is characterised in that: the step
(1) in fibroin albumen/gelatin is dissolved in hexafluoroisopropanol by 75/25,50/50,25/75 mass ratio respectively respectively, is configured
Fibroin albumen/gelatin the mixed solution for being 4% at mass fraction, is placed in vial that magnetic agitation 12 is small at room temperature after sealing
When more than, until solution be uniformly mixed.
3. the method for preparing anti-scar overlay film using method of electrostatic spinning according to claim 2, it is characterised in that: fibroin egg
The mass ratio of white/gelatin most preferably 25 to 75.
4. the method for preparing anti-scar overlay film using method of electrostatic spinning according to claim 1, which is characterized in that the step
(2) carboxymethyl chitosan is prepared according to the following steps in: 20g chitosan being suspended in 200mL aqueous isopropanol, is added with stirring
50.4 mL of NaOH of 10mol/L is divided into 6 parts of additions, every part of interval 20min；Rear lye mixture is added to continue to stir
45min adds solid monoxone 24.0g, is divided into 5 parts, every 5min is added 1 part；60 DEG C of reaction 3h are heated to, then, to anti-
It answers and adds 17 mL of Q water in mixture, be 7.0 with glacial acetic acid tune pH, filtering, solid product is rushed with 70% methanol aqueous solution 300mL
It washes, can be substituted with ethanol solution；It is rinsed again with anhydrous methanol, sample is dried at 60 DEG C and obtained.
5. a kind of anti-scar overlay film, it is characterised in that -4 any the methods are prepared according to claim 1.
6. a kind of scar overlay film anti-according to claim 5 answering in inhibition larynx tracheae postoperative scar, skin surface scar
With.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114272444A (en) * | 2021-12-06 | 2022-04-05 | 盐城工学院 | Medicine-carrying absorbable anti-adhesion barrier and preparation method thereof |
| CN114540977A (en) * | 2022-02-19 | 2022-05-27 | 河南工程学院 | Tussah silk fibroin composite nanofiber for wound repair |
| CN115721768A (en) * | 2022-12-01 | 2023-03-03 | 国纳之星(上海)纳米科技发展有限公司 | Preparation method of anti-inflammatory silk fibroin film, product and application thereof |
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