CN107737364A - A kind of wound dressing and preparation method thereof - Google Patents
A kind of wound dressing and preparation method thereof Download PDFInfo
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- CN107737364A CN107737364A CN201711187390.8A CN201711187390A CN107737364A CN 107737364 A CN107737364 A CN 107737364A CN 201711187390 A CN201711187390 A CN 201711187390A CN 107737364 A CN107737364 A CN 107737364A
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- electrospinning
- polycaprolactone
- wound dressing
- fibrinogen
- liquid
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/32—Proteins, polypeptides; Degradation products or derivatives thereof, e.g. albumin, collagen, fibrin, gelatin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/26—Macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/44—Medicaments
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/22—Lipids, fatty acids, e.g. prostaglandins, oils, fats, waxes
- A61L2300/222—Steroids, e.g. corticosteroids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
- A61L2300/406—Antibiotics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
- A61L2300/414—Growth factors
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/60—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a special physical form
- A61L2300/602—Type of release, e.g. controlled, sustained, slow
Abstract
The invention discloses a kind of wound dressing, and comprising medicine and pharmaceutical carrier, pharmaceutical carrier is nano fibrous membrane, and the preparing raw material of nano fibrous membrane includes fibrinogen and polycaprolactone；It is prepared with the following method：A) electrospinning liquid is prepared:Fibrinogen is dissolved in the mixed solvent, is prepared into 85 125mg/ml fibrin stoste；Polycaprolactone is dissolved in mass concentration is made in organic solvent as 12% 26% solution；Then the fibrin stoste of preparation is well mixed with polycaprolactone solution, obtains fibrinogen/polycaprolactone mixing electrospinning liquid；B) electrospinning:By step a fibrinogen/polycaprolactone mix electrospinning liquid with electrospinning device in the 25kv of applied voltage 15, electrospinning flow velocity be 3 7ml/h, carry out electrospinning under conditions of to receive distance be 10 20cm, rotating speed is 400 600r/min, obtain nano fibrous membrane；C) the nano fibrous membrane dry sterilization that step b) is prepared.The wound dressing of the present invention has good biocompatibility, can prevent the intrusion of bacterium, accelerate the healing of wound.
Description
Technical field
The present invention relates to technical field of biological materials, and in particular to a kind of wound dressing and its system based on fibrinogen
Preparation Method.
Background technology
Wound dressing is a kind of important medical dressing for being used to cover wound, can play temporary barrier to impaired skin
Protective effect, the infection of wound is controlled or avoided, an environment for being advantageous to quickly heal is provided wound.Former, as Asia
Fiber crops, honey, animal tallow etc. are all once used as wound dressing materials, and with the continuous development of science and technology, people are to wound dressing
Requirement improve constantly, traditional dressing can not meet to require.At present, the research of wound dressing is conceived to living using biology more
Property material.Bioactive materials broadly refer to the material that can promote cytoactive or new regeneration.Except continually developing
Outside new material, some advanced technologies also gradually introduce the production process of dressing, fine for synthesising biological medical function
Dimension.
Fibrinogen is a plasma glycoprotein for participating in coagulation process later stage.In coagulation process, fiber egg
White original hydrolysis transformation fibroblast cells in the presence of fibrin ferment, ultimately form blood clot.Fibrinogen is not only involved in blood coagulation
Function, it has played important function in processes of wound repair, gradually causes the concern of people in recent years.And fibrin is original
Source is also quite varied, can not only be obtained from itself, and can be obtained from mammal.
Polycaprolactone has good mechanical property, histocompatbility, controllable biological degradability and easy processing formability
It is widely used in field of pharmaceutical biology.But it lacks bioactivity, by the fibrinogen of remaining bioactivity
An effective approach of the compound structure with good biological activity, the wound dressing of suitable mechanical intensity.
There is nano fibrous membrane prepared by electrostatic spinning unique structure it is shown many brand-new functional characteristics,
These characteristics show wide application prospect in many fields, wherein most strikingly biomedical sector should
With.Wound dressing prepared by electrostatic spinning has enough spaces, it can be ensured that its liquid and gas exchanges with the external world, together
When can prevent the intrusion of bacterium again.There is good adhesiveness to the moist surface of a wound simultaneously, and larger specific surface area is advantageous to liquid
The suction of body and medicine locally discharge on skin, and then cause these materials to be suitable for surface of a wound closure hemostasis.
The content of the invention
A kind of preparation method of novel wound dressing is provided it is an object of the invention to overcome above mentioned problem, it prepares skill
Art cost simple to operate is cheap, and environmental pollution is small, is suitable for industrialized production；And resulting nano fibrous membrane mechanical performance
It is good, higher porosity and specific surface area.
To achieve the above object, the technical scheme is that a kind of wound dressing, the wound dressing include medicine and
Pharmaceutical carrier, the pharmaceutical carrier are nano fibrous membrane, and the preparing raw material of the nano fibrous membrane includes fibrinogen and gathered
Caprolactone.
Preferably, the nano fibrous membrane is prepared with the following method：
A) electrospinning liquid is prepared:Fibrinogen is dissolved in the mixed solvent, is prepared into 85-125mg/ml fibrinogen
Liquid；Polycaprolactone is dissolved in the solution for being made that mass concentration is 12%-26% in organic solvent；Then by the fibrin of preparation
Stoste is well mixed with polycaprolactone solution, obtains fibrinogen/polycaprolactone mixing electrospinning liquid；
B) electrospinning:Step a fibrinogen/polycaprolactone is mixed into electrospinning liquid electrospinning device in applied voltage
Electricity is carried out under conditions of it is 10-20cm that 15-25kv, electrospinning flow velocity, which be 3-7ml/h, receives distance, rotating speed is 400-600r/min
Spin, obtain nano fibrous membrane；
C) the nano fibrous membrane dry sterilization that step b) is prepared.
Preferably, mixed solvent is that volume ratio is 9 in the step a)：1 organic solvent liquid mixing compatible with water；
The organic solvent of the in the mixed solvent is hexafluoroisopropanol；
The aqueous phase solution of the in the mixed solvent is distilled water, MEM minimal mediums, physiological saline, phosphate buffer
At least one of.
Preferably, the organic solvent of the step a) is hexafluoroisopropanol, chloroform, dimethylformamide, tetrahydrochysene furan
At least one of mutter with trifluoroethanol.
Preferably, the medicine is penicillins, dexamethasone, EGF, TGF, blood vessel endothelium
At least one of Porcine HGF, nerve growth factor.
A kind of preparation method of wound dressing, comprises the following steps：
A) electrospinning liquid is prepared:Fibrinogen is dissolved in the mixed solvent, it is 85-125mg/ml's to be prepared into mass fraction
Fibrin stoste；Polycaprolactone is dissolved in the solution for being made that mass fraction is 12%-26% in organic solvent；Then will prepare
Fibrin stoste be well mixed with polycaprolactone solution, obtain fibrinogen/polycaprolactone mixing electrospinning liquid；And institute
State in fibrinogen/polycaprolactone mixing electrospinning liquid and add medicine, fibrinogen/polycaprolactone/medicine mixing electricity is made
Spin liquid；
B) electrospinning:Step a) fibrinogen/polycaprolactone/medicine is mixed into electrospinning liquid with electrospinning device outside
Under conditions of it is 10-20cm that making alive 15-25kv, electrospinning flow velocity, which be 3-7ml/h, receives distance, rotating speed is 400-600r/min
Electrospinning is carried out, obtains wound dressing；
C) the wound dressing dry sterilization for obtaining step b).
Preferably, mixed solvent is that volume ratio is 9 in the step a)：1 organic solvent liquid mixing compatible with water；
The organic solvent of the in the mixed solvent is hexafluoroisopropanol；
The aqueous phase solution of the in the mixed solvent is distilled water, MEM minimal mediums, physiological saline, phosphate buffer
At least one of.
Preferably, the organic solvent of the step a) is hexafluoroisopropanol, chloroform, dimethylformamide, tetrahydrochysene furan
At least one of mutter with trifluoroethanol.
Preferably, the medicine is penicillins, dexamethasone, EGF, TGF, blood vessel endothelium
At least one of Porcine HGF, nerve growth factor.
Preferably, wound dressing is is dried in vacuo by the dry sterilization in the step c), then with Co irradiation or ultraviolet
Sterilizing.
Beneficial effects of the present invention：
1st, the wound dressing prepared by the present invention has the nanofibrous structures similar with extracellular matrix, and with good
Biocompatibility, the features such as higher specific surface area, porosity, be advantageous to extraneous liquid and gas exchanges, while again
The intrusion of bacterium can be prevented, accelerates the healing of wound.
2nd, the macroshape of wound dressing prepared by the present invention depends primarily on the shape selection different shape of receiver (such as
Tubulose, rectangle, circle etc.) receiver, can obtain having variously-shaped nano fibrous membrane with direct convenience.
3rd, wound dressing prepared by the present invention has good mechanical performance, it can be ensured that not easily broken using process use
Split.
4th, preparation condition of the invention is gentle, and medicine is dissolved in into electrospinning liquid carries out electrospinning, is that medicaments uniformity is distributed to fibre
Tie up in film, can not only avoid the denaturation of active component from failing, influence the structure of nano fibrous membrane by changing preparation condition, also
The purpose of Drug controlled release can be reached.
Embodiment
To better illustrate the object, technical solutions and advantages of the present invention, below in conjunction with specific embodiment to the present invention
It is described further.
Embodiment 1
A kind of wound dressing, comprising medicine and pharmaceutical carrier, pharmaceutical carrier is nano fibrous membrane, the preparation of nano fibrous membrane
Raw material includes fibrinogen and polycaprolactone.
The preparation method of the wound dressing, comprises the following steps；
A) electrospinning liquid is prepared:By fibrinogen 5ml hexafluoroisopropanols：MEM minimal medium=9：1 mixed solvent
In, magnetic agitation 30 minutes under the conditions of 37 DEG C, obtained 100mg/ml fibrin stoste；It is different that polycaprolactone is dissolved in hexafluoro
In propyl alcohol, the solution that mass fraction is 12% is configured to, magnetic agitation 30 minutes under the conditions of 37 DEG C, it is molten that polycaprolactone is made
Liquid；Then by above-mentioned made fibrin stoste and polycaprolactone by volume 3:1 mixing, and EGF is added, mix
Close uniform, obtained electrospinning liquid.
B) electrospinning:Step a fibrinogen/polycaprolactone is mixed into electrospinning liquid electrospinning device, voltage is set
For 19kv, electrospinning speed is 4ml/h, rotating speed 400r/min, and it is 20cm to receive distance, and the general 90cm of area is obtained after 3 hours2
Nano fibrous membrane.
C) nano fibrous membrane that step b) is prepared is dried in vacuo 6h under normal temperature, sealed with lucifuge every wet bag, spoke
4 DEG C of preservations are placed in after penetrating sterilizing.
Embodiment 2
The preparation method of the wound dressing of the present embodiment, comprises the following steps；
A) electrospinning liquid is prepared:Fibrinogen is dissolved in 5ml hexafluoroisopropanols：Physiological saline=9：1 in the mixed solvent,
Magnetic agitation 30 minutes under the conditions of 37 DEG C, 85mg/ml fibrin stoste is made；Polycaprolactone is dissolved in chloroform
In, the solution that mass fraction is 14% is configured to, magnetic agitation 30 minutes under the conditions of 37 DEG C, polycaprolactone solution is made；So
Afterwards by above-mentioned made fibrin stoste and polycaprolactone by volume 2:1 mixing, and penicillins are added, it is well mixed, system
Obtain electrospinning liquid.
B) electrospinning:Step a fibrinogen/polycaprolactone is mixed into electrospinning liquid electrospinning device, voltage is set
For 15kv, electrospinning speed is 3ml/h, rotating speed 450r/min, and it is 15cm to receive distance, and the general 90cm of area is obtained after 3 hours2
Nano fibrous membrane.
C) nano fibrous membrane that step b) is prepared is dried in vacuo 6h under normal temperature, sealed with lucifuge every wet bag, spoke
4 DEG C of preservations are placed in after penetrating sterilizing.
Embodiment 3
The preparation method of the wound dressing of the present embodiment, comprises the following steps；
A) electrospinning liquid is prepared:Fibrinogen is dissolved in hexafluoroisopropanol：Phosphate buffer=9：1 mixed solvent
In, magnetic agitation 30 minutes under the conditions of 37 DEG C, obtained 90mg/ml fibrin stoste；Polycaprolactone is dissolved in dimethyl
In formamide, the solution that mass fraction is 16% is configured to, magnetic agitation 30 minutes under the conditions of 37 DEG C, it is molten that polycaprolactone is made
Liquid；Then by above-mentioned made fibrin stoste and polycaprolactone by volume 1:1 mixing, and dexamethasone is added, mixing is equal
It is even, electrospinning liquid is made.
B) electrospinning:Step a fibrinogen/polycaprolactone is mixed into electrospinning liquid electrospinning device, voltage is set
For 17kv, electrospinning speed is 5ml/h, rotating speed 500r/min, and it is 16cm to receive distance, and the general 90cm of area is obtained after 3 hours2
Nano fibrous membrane.
C) nano fibrous membrane that step b) is prepared is dried in vacuo 6h under normal temperature, sealed with lucifuge every wet bag, spoke
4 DEG C of preservations are placed in after penetrating sterilizing.
Embodiment 4
The preparation method of the wound dressing of the present embodiment, comprises the following steps；
A) electrospinning liquid is prepared:Fibrinogen is dissolved in hexafluoroisopropanol：Distilled water=9：1 in the mixed solvent, 37
Magnetic agitation 30 minutes under the conditions of DEG C, 95mg/ml fibrin stoste is made；Polycaprolactone is dissolved in dimethylformamide
In, the solution that mass fraction is 20% is configured to, magnetic agitation 30 minutes under the conditions of 37 DEG C, polycaprolactone solution is made；So
Afterwards by above-mentioned made fibrin stoste and polycaprolactone by volume 1:2 mixing, and TGF is added, mixing is equal
It is even, electrospinning liquid is made.
B) electrospinning:Step a fibrinogen/polycaprolactone is mixed into electrospinning liquid electrospinning device, voltage is set
For 20kv, electrospinning speed is 5ml/h, rotating speed 550r/min, and it is 10cm to receive distance, and the general 90cm of area is obtained after 3 hours2
Nano fibrous membrane.
C) nano fibrous membrane that step b) is prepared is dried in vacuo 6h under normal temperature, sealed with lucifuge every wet bag, spoke
4 DEG C of preservations are placed in after penetrating sterilizing.
Embodiment 5
The preparation method of the wound dressing of the present embodiment, comprises the following steps；
A) electrospinning liquid is prepared:Fibrinogen is dissolved in hexafluoroisopropanol：Phosphate buffer=9：1 mixed solvent
In, magnetic agitation 30 minutes under the conditions of 37 DEG C, obtained 110mg/ml fibrin stoste；Polycaprolactone is dissolved in tetrahydrochysene furan
In muttering, the solution that mass fraction is 24% is configured to, magnetic agitation 30 minutes under the conditions of 37 DEG C, polycaprolactone solution is made；
Then by above-mentioned made fibrin stoste and polycaprolactone by volume 1:3 mixing, and add vascular endothelial cell growth because
Son, it is well mixed, electrospinning liquid is made.
B) electrospinning:Step a fibrinogen/polycaprolactone is mixed into electrospinning liquid electrospinning device, voltage is set
For 22kv, electrospinning speed is 6ml/h, rotating speed 500r/min, and it is 18cm to receive distance, and the general 90cm of area is obtained after 3 hours2
Nano fibrous membrane.
C) nano fibrous membrane that step b) is prepared is dried in vacuo 6h under normal temperature, sealed with lucifuge every wet bag, spoke
4 DEG C of preservations are placed in after penetrating sterilizing.
Embodiment 6
The preparation method of the wound dressing of the present embodiment, comprises the following steps；
A) electrospinning liquid is prepared:Fibrinogen is dissolved in hexafluoroisopropanol：Phosphate buffer=9：1 mixed solvent
In, magnetic agitation 30 minutes under the conditions of 37 DEG C, obtained 125mg/ml fibrin stoste；Polycaprolactone is dissolved in trifluoro second
In alcohol, the solution that mass fraction is 26% is configured to, magnetic agitation 30 minutes under the conditions of 37 DEG C, polycaprolactone solution is made；
Then by above-mentioned made fibrin stoste and polycaprolactone by volume 1:4 mixing, and nerve growth factor is added, mixing is equal
It is even, electrospinning liquid is made.
B) electrospinning:Step a fibrinogen/polycaprolactone is mixed into electrospinning liquid electrospinning device, voltage is set
For 25kv, electrospinning speed is 7ml/h, rotating speed 600r/min, and it is 20cm to receive distance, and the general 90cm of area is obtained after 3 hours2
Nano fibrous membrane.
C) nano fibrous membrane that step b) is prepared is dried in vacuo 6h under normal temperature, sealed with lucifuge every wet bag, spoke
4 DEG C of preservations are placed in after penetrating sterilizing.
Control group 1
The wound dressing preparation method is：0.5g fibrinogens are weighed, are dissolved in 5ml hexafluoroisopropanols：MEM is cultivated substantially
Base=9：1 in the mixed solvent, magnetic agitation 30 minutes under the conditions of 37 DEG C, obtained 100mg/ml fibrin stoste；And
EGF is added, is well mixed, electrospinning liquid is made.
Electrospinning liquid obtained above is subjected to electrostatic spinning, setting voltage is 19kv, and electrospinning speed is 4ml/h, and rotating speed is
400r/min, it is 20cm to receive distance, and the general 90cm of area is obtained after 3 hours2Nano fibrous membrane.
The film is dried in vacuo 6h under normal temperature, sealed with lucifuge every wet bag, 4 DEG C of preservations are placed in after radiation sterilization.
Control group 2
The wound dressing preparation method is：Polycaprolactone is dissolved in hexafluoroisopropanol, is configured to mass fraction as 12%
Solution, magnetic agitation 30 minutes under the conditions of 37 DEG C, polycaprolactone solution is made；And EGF is added, mixing is equal
It is even, electrospinning liquid is made.
Electrospinning liquid obtained above is subjected to electrostatic spinning, setting voltage is 19kv, and electrospinning speed is 4ml/h, and rotating speed is
400r/min, it is 20cm to receive distance, and the general 90cm2 of area nano fibrous membrane is obtained after 3 hours.
The film is dried in vacuo 6h under normal temperature, sealed with lucifuge every wet bag, 4 DEG C of preservations are placed in after radiation sterilization.
The sign of the obtained wound dressing of the present invention and control group
(1) fibrillarin stoste and polycaprolactone nanofiber electrospinning film and control group prepared by embodiment 1~6 is measured respectively
Tensile strength, elongation at break and the porosity of 1~2 electrospinning film, measurement result are shown in Table 1.
Table 1 is tensile strength, elongation at break, the characterization result of porosity
As can be seen from Table 1, nanofiber electrospinning film of the invention has good tensile strength, elongation at break, with
And higher porosity, so as to be advantageous to liquid and gas exchanges with the external world, while the intrusion of bacterium can be prevented again, accelerate wound
The healing of mouth, and with the increase of polycaprolactone content, the increase of its mechanical performance.
(2) fibrillarin stoste and the polycaprolactone nanofiber electrospinning membrane fiber for measuring the preparation of embodiment 1~6 respectively are straight
Footpath, as a result as shown in table 2.
Table 2 shows that the diameter of electrospinning film is less than the diameter of general cell, therefore electrospinning film can be while gas permeability be ensured
Prevent cambium from growing into, so as to avoid surface of a wound adhesion from occurring.
(3) animal experiments in vivo observes the effect that electrospinning film of the present invention accelerates wound healing as feature wound dressing
A, after the nano-cellulose film for preparing embodiment 3 soaks 10 minutes in 70% ethanol solution, then physiology salt is used
Water soaks 30 minutes, with the purpose for reaching sterilization and thoroughly removing the solvent composition of possible residual in nano-cellulose film；
The skin of back wound repair that invention wound dressing is respectively used to SD rats (cuts off a diameter of 1cm circle b,
Shape full thickness skin), Continuous Observation wound healing situation.Compared with traditional gauze dressing, created using the feature of the present invention
The wound for hindering dressing generally restored in the 18th day, treatment phase interval dressing in 2 days 1 time, and used normal gauze class dressing
Wound could substantially restore in the 28th day, dressing every other day 1 time between treatment phase.Meanwhile feature wound dressing of the invention and wound
Face is less to stick together, and is not easy bleeding during dressing, and the dressing of normal gauze class often with surface of a wound adhesion, bleeding.
(4) biocompatibility of invention wound dressing
A, after wound dressings prepared by embodiment 1~6 are soaked 10 minutes in 70% ethanol solution, then delayed with phosphate
Fliud flushing (PBS) is soaked 30 minutes, with the mesh for reaching sterilization and thoroughly removing the solvent composition of possible residual in PU/PVDF electrospinning films
's；
B, each wound dressing electrospinning film is implanted into Sprague-Dawley (SD) subcutaneous rat respectively, rat no abnormality seen
Behavior expression and obvious toxic reaction；Compared with before implantation, the PU/PVDF electrospinnings film of taking-up in appearance without significant change,
Change without damaged, flexible etc.；The cell on wound dressing electrospinning film surface is the fibroblast of normal growth.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
God any modification, equivalent substitution and improvements done etc., should be included within the scope of protection of the invention with principle.
Claims (10)
1. a kind of wound dressing, it is characterised in that the wound dressing includes medicine and pharmaceutical carrier, and the pharmaceutical carrier is to receive
Rice tunica fibrosa, the preparing raw material of the nano fibrous membrane include fibrinogen and polycaprolactone.
2. wound dressing according to claim 1, it is characterised in that the nano fibrous membrane prepare with the following method and
Into：
A) electrospinning liquid is prepared:Fibrinogen is dissolved in the mixed solvent, is prepared into 85-125mg/ml fibrin stoste；
Polycaprolactone is dissolved in the solution for being made that mass concentration is 12%-26% in organic solvent；Then by the fibrinogen of preparation
Liquid is well mixed with polycaprolactone solution, obtains fibrinogen/polycaprolactone mixing electrospinning liquid；
B) electrospinning:Step a fibrinogen/polycaprolactone is mixed into electrospinning liquid electrospinning device in applied voltage 15-
Electrospinning is carried out under conditions of it is 10-20cm that 25kv, electrospinning flow velocity, which be 3-7ml/h, receives distance, rotating speed is 400-600r/min,
Obtain nano fibrous membrane；
C) the nano fibrous membrane dry sterilization that step b) is prepared.
3. wound dressing according to claim 2, it is characterised in that mixed solvent is that volume ratio is 9 in the step a)：
1 organic solvent liquid mixing compatible with water；
The organic solvent of the in the mixed solvent is hexafluoroisopropanol；
The aqueous phase solution of the in the mixed solvent is in distilled water, MEM minimal mediums, physiological saline, phosphate buffer
It is at least one.
4. the preparation method of wound dressing according to claim 2, it is characterised in that the organic solvent of the step a) is
At least one of hexafluoroisopropanol, chloroform, dimethylformamide, tetrahydrofuran and trifluoroethanol.
5. according to the wound dressing described in any one of Claims 1 to 4, it is characterised in that the medicine be penicillins, fill in
At least one of meter Song, EGF, TGF, vascular endothelial growth factor, nerve growth factor.
6. the preparation method of a kind of wound dressing as described in any one of Claims 1 to 5, it is characterised in that including following step
Suddenly：
A) electrospinning liquid is prepared:Fibrinogen is dissolved in the mixed solvent, is prepared into the fiber that mass fraction is 85-125mg/ml
Albumen stoste；Polycaprolactone is dissolved in the solution for being made that mass fraction is 12%-26% in organic solvent；Then by the fibre of preparation
Fibrillarin stoste is well mixed with polycaprolactone solution, obtains fibrinogen/polycaprolactone mixing electrospinning liquid；And in the fibre
Medicine is added in fibrillarin original/polycaprolactone mixing electrospinning liquid, fibrinogen/polycaprolactone/medicine mixing electrospinning liquid is made；
B) electrospinning:Step a) fibrinogen/polycaprolactone/medicine is mixed into electrospinning liquid to be powered up outside with electrospinning device
Carried out under conditions of it is 10-20cm that pressure 15-25kv, electrospinning flow velocity, which be 3-7ml/h, receives distance, rotating speed is 400-600r/min
Electrospinning, obtain wound dressing；
C) the wound dressing dry sterilization for obtaining step b).
7. the preparation method of wound dressing according to claim 6, it is characterised in that mixed solvent is in the step a)
Volume ratio is 9：1 organic solvent liquid mixing compatible with water；
The organic solvent of the in the mixed solvent is hexafluoroisopropanol；
The aqueous phase solution of the in the mixed solvent is in distilled water, MEM minimal mediums, physiological saline, phosphate buffer
It is at least one.
8. the preparation method of wound dressing according to claim 6, it is characterised in that the organic solvent of the step a) is
At least one of hexafluoroisopropanol, chloroform, dimethylformamide, tetrahydrofuran and trifluoroethanol.
9. the preparation method of wound dressing according to claim 6, it is characterised in that：The medicine be penicillins,
At least one in Sai meter Song, EGF, TGF, vascular endothelial growth factor, nerve growth factor
Kind.
10. the preparation method of wound dressing according to claim 6, it is characterised in that the drying in the step c) is gone out
Wound dressing is is dried in vacuo by bacterium, then with Co irradiation or ultraviolet sterilization.
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| CN111962210A (en) * | 2020-06-22 | 2020-11-20 | 华南理工大学 | Polycaprolactone/methacryloylated elastin nanofiber composite membrane and preparation method and application thereof |
| CN113274538A (en) * | 2021-05-28 | 2021-08-20 | 中国科学院过程工程研究所 | bFGF slow-release nano dressing with wound surface active repair function and preparation method and application thereof |
| CN115467092A (en) * | 2022-09-15 | 2022-12-13 | 大连医科大学附属第二医院 | Preparation method of supported dimethyloxalglycine nanofiber |
| WO2023143335A1 (en) * | 2022-01-30 | 2023-08-03 | 上海松力生物技术有限公司 | Hydrophilic electrostatic spinning implant for inducing regeneration of skin tissues |
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