CN110433325A - A kind of protide high polymer nanometer fiber hemostatic material and its preparation method and application - Google Patents
A kind of protide high polymer nanometer fiber hemostatic material and its preparation method and application Download PDFInfo
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- CN110433325A CN110433325A CN201910680967.1A CN201910680967A CN110433325A CN 110433325 A CN110433325 A CN 110433325A CN 201910680967 A CN201910680967 A CN 201910680967A CN 110433325 A CN110433325 A CN 110433325A
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- Prior art keywords
- protide
- hemostatic material
- high polymer
- nanometer fiber
- spinning
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- 239000000463 material Substances 0.000 title claims abstract description 74
- 230000002439 hemostatic effect Effects 0.000 title claims abstract description 53
- 239000000835 fiber Substances 0.000 title claims abstract description 40
- 229920000642 polymer Polymers 0.000 title claims abstract description 17
- 238000002360 preparation method Methods 0.000 title claims abstract description 16
- 230000010287 polarization Effects 0.000 claims abstract description 43
- 238000009987 spinning Methods 0.000 claims abstract description 38
- 239000002121 nanofiber Substances 0.000 claims abstract description 35
- 238000000034 method Methods 0.000 claims abstract description 25
- 238000010041 electrostatic spinning Methods 0.000 claims abstract description 23
- 229920002521 macromolecule Polymers 0.000 claims abstract description 13
- 230000008569 process Effects 0.000 claims abstract description 13
- 230000005684 electric field Effects 0.000 claims abstract description 10
- 238000004090 dissolution Methods 0.000 claims abstract description 3
- 229920000159 gelatin Polymers 0.000 claims description 23
- 235000019322 gelatine Nutrition 0.000 claims description 23
- 230000001954 sterilising effect Effects 0.000 claims description 17
- 238000001035 drying Methods 0.000 claims description 13
- 108010010803 Gelatin Proteins 0.000 claims description 12
- 239000008273 gelatin Substances 0.000 claims description 12
- 235000011852 gelatine desserts Nutrition 0.000 claims description 12
- 102000008186 Collagen Human genes 0.000 claims description 9
- 108010035532 Collagen Proteins 0.000 claims description 9
- 108010022355 Fibroins Proteins 0.000 claims description 8
- 229920001436 collagen Polymers 0.000 claims description 8
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 claims description 8
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 claims description 7
- 239000002904 solvent Substances 0.000 claims description 7
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 6
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 5
- IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 claims description 5
- 238000001523 electrospinning Methods 0.000 claims description 5
- BYEAHWXPCBROCE-UHFFFAOYSA-N 1,1,1,3,3,3-hexafluoropropan-2-ol Chemical compound FC(F)(F)C(O)C(F)(F)F BYEAHWXPCBROCE-UHFFFAOYSA-N 0.000 claims description 4
- ZWEHNKRNPOVVGH-UHFFFAOYSA-N 2-Butanone Chemical compound CCC(C)=O ZWEHNKRNPOVVGH-UHFFFAOYSA-N 0.000 claims description 4
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 claims description 4
- JHIVVAPYMSGYDF-UHFFFAOYSA-N cyclohexanone Chemical compound O=C1CCCCC1 JHIVVAPYMSGYDF-UHFFFAOYSA-N 0.000 claims description 4
- XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 claims description 4
- 235000019253 formic acid Nutrition 0.000 claims description 4
- 235000019441 ethanol Nutrition 0.000 claims description 3
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims description 3
- 150000003254 radicals Chemical class 0.000 claims description 3
- 102000016942 Elastin Human genes 0.000 claims description 2
- 108010014258 Elastin Proteins 0.000 claims description 2
- 229920001872 Spider silk Polymers 0.000 claims description 2
- RHQDFWAXVIIEBN-UHFFFAOYSA-N Trifluoroethanol Chemical compound OCC(F)(F)F RHQDFWAXVIIEBN-UHFFFAOYSA-N 0.000 claims description 2
- 235000011054 acetic acid Nutrition 0.000 claims description 2
- 229920002549 elastin Polymers 0.000 claims description 2
- VBZWSGALLODQNC-UHFFFAOYSA-N hexafluoroacetone Chemical compound FC(F)(F)C(=O)C(F)(F)F VBZWSGALLODQNC-UHFFFAOYSA-N 0.000 claims description 2
- 150000002576 ketones Chemical class 0.000 claims description 2
- 229920001184 polypeptide Polymers 0.000 claims description 2
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 2
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 2
- 235000019260 propionic acid Nutrition 0.000 claims description 2
- IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 claims description 2
- 238000004659 sterilization and disinfection Methods 0.000 claims description 2
- 238000001291 vacuum drying Methods 0.000 claims description 2
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 claims 3
- 241000208340 Araliaceae Species 0.000 claims 1
- OHLUUHNLEMFGTQ-UHFFFAOYSA-N N-methylacetamide Chemical compound CNC(C)=O OHLUUHNLEMFGTQ-UHFFFAOYSA-N 0.000 claims 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 claims 1
- 235000003140 Panax quinquefolius Nutrition 0.000 claims 1
- 108010055615 Zein Proteins 0.000 claims 1
- 229920002494 Zein Polymers 0.000 claims 1
- 235000008434 ginseng Nutrition 0.000 claims 1
- 239000002070 nanowire Substances 0.000 claims 1
- 102000015081 Blood Coagulation Factors Human genes 0.000 abstract description 7
- 108010039209 Blood Coagulation Factors Proteins 0.000 abstract description 7
- 239000003114 blood coagulation factor Substances 0.000 abstract description 7
- 230000000025 haemostatic effect Effects 0.000 abstract description 6
- 239000002657 fibrous material Substances 0.000 abstract description 3
- 208000015181 infectious disease Diseases 0.000 abstract description 3
- 238000009826 distribution Methods 0.000 description 16
- 239000001828 Gelatine Substances 0.000 description 11
- 230000023555 blood coagulation Effects 0.000 description 11
- 206010052428 Wound Diseases 0.000 description 10
- 208000027418 Wounds and injury Diseases 0.000 description 10
- 239000008280 blood Substances 0.000 description 10
- 210000004369 blood Anatomy 0.000 description 10
- 230000023597 hemostasis Effects 0.000 description 9
- 102000009123 Fibrin Human genes 0.000 description 7
- 108010073385 Fibrin Proteins 0.000 description 7
- BWGVNKXGVNDBDI-UHFFFAOYSA-N Fibrin monomer Chemical compound CNC(=O)CNC(=O)CN BWGVNKXGVNDBDI-UHFFFAOYSA-N 0.000 description 7
- 238000003149 assay kit Methods 0.000 description 7
- 239000012496 blank sample Substances 0.000 description 7
- 238000005422 blasting Methods 0.000 description 7
- 239000013068 control sample Substances 0.000 description 7
- 238000001514 detection method Methods 0.000 description 7
- 229950003499 fibrin Drugs 0.000 description 7
- 238000005259 measurement Methods 0.000 description 7
- 238000004321 preservation Methods 0.000 description 7
- 238000013019 agitation Methods 0.000 description 5
- 108010062466 Enzyme Precursors Proteins 0.000 description 4
- 102000010911 Enzyme Precursors Human genes 0.000 description 4
- 239000004593 Epoxy Substances 0.000 description 4
- 150000001335 aliphatic alkanes Chemical class 0.000 description 4
- 230000015271 coagulation Effects 0.000 description 4
- 238000005345 coagulation Methods 0.000 description 4
- SYUXAJSOZXEFPP-UHFFFAOYSA-N glutin Natural products COc1c(O)cc2OC(=CC(=O)c2c1O)c3ccccc3OC4OC(CO)C(O)C(O)C4O SYUXAJSOZXEFPP-UHFFFAOYSA-N 0.000 description 4
- 229910052751 metal Inorganic materials 0.000 description 4
- 239000002184 metal Substances 0.000 description 4
- 239000002059 nanofabric Substances 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 230000004913 activation Effects 0.000 description 3
- 239000008367 deionised water Substances 0.000 description 3
- 229910021641 deionized water Inorganic materials 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 230000008961 swelling Effects 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- FKNQFGJONOIPTF-UHFFFAOYSA-N Sodium cation Chemical compound [Na+] FKNQFGJONOIPTF-UHFFFAOYSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 229940030225 antihemorrhagics Drugs 0.000 description 2
- 210000001367 artery Anatomy 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 239000000470 constituent Substances 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- JMANVNJQNLATNU-UHFFFAOYSA-N oxalonitrile Chemical compound N#CC#N JMANVNJQNLATNU-UHFFFAOYSA-N 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 229910001415 sodium ion Inorganic materials 0.000 description 2
- 230000029663 wound healing Effects 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 1
- 229920001651 Cyanoacrylate Polymers 0.000 description 1
- 108010080865 Factor XII Proteins 0.000 description 1
- 102000000429 Factor XII Human genes 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- FXHOOIRPVKKKFG-UHFFFAOYSA-N N,N-Dimethylacetamide Chemical compound CN(C)C(C)=O FXHOOIRPVKKKFG-UHFFFAOYSA-N 0.000 description 1
- 108090000113 Plasma Kallikrein Proteins 0.000 description 1
- 102000003827 Plasma Kallikrein Human genes 0.000 description 1
- 208000007536 Thrombosis Diseases 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 229910021536 Zeolite Inorganic materials 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
- 230000002745 absorbent Effects 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 229960000182 blood factors Drugs 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 229940113088 dimethylacetamide Drugs 0.000 description 1
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 description 1
- 230000005686 electrostatic field Effects 0.000 description 1
- 238000001125 extrusion Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005297 material degradation process Methods 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 229910044991 metal oxide Inorganic materials 0.000 description 1
- 150000004706 metal oxides Chemical class 0.000 description 1
- WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical compound O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 description 1
- 239000012046 mixed solvent Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000001473 noxious effect Effects 0.000 description 1
- 239000002861 polymer material Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 239000004753 textile Substances 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 230000002792 vascular Effects 0.000 description 1
- 239000010457 zeolite Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/001—Use of materials characterised by their function or physical properties
- A61L24/0042—Materials resorbable by the body
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/04—Surgical adhesives or cements; Adhesives for colostomy devices containing macromolecular materials
- A61L24/10—Polypeptides; Proteins
- A61L24/102—Collagen
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/04—Surgical adhesives or cements; Adhesives for colostomy devices containing macromolecular materials
- A61L24/10—Polypeptides; Proteins
- A61L24/104—Gelatin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/04—Surgical adhesives or cements; Adhesives for colostomy devices containing macromolecular materials
- A61L24/10—Polypeptides; Proteins
- A61L24/106—Fibrin; Fibrinogen
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F4/00—Monocomponent artificial filaments or the like of proteins; Manufacture thereof
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F4/00—Monocomponent artificial filaments or the like of proteins; Manufacture thereof
- D01F4/02—Monocomponent artificial filaments or the like of proteins; Manufacture thereof from fibroin
-
- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M10/00—Physical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. ultrasonic, corona discharge, irradiation, electric currents, or magnetic fields; Physical treatment combined with treatment with chemical compounds or elements
-
- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M10/00—Physical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. ultrasonic, corona discharge, irradiation, electric currents, or magnetic fields; Physical treatment combined with treatment with chemical compounds or elements
- D06M10/001—Treatment with visible light, infrared or ultraviolet, X-rays
-
- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M10/00—Physical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. ultrasonic, corona discharge, irradiation, electric currents, or magnetic fields; Physical treatment combined with treatment with chemical compounds or elements
- D06M10/02—Physical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. ultrasonic, corona discharge, irradiation, electric currents, or magnetic fields; Physical treatment combined with treatment with chemical compounds or elements ultrasonic or sonic; Corona discharge
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/04—Materials for stopping bleeding
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/12—Nanosized materials, e.g. nanofibres, nanoparticles, nanowires, nanotubes; Nanostructured surfaces
-
- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M2101/00—Chemical constitution of the fibres, threads, yarns, fabrics or fibrous goods made from such materials, to be treated
- D06M2101/02—Natural fibres, other than mineral fibres
- D06M2101/10—Animal fibres
-
- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M2101/00—Chemical constitution of the fibres, threads, yarns, fabrics or fibrous goods made from such materials, to be treated
- D06M2101/02—Natural fibres, other than mineral fibres
- D06M2101/10—Animal fibres
- D06M2101/14—Collagen fibres
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Textile Engineering (AREA)
- Surgery (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Epidemiology (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Plasma & Fusion (AREA)
- Physics & Mathematics (AREA)
- Materials Engineering (AREA)
- Materials For Medical Uses (AREA)
Abstract
The invention discloses a kind of protide high polymer nanometer fiber hemostatic materials and its preparation method and application, and protide macromolecule dissolution is configured to spinning solution；Protide macromolecule is prepared by electrostatic spinning the nanofiber of tridimensional network；The nanofiber of tridimensional network is polarised in polarized electric field；The nanofiber of tridimensional network after polarization process is dried and sterilizes, and obtains protide high polymer nanometer fiber hemostatic material.The present invention obtains material, and there is special structure can effectively activate coagulation factor, improves the haemostatic effect of unit volume fibrous material, reduces the infection probability of wound location.This material has good biocompatibility and biodegradability, can be used as a kind of hemostatic material.
Description
Technical field
The present invention relates to nanofiber manufacturing field, specially a kind of protide high polymer nanometer fiber hemostatic material and its
Preparation method and application.
Background technique
Traditional hemostatic material (such as cotton yarn, bandage, tourniquet) is existing for irregular shape, deep, narrow, arteriorrhexis etc.
The haemostatic effect of the common wound in field is very unsatisfactory.Inorganic mineral such as zeolite hemostatic material is difficult to use under windy environment, and
It may cause third-degree burn, or even residual vascular and lung lead to thrombus.ɑ-cyanoacrylate hemostatic material degradation process
In then inevitably release the noxious materials such as a small amount of cyanogen and formaldehyde, cause tissue inflammation.With the development of science and technology, people by
Gradually center of gravity is transferred in novel bioabsorbable polymer material exploitation.Natural protide high molecular material (such as collagen, gelatin,
Fibroin etc.) there is good biocompatibility and degradability, it is the emphasis of researchers' concern.Collagen electrospinning film has
Porosity is high, and the good advantage of water absorbing properties, can be used for clinical hemostasis material, (such as Cheng Weilu, Li Hui, He Jinmei are degradable compound
The preparation of hemostasis collagen protein sponge and biologic applications performance study [J] chemistry and bonding, 2016,38 (4): 231-234).In
(He Meizhen, Chen little Yan, Zheng Jingjing gelfoam are surveyed in the invasive artery of ICU for point of puncture application after the invasive artery of ICU surveys pressed pipe
Application [J] after pressed pipe in point of puncture hemostasis nurses journal .2015 (22): embodiment of 40-41.) and in tooth extraction wound stopping blooding
(Zhu Shaoping, Zhang Aiping sponeostan tooth extraction wound 290 effect observation [J] Shandong medicine .2003 (35) of hemostasis: 69-
69.) in, the simple of gelfoam hemostasis, economy and validity have obtained the approval of patient and medical staff.Sorada etc.
(Sorada K,Siriporn D,Juthamas R.Aninnovative bi-layered wound dressing made
of silk and gelatin for accelerated woundhealing.International Journal of
Pharmaceutics, 2012,436:141-153.) research fibroin albumen and gelatin bilayer hemostatic material and it is commercial only
Blood material promotes wound healing compared to that can significantly reduce wound area.However it is higher and higher to hemostatic material in medical field
Under performance requirement, the hemostatic material of protide still has that anthemorrhagic speed is slow, to the drawing ability and surface of a wound group of blood constituent
The shortcoming of poor adhesive force is knitted, therefore how to improve the anthemorrhagic speed of protide macromolecule hemostatic material, is allowed to when contacting wound
Coagulation factor can be quickly activated, the generation of fibrin ferment is accelerated, promoting the formation of sludged blood is the pass for opening up such material application
Key.
Some researches show that the surface charge of material can influence the selective absorption and sedimentation of albumen, promote blood coagulation system
Activation, the especially material with negative charged surface can be activated solidifying in blood coagulation system by its surface charge abundant
Blood factor Ⅻ, and the factor be cause one of key factor of intrinsic coagulation system starting (OstomelTA, ShiQ,
Stoimenov PK,et al.Metal oxide surface charge mediated
hemostasis.Langmuir.2007,23:11233-11238；Shariat-MadarZ,Mahdi F,Schmaier
AH.Assembly and activation of the Plasmakallikrein/kininsystem:a new
interpretation.International Immunopharmaeol.2002,2(13-14):1841-1849).Chen Lu etc.
Studies have shown that its anthemorrhagic performance of methylated collagen positively charged by surface modification be greatly improved (Chen Lu,
Li Xia, Jiang Bo modify styptic activity research [J] China and foreign countries medical treatment .2013,32 (23): 130-131 of collagen).Protein is most
Important biology polarization high molecular material, has the ability (summer that can be stored real charge for a long time and (or) keep polarized state
Zhong Fu Biomaterials of Electret [J] material Leader .1994 (1): 48-52.).Under the action of high voltage electric field, carboxyl, amino
Equal charged groups tend to orderly polarization offset, and fetter the charge intensified in a large amount of electric fields, and fiber surface is made to form difference
Potential areas.Electrostatic spinning is a kind of nanofiber technology of preparing of simple process, relative to sponge hemostatic material, nanofiber
The material specific surface area of structure is bigger, more with the contact area of blood, and hemostatic composition is easier to adhesion and aggregation in fiber surface, from
And shorten bleeding stopping period, raising haemostatic effect (preparation of Liu Xuefeng wound hemostasis nano-fiber material and the Nanjing performance [D]:
Southeast China University, 2011:1-51).The polarization process of the presence of high-voltage electrostatic field and tunica fibrosa later what is more is also polarity material
Material provides polarization and captures charge offer may.
Protide high molecular material constructs a kind of molecular chain orientation arrangement by electrostatic spinning technique, and fiber surface distribution is more
The nano-fiber material of the three-dimensional microcosmic network structure of kind hydrophilic radical, then by polarization process, make material internal polarization orientation,
Surface is distributed polarization charge, so that absorption moisture that can be instantaneous in wound hemostasis, adsorbs blood constituent, increase in subrange
Fluid viscosity is healed, and quickly activates coagulation factor, accelerates the generation of fibrin ferment, promotes coagulation process, to improve material
Anthemorrhagic speed.Therefore the protide biological absorbable hemostatic material of this kind of energy quick-acting haemostatic powder is in terms of clinical medicine and hemostasis
There to be more significant advantage.
Summary of the invention
The infection risk that the purpose of the present invention is may cause for protide macromolecule hemostatic material, provides a kind of raising
The preparation method of the nanofiber hemostatic material of unit volume haemostatic effect.
Another object of the present invention is to provide a kind of protide high polymer nanometer fiber hemostatic materials.
In order to solve the above-mentioned technical problem, the technical scheme adopted by the invention is that:
A kind of preparation method of protide high polymer nanometer fiber hemostatic material, includes the following steps:
(1) protide macromolecule dissolution is configured to spinning solution；
(2) protide macromolecule is prepared by electrostatic spinning the nanofiber of tridimensional network；
(3) nanofiber of tridimensional network is polarised in polarized electric field；
(4) nanofiber of the tridimensional network after polarization process is dried and sterilizes, and obtains protide macromolecule
Nanofiber hemostatic material.
The method of the electrostatic spinning is, using single or mixed liquor as solvent, using protide macromolecule as solute, adjusts
Spinning parameter, be prepared molecular chain orientation arrangement, fiber surface be distributed a variety of hydrophilic radicals with three-dimensional net structure
Nano-fiber material.
The solvent is water, formic acid, acetic acid, propionic acid, trifluoroethanol, hexafluoroisopropanol, Hexafluoro acetone, dimethyl formyl
What one of amine, dimethyl acetamide, acetone, ketone, butanone, cyclohexanone, ethyl alcohol, methanol or more than one solvents formed
Mixed solvent；
The protide macromolecule is collagen, gelatin, fibroin albumen, spider silk fibroin, elastin laminin, the molten egg of corn alcohol
One or more than one kinds of compositions in white, polypeptide；Concentration of dope is between 2%~40%.
The spinning parameter, including concentration of dope is between 2%~40%, spinning voltage is between 5~90kV, spinning
Flight lead between 5~30cm, spinning solution temperature is at 0~70 DEG C, 0~65 DEG C of environment temperature.
Electrostatic spinning mode includes spininess spinning, spiral textile, metal silk spinning, sheet metal spinning and metal salient point spinning etc..
Polarization process described in step (3), refers in polarized electric field, and electrospinning protide fibrous inside forms polarization charge
Or dipole, carboxyl and the orderly polarization offset of amino charged group, and the charge intensified in a large amount of electric fields is adsorbed, it is formed in fiber
Space bound charge.
The polarization mode includes corona polarizing, thermal poling, the polarization of soft X-ray, radiation-polarizing and magnetic polarization.
Step (4) described drying, is dried in a vacuum drying oven, and at 35-80 DEG C, drying time is no more than drying temperature
48h。
The sterilizing, including ethylene oxide sterilizing, ultraviolet sterilization or radiosterilization.
Present invention protide high polymer nanometer fiber hemostatic material obtained be by fibre diameter between 0.05~5 μm
Micro nanometer fiber composition, with high voidage and specific surface area three-dimensional network laminated structure.As hemostatic material
Heterogeneous (coarse) nanofiber three-dimensional structure material, can effectively improve hydrophilic absorbent；Band heterogeneous
The carboxyl of ammeter face and fiber surface, which reacts bring with sodium ion, stimulates the coagulation factor that can effectively activate in blood coagulation system,
The generation for promoting fibrin ferment, to accelerate coagulation process.The present invention is constructed a kind of with special construction by electrostatic spinning process
With the nanofiber hemostatic material of the three-dimensional net structure of function.This fibrous material, since the strength of electrostatic force is drawn when electrospinning
It stretches, molecular chain orientation arrangement, pulp freeness is big, the group of surface integrated distribution multiple functions；Due to orientation when polarization
And compact arranged molecular structure, anisotropy is big, is easier to that gelatin chains is made to form polarization charge or dipole, carboxyl and ammonia
The charged groups such as base can also tend to orderly polarization offset, and adsorb the charge intensified in a large amount of electric fields, become intrastitial sky
Between bound charge, thus formed nanofiber composition non-homogeneous charged surface, to a certain extent activate blood coagulation system in
Coagulation factor, promote the generation of fibrin ferment, to accelerate coagulation process, achieve the purpose that quick-acting haemostatic powder.
Compared with prior art, the beneficial effects of the present invention are:
The present invention controls the microstructure of fiber by regulation electrospinning parameters, while arranging molecular chain orientation more
More polar groups (such as carboxyl) is exposed in fiber surface, and carboxyl is reacted with sodium ion can stimulate activation coagulation factor.Later
Polarization process, so that fibrous inside and surface is obtained polarization orientation and capture space charge, be made of to obtain nanofiber
Non-homogeneous charged surface.This special structure can effectively activate coagulation factor, improve stopping for unit volume fibrous material
Blood effect reduces the infection probability of wound location.This material has good biocompatibility and biodegradability, can be with
As a kind of Absorbable hemostatic material.
Detailed description of the invention
The following drawings is a part of the application, is to further elucidate to of the invention, but do not constitute to limit of the invention
It is fixed, in which:
Fig. 1 is the preparation facilities schematic diagram in embodiment 2；
Fig. 2 is that the hemostatic material fibre structure SEM in embodiment 2 is intended to；
Fig. 3 is hemostatic material pictorial diagram in embodiment 2.
Specific embodiment
Below with reference to embodiment, the present invention will be described in further detail.It should be appreciated that specific reality described herein
It applies example to be only used to explain the present invention, be not intended to limit the present invention.
Embodiment 1
(1) it weighs 25g pigskin gelatin to be added in 183g deionized water, is swollen 25min at normal temperature；
(2) gelatin solution of swelling is put into magnetic agitation 30min in 55 DEG C of water-bath, made it completely dissolved；
It (3) is 40-45 DEG C in spinning solution temperature, environment temperature is 30-35 DEG C, in the case that hot-air is annular cross air blasting
Electrostatic spinning, lace curtaining revolving speed are 8r/min；
(4) spiral electrostatic spinning mode, voltage 60kv, electric current 0.12mA are used, spinning distance is 15cm；
(5) polarization method used is Filamentous corona polarizing, and voltage 60kv, electric current 3mA, polarization distance is 10cm；
(6) glutin nano fabric film is obtained after drying for 24 hours, to be sealed with sterilizing bag, epoxy second in 40 DEG C of vacuum ovens
Alkane sterilizing is placed on 4 DEG C of preservations；
(7) detection spinning fibre diameter is in normal distribution, and main distribution is 320-1100nm；
(8) gelatine nano fiber hemostatic material 0.03g, existing market control sample 0.03g and blank sample are taken, according to 0.01g/
Pooled plasma 3.0mL is added in the ratio of mL, and 3 Duplicate Samples are arranged, is placed under (37 ± 1) DEG C biochemical cultivation case and incubates 1h, with solidifying
Blood zymogen time assay kit and Standard for semi-Automated Blood Coagulation Analyzer measure its setting time.Measurement result are as follows: blank group 9.7 ±
0.1s, market samples control group are 20.2 ± 3.3s, and gelatine nano fiber hemostatic material is 8.4 ± 0.2s.Have between three aobvious
Write difference.
Embodiment 2
Using device shown in FIG. 1.
(1) it weighs 18g ox bone gelatin to be added in 207g deionized water, is swollen 20min at normal temperature；
(2) gelatin solution of swelling is put into magnetic agitation 30min in 60 DEG C of water-bath, made it completely dissolved；
It (3) is 40-45 DEG C in spinning solution temperature, environment temperature is 30-35 DEG C, in the case that hot-air is annular cross air blasting
Electrostatic spinning, lace curtaining revolving speed are 10r/min；
(4) spiral electrostatic spinning mode, voltage 60kv, electric current 0.12mA are used, spinning distance is 17cm；
(5) polarization method used is Filamentous corona polarizing, and voltage 60kv, electric current 3mA, polarization distance is 10cm；
(6) glutin nano fabric film is obtained after drying for 24 hours, to be sealed with sterilizing bag, epoxy second in 40 DEG C of vacuum ovens
Alkane sterilizing is placed on 4 DEG C of preservations；
(7) detection spinning fibre diameter is in normal distribution, and main distribution is 260-1300nm；
The hemostatic material fibre structure SEM that embodiment 2 obtains is intended to as shown in Fig. 2, pictorial diagram is as shown in Figure 3.
(8) gelatine nano fiber hemostatic material 0.06g, existing market control sample 0.06g and blank sample are taken, according to 0.02g/
Pooled plasma 3.0mL is added in the ratio of mL, and 3 Duplicate Samples are arranged, is placed under (37 ± 1) DEG C biochemical cultivation case and incubates 1h, with solidifying
Blood zymogen time assay kit and Standard for semi-Automated Blood Coagulation Analyzer measure its setting time.Measurement result are as follows: blank group 9.7 ±
0.1s, market samples control group are 28.3 ± 5.8s, and gelatine nano fiber hemostatic material is 8.1 ± 0.1s.Have between three aobvious
Write difference.
Embodiment 3
(1) it weighs 18g collagen to be added in 282g hexafluoroisopropanol, magnetic agitation dissolves 60min at normal temperature；
(2) solution stand bubble removing 30min, clarify it completely；
It (3) is 20-25 DEG C in spinning solution temperature, environment temperature is 25-30 DEG C, in the case that hot-air is annular cross air blasting
Electrostatic spinning, lace curtaining revolving speed are 10r/min；
(4) Multi needle electrostatic spinning mode, voltage 30kv, electric current 0.12mA are used, extrusion output is the hole 0.5ml/h/,
Spinning distance is 15cm；
(5) polarization method used is needle-shaped corona polarizing, and voltage 60kv, electric current 3mA, polarization distance is 10cm；
(6) nano fibrous membrane is obtained after drying for 24 hours, to be sealed with sterilizing bag, ethylene oxide goes out in 40 DEG C of vacuum ovens
Bacterium is placed on 4 DEG C of preservations；
(7) detection spinning fibre diameter is in normal distribution, and main distribution is 350-1200nm；
(8) nanofiber hemostatic material 0.06g, existing market control sample 0.06g and blank sample are taken, according to 0.02g/mL's
Pooled plasma 3.0mL is added in ratio, and 3 Duplicate Samples are arranged, is placed under (37 ± 1) DEG C biochemical cultivation case and incubates 1h, use fibrin ferment
Former time assay kit and Standard for semi-Automated Blood Coagulation Analyzer measure its setting time.Measurement result are as follows: 9.7 ± 0.1s of blank group,
Market samples control group is 28.3 ± 5.8s, and gelatine nano fiber hemostatic material is 8.6 ± 0.2s.There is significance difference between three
It is different.
Embodiment 4
(1) it weighs 18g collagen to be added in 282g hexafluoroisopropanol, magnetic agitation dissolves 60min at normal temperature；
(2) solution stand bubble removing 30min, clarify it completely；
It (3) is 20-25 DEG C in spinning solution temperature, environment temperature is 25-30 DEG C, in the case that hot-air is annular cross air blasting
Electrostatic spinning, lace curtaining revolving speed are 8r/min；
(4) spiral electrostatic spinning mode, voltage 80kv, electric current 0.25mA are used, spinning distance is 20cm；
(5) polarization method used is Filamentous corona polarizing, and voltage 60kv, electric current 3mA, polarization distance is 10cm；
(6) glutin nano fabric film is obtained after drying for 24 hours, to be sealed with sterilizing bag, epoxy second in 40 DEG C of vacuum ovens
Alkane sterilizing is placed on 4 DEG C of preservations；
(7) detection spinning fibre diameter is in normal distribution, and main distribution is 300-1100nm；
(8) gelatine nano fiber hemostatic material 0.03g, existing market control sample 0.03g and blank sample are taken, according to 0.01g/
Pooled plasma 3.0mL is added in the ratio of mL, and 3 Duplicate Samples are arranged, is placed under (37 ± 1) DEG C biochemical cultivation case and incubates 1h, with solidifying
Blood zymogen time assay kit and Standard for semi-Automated Blood Coagulation Analyzer measure its setting time.Measurement result are as follows: blank group 9.7 ±
0.1s, market samples control group are 20.2 ± 3.3s, and gelatine nano fiber hemostatic material is 7.8 ± 0.1s.Have between three aobvious
Write difference.
Embodiment 5
(1) it weighs the spongy fibroin fiber albumen of 90g to be added in 180g anhydrous formic acid, dissolves 180min at normal temperature；
(2) solution stand bubble removing 30min, clarify it completely；
It (3) is 20-25 DEG C in spinning solution temperature, environment temperature is 25-30 DEG C, in the case that hot-air is annular cross air blasting
Electrostatic spinning, lace curtaining revolving speed are 12r/min；
(4) wire electrostatic spinning mode, voltage 45kv, electric current 0.10mA are used, spinning distance is 12cm；
(5) polarization method used is Filamentous corona polarizing, and voltage 60kv, electric current 3mA, polarization distance is 10cm；
(6) nano fibrous membrane is obtained after drying for 24 hours, to be sealed with sterilizing bag, ethylene oxide goes out in 40 DEG C of vacuum ovens
Bacterium is placed on 4 DEG C of preservations；
(7) detection spinning fibre diameter is in normal distribution, and main distribution is 300-1100nm；
(8) nanofiber hemostatic material 0.03g, existing market control sample 0.03g and blank sample are taken, according to 0.01g/mL's
Pooled plasma 3.0mL is added in ratio, and 3 Duplicate Samples are arranged, is placed under (37 ± 1) DEG C biochemical cultivation case and incubates 1h, use fibrin ferment
Former time assay kit and Standard for semi-Automated Blood Coagulation Analyzer measure its setting time.Measurement result are as follows: 9.7 ± 0.1s of blank group,
Market samples control group is 20.2 ± 3.3s, and gelatine nano fiber hemostatic material is 8.6 ± 0.4s.There is significance difference between three
It is different.
Embodiment 6
(1) it weighs the spongy fibroin fiber albumen of 90g to be added in 180g anhydrous formic acid, dissolves 180min at normal temperature；
(2) solution stand bubble removing 30min, clarify it completely；
It (3) is 20-25 DEG C in spinning solution temperature, environment temperature is 25-30 DEG C, in the case that hot-air is annular cross air blasting
Electrostatic spinning, lace curtaining revolving speed are 12r/min；
(4) sheet metal electrostatic spinning mode, voltage 60kv, electric current 0.12mA are used, spinning distance is 15cm；
(5) polarization method used is soft X-ray polarization, and voltage 40kv, electric current 6mA, polarization distance is 10cm, vertically
Irradiation intensity 10keV；
(6) nano fibrous membrane is obtained after drying for 24 hours, to be sealed with sterilizing bag, ethylene oxide goes out in 40 DEG C of vacuum ovens
Bacterium is placed on 4 DEG C of preservations；
(7) detection spinning fibre diameter is in normal distribution, and main distribution is 320-1100nm；
(8) nanofiber hemostatic material 0.03g, existing market control sample 0.03g and blank sample are taken, according to 0.01g/mL's
Pooled plasma 3.0mL is added in ratio, and 3 Duplicate Samples are arranged, is placed under (37 ± 1) DEG C biochemical cultivation case and incubates 1h, use fibrin ferment
Former time assay kit and Standard for semi-Automated Blood Coagulation Analyzer measure its setting time.Measurement result are as follows: 9.7 ± 0.1s of blank group,
Market samples control group is 20.2 ± 3.3s, and gelatine nano fiber hemostatic material is 7.6 ± 0.1s.There is significance difference between three
It is different.
Embodiment 7
(1) it weighs 25g pigskin gelatin to be added in 200g deionized water, is swollen 25min at normal temperature；
(2) gelatin solution of swelling is put into magnetic agitation 30min in 55 DEG C of water-bath, made it completely dissolved；
It (3) is 40-45 DEG C in spinning solution temperature, environment temperature is 30-35 DEG C, in the case that hot-air is annular cross air blasting
Electrostatic spinning, lace curtaining revolving speed are 8r/min；
(4) spiral electrostatic spinning mode, voltage 60kv, electric current 0.12mA are used, spinning distance is 15cm；
(5) polarization method used is thermal poling, and voltage 40kv, electric current 2mA, polarization distance is 10cm, polarization temperature
Degree is 70 DEG C；
(6) glutin nano fabric film is obtained after drying for 24 hours, to be sealed with sterilizing bag, epoxy second in 40 DEG C of vacuum ovens
Alkane sterilizing is placed on 4 DEG C of preservations；
(7) detection spinning fibre diameter is in normal distribution, and main distribution is 320-1100nm；
(8) gelatine nano fiber hemostatic material 0.03g, existing market control sample 0.03g and blank sample are taken, according to 0.01g/
Pooled plasma 3.0mL is added in the ratio of mL, and 3 Duplicate Samples are arranged, is placed under (37 ± 1) DEG C biochemical cultivation case and incubates 1h, with solidifying
Blood zymogen time assay kit and Standard for semi-Automated Blood Coagulation Analyzer measure its setting time.Measurement result are as follows: blank group 9.7 ±
0.1s, market samples control group are 20.2 ± 3.3s, and gelatine nano fiber hemostatic material is 8.3 ± 0.2s.Have between three aobvious
Write difference.
Claims (9)
1. a kind of preparation method of protide high polymer nanometer fiber hemostatic material, which is characterized in that included following steps:
(1) protide macromolecule dissolution is configured to spinning solution；
(2) protide macromolecule is prepared by electrostatic spinning the nanofiber of tridimensional network；
(3) nanofiber of tridimensional network is polarised in polarized electric field；
(4) nanofiber of the tridimensional network after polarization process is dried and sterilizes, and obtains protide high molecular nanometer
Fiber hemostatic material.
2. a kind of preparation method of protide high polymer nanometer fiber hemostatic material according to claim 1, which is characterized in that
The method of the electrostatic spinning is, using single or mixed liquor as solvent, using protide macromolecule as solute, adjusts spinning ginseng
Molecular chain orientation arrangement is prepared in number, fiber surface is distributed the Nanowires with three-dimensional net structure of a variety of hydrophilic radicals
Tie up material.
3. a kind of preparation method of protide high polymer nanometer fiber hemostatic material according to claim 2, feature exist
In the solvent is water, formic acid, acetic acid, propionic acid, trifluoroethanol, hexafluoroisopropanol, Hexafluoro acetone, dimethylformamide, two
The mixing of one of methylacetamide, acetone, ketone, butanone, cyclohexanone, ethyl alcohol, methanol or more than one solvents composition
Solvent；
The protide macromolecule be collagen, gelatin, fibroin albumen, spider silk fibroin, elastin laminin, zeins,
One or more than one kinds of compositions in polypeptide；
The spinning parameter, including concentration of dope is between 2%~40%, spinning voltage between 5~90kV, spinning away from
From between 5~30cm, spinning solution temperature is at 0~70 DEG C, 0~65 DEG C of environment temperature.
4. a kind of preparation method of protide high polymer nanometer fiber hemostatic material according to claim 1, which is characterized in that
The polarization process, refers in polarized electric field, and electrospinning protide fibrous inside forms polarization charge or dipole, carboxyl and
The orderly polarization offset of amino charged group, and the charge intensified in a large amount of electric fields is adsorbed, form intrastitial space bound charge.
5. a kind of preparation method of protide high polymer nanometer fiber hemostatic material according to claim 4, which is characterized in that
The polarization mode includes corona polarizing, thermal poling, the polarization of soft X-ray, radiation-polarizing and magnetic polarization.
6. a kind of preparation method of protide high polymer nanometer fiber hemostatic material according to claim 1, which is characterized in that
The drying, is dried in a vacuum drying oven, and for drying temperature at 35-80 DEG C, drying time is no more than 48h.
7. a kind of preparation method of protide high polymer nanometer fiber hemostatic material according to claim 1, which is characterized in that
The sterilizing, including ethylene oxide sterilizing, ultraviolet sterilization or radiosterilization.
8. a kind of protide high polymer nanometer fiber hemostatic material, which is characterized in that by any method system of claims 1 to 7
Standby gained.
9. a kind of application of protide high polymer nanometer fiber hemostatic material according to claim 8, which is characterized in that make
For hemostatic material.
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