WO2006071895A3 - Nanoelectronic devices for dna detection, and recognition of polynucleotide sequences - Google Patents
Nanoelectronic devices for dna detection, and recognition of polynucleotide sequences Download PDFInfo
- Publication number
- WO2006071895A3 WO2006071895A3 PCT/US2005/047143 US2005047143W WO2006071895A3 WO 2006071895 A3 WO2006071895 A3 WO 2006071895A3 US 2005047143 W US2005047143 W US 2005047143W WO 2006071895 A3 WO2006071895 A3 WO 2006071895A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- electrodes
- dna sequence
- recognition
- target dna
- polynucleotide sequences
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
- G01N33/5438—Electrodes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y10/00—Nanotechnology for information processing, storage or transmission, e.g. quantum computing or single electron logic
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6825—Nucleic acid detection involving sensors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/403—Cells and electrode assemblies
- G01N27/414—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS
- G01N27/4145—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS specially adapted for biomolecules, e.g. gate electrode with immobilised receptors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/403—Cells and electrode assemblies
- G01N27/414—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS
- G01N27/4146—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS involving nanosized elements, e.g. nanotubes, nanowires
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01L—SEMICONDUCTOR DEVICES NOT COVERED BY CLASS H10
- H01L2924/00—Indexing scheme for arrangements or methods for connecting or disconnecting semiconductor or solid-state bodies as covered by H01L24/00
- H01L2924/0001—Technical content checked by a classifier
- H01L2924/0002—Not covered by any one of groups H01L24/00, H01L24/00 and H01L2224/00
Abstract
A nanotube device is configured as an electronic sensor for a target DNA sequence. A film of nanotubes is deposited over electrodes on a substrate. A solution of single-strand DNA is prepared so as to be complementary to a target DNA sequence. The DNA solution is deposited over the electrodes, dried, and removed from the substrate except in a region between the electrodes. The resulting structure includes strands of the desired DNA sequence in direct contact with nanotubes between opposing electrodes, to form a sensor that is electrically responsive to the presence of target DNA strands. Alternative assay embodiments are described which employ linker groups to attach ssDNA probes to the nanotube sensor device.
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2007549560A JP2008525822A (en) | 2004-12-28 | 2005-12-23 | Nanoelectronic device for DNA detection / recognition of polynucleotide sequences |
EP05855662A EP1831670A4 (en) | 2004-12-28 | 2005-12-23 | Nanoelectronic devices for dna detection, and recognition of polynucleotide sequences |
US14/961,572 US20160187276A1 (en) | 2004-05-14 | 2015-12-07 | Electrochemical nanosensors for biomolecule detection |
Applications Claiming Priority (16)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US63995404P | 2004-12-28 | 2004-12-28 | |
US60/639,954 | 2004-12-28 | ||
US65727505P | 2005-02-28 | 2005-02-28 | |
US60/657,275 | 2005-02-28 | ||
US66887905P | 2005-04-05 | 2005-04-05 | |
US60/668,879 | 2005-04-05 | ||
US11/212,026 US20070178477A1 (en) | 2002-01-16 | 2005-08-24 | Nanotube sensor devices for DNA detection |
US11/212,026 | 2005-08-24 | ||
US73090505P | 2005-10-27 | 2005-10-27 | |
US60/730,905 | 2005-10-27 | ||
US11/274,747 | 2005-11-15 | ||
US11/274,747 US20070208243A1 (en) | 2002-01-16 | 2005-11-15 | Nanoelectronic glucose sensors |
US73869405P | 2005-11-21 | 2005-11-21 | |
US60/738,694 | 2005-11-21 | ||
US74883405P | 2005-12-09 | 2005-12-09 | |
US60/748,834 | 2005-12-09 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2006071895A2 WO2006071895A2 (en) | 2006-07-06 |
WO2006071895A3 true WO2006071895A3 (en) | 2007-02-08 |
Family
ID=36615493
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2005/047143 WO2006071895A2 (en) | 2004-05-14 | 2005-12-23 | Nanoelectronic devices for dna detection, and recognition of polynucleotide sequences |
Country Status (3)
Country | Link |
---|---|
EP (1) | EP1831670A4 (en) |
JP (1) | JP2008525822A (en) |
WO (1) | WO2006071895A2 (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8993346B2 (en) | 2009-08-07 | 2015-03-31 | Nanomix, Inc. | Magnetic carbon nanotube based biodetection |
US9103775B2 (en) | 2002-01-16 | 2015-08-11 | Nanomix, Inc. | Nano-electronic sensors for chemical and biological analytes, including capacitance and bio-membrane devices |
US9291613B2 (en) | 2002-06-21 | 2016-03-22 | Nanomix, Inc. | Sensor having a thin-film inhibition layer |
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JP2008511008A (en) * | 2004-08-24 | 2008-04-10 | ナノミックス・インコーポレーテッド | Nanotube sensor device for DNA detection |
JP2009532064A (en) * | 2006-04-04 | 2009-09-10 | ナノミックス・インコーポレーテッド | Nanoelectronic detection of biomolecules using analyte amplification and reporter |
ES2289948B1 (en) * | 2006-07-19 | 2008-09-16 | Starlab Barcelona, S.L. | ELECTROPHYSIOLOGICAL SENSOR. |
JP5061342B2 (en) * | 2006-09-11 | 2012-10-31 | 国立大学法人大阪大学 | Carbon nanotube electrode and sensor using the electrode |
US8465634B2 (en) * | 2007-05-23 | 2013-06-18 | Arizona Board Of Regents | Systems and methods for integrated electrochemical and electrical detection |
WO2009026729A1 (en) * | 2007-08-30 | 2009-03-05 | University Of Waterloo | Amino acid pairing-based self assembling peptides and methods |
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GB0822733D0 (en) * | 2008-12-12 | 2009-01-21 | Univ Warwick | Nanotube electrochemistry |
JP2011220803A (en) * | 2010-04-08 | 2011-11-04 | Mitsumi Electric Co Ltd | Biosensor including field-effect transistor element |
US9184099B2 (en) | 2010-10-04 | 2015-11-10 | The Board Of Trustees Of The Leland Stanford Junior University | Biosensor devices, systems and methods therefor |
KR101963462B1 (en) | 2010-10-04 | 2019-03-28 | 제납시스 인크. | Systems and methods for automated reusable parallel biological reactions |
US9399217B2 (en) | 2010-10-04 | 2016-07-26 | Genapsys, Inc. | Chamber free nanoreactor system |
US9926596B2 (en) | 2011-05-27 | 2018-03-27 | Genapsys, Inc. | Systems and methods for genetic and biological analysis |
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US8585973B2 (en) | 2011-05-27 | 2013-11-19 | The Board Of Trustees Of The Leland Stanford Junior University | Nano-sensor array |
CN106591103B (en) | 2011-12-01 | 2021-06-04 | 吉纳普赛斯股份有限公司 | System and method for efficient electronic sequencing and detection |
CN104781658B (en) | 2012-10-16 | 2017-11-21 | 皇家飞利浦有限公司 | Integrated circuit, sensor device, measuring method and manufacture method with nano wire CHEMFET sensors |
CN102938397B (en) * | 2012-12-05 | 2015-09-09 | 苏州纳格光电科技有限公司 | Electronic device and preparation method thereof |
WO2014152625A1 (en) | 2013-03-15 | 2014-09-25 | Genapsys, Inc. | Systems and methods for biological analysis |
JP6473444B2 (en) * | 2013-05-29 | 2019-02-20 | シーエスアイアールCsir | Field effect transistor and gas detector including a plurality of field effect transistors |
US10125393B2 (en) | 2013-12-11 | 2018-11-13 | Genapsys, Inc. | Systems and methods for biological analysis and computation |
WO2015086654A1 (en) * | 2013-12-12 | 2015-06-18 | Altra Tech Limited | A capacitive sensor and method of use |
US9822401B2 (en) | 2014-04-18 | 2017-11-21 | Genapsys, Inc. | Methods and systems for nucleic acid amplification |
WO2016168734A1 (en) * | 2015-04-15 | 2016-10-20 | Malcolm Alastair J | Methods of manufacturing semiconductor arrays |
WO2017033922A1 (en) * | 2015-08-24 | 2017-03-02 | 国立大学法人 東京大学 | Method of detecting target substance using field-effect transistor |
CN116397014A (en) | 2016-07-20 | 2023-07-07 | 测序健康公司 | Systems and methods for nucleic acid sequencing |
EP3684951A4 (en) | 2017-09-21 | 2021-06-16 | Genapsys, Inc. | Systems and methods for nucleic acid sequencing |
US20190219512A1 (en) * | 2018-01-12 | 2019-07-18 | Clifford H. Kern, III | Device, procedure and system for detecting bacterial pathogens including methicillin-resistant staphylococcus aureus or clostridium difficile |
CN113588605A (en) * | 2021-06-18 | 2021-11-02 | 山东师范大学 | Method for detecting multiple environmental estrogens and application thereof |
US11845079B2 (en) * | 2021-12-08 | 2023-12-19 | International Business Machines Corporation | Integrated silicon platform for electronic biosensors |
Citations (4)
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---|---|---|---|---|
WO2001044796A1 (en) * | 1999-12-15 | 2001-06-21 | Board Of Trustees Of The Leland Stanford Junior University | Carbon nanotube devices |
US20020117659A1 (en) * | 2000-12-11 | 2002-08-29 | Lieber Charles M. | Nanosensors |
US20020179434A1 (en) * | 1998-08-14 | 2002-12-05 | The Board Of Trustees Of The Leland Stanford Junior University | Carbon nanotube devices |
US20040202603A1 (en) * | 1994-12-08 | 2004-10-14 | Hyperion Catalysis International, Inc. | Functionalized nanotubes |
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Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2003322653A (en) * | 2002-05-07 | 2003-11-14 | Toshiba Corp | Support and carrier for fixing probe |
AU2003291385A1 (en) * | 2002-11-08 | 2004-06-03 | Nanomix, Inc. | Nanotube-based electronic detection of biological molecules |
JP2004347532A (en) * | 2003-05-23 | 2004-12-09 | Japan Science & Technology Agency | Biosensor |
-
2005
- 2005-12-23 JP JP2007549560A patent/JP2008525822A/en active Pending
- 2005-12-23 WO PCT/US2005/047143 patent/WO2006071895A2/en active Application Filing
- 2005-12-23 EP EP05855662A patent/EP1831670A4/en not_active Withdrawn
Patent Citations (4)
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---|---|---|---|---|
US20040202603A1 (en) * | 1994-12-08 | 2004-10-14 | Hyperion Catalysis International, Inc. | Functionalized nanotubes |
US20020179434A1 (en) * | 1998-08-14 | 2002-12-05 | The Board Of Trustees Of The Leland Stanford Junior University | Carbon nanotube devices |
WO2001044796A1 (en) * | 1999-12-15 | 2001-06-21 | Board Of Trustees Of The Leland Stanford Junior University | Carbon nanotube devices |
US20020117659A1 (en) * | 2000-12-11 | 2002-08-29 | Lieber Charles M. | Nanosensors |
Non-Patent Citations (1)
Title |
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CUI ET AL.: "Nanowire Nanosensors for Hihgly Sensitive and Selective Detection of Biological and Chemical Species", SCIENCE, vol. 293, 17 August 2001 (2001-08-17), pages 1289 - 1291, XP002951584 * |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9103775B2 (en) | 2002-01-16 | 2015-08-11 | Nanomix, Inc. | Nano-electronic sensors for chemical and biological analytes, including capacitance and bio-membrane devices |
US9291613B2 (en) | 2002-06-21 | 2016-03-22 | Nanomix, Inc. | Sensor having a thin-film inhibition layer |
US8993346B2 (en) | 2009-08-07 | 2015-03-31 | Nanomix, Inc. | Magnetic carbon nanotube based biodetection |
Also Published As
Publication number | Publication date |
---|---|
WO2006071895A2 (en) | 2006-07-06 |
EP1831670A4 (en) | 2010-09-15 |
JP2008525822A (en) | 2008-07-17 |
EP1831670A2 (en) | 2007-09-12 |
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