CN115520954A - Application of microbial fuel cell in growth-promoting culture of prawns - Google Patents
Application of microbial fuel cell in growth-promoting culture of prawns Download PDFInfo
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Classifications
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/005—Combined electrochemical biological processes
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/04—Arrangements for treating water specially adapted to receptacles for live fish
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01M—PROCESSES OR MEANS, e.g. BATTERIES, FOR THE DIRECT CONVERSION OF CHEMICAL ENERGY INTO ELECTRICAL ENERGY
- H01M8/00—Fuel cells; Manufacture thereof
- H01M8/16—Biochemical fuel cells, i.e. cells in which microorganisms function as catalysts
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2203/00—Apparatus and plants for the biological treatment of water, waste water or sewage
- C02F2203/006—Apparatus and plants for the biological treatment of water, waste water or sewage details of construction, e.g. specially adapted seals, modules, connections
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E60/00—Enabling technologies; Technologies with a potential or indirect contribution to GHG emissions mitigation
- Y02E60/30—Hydrogen technology
- Y02E60/50—Fuel cells
Abstract
The invention relates to application of a microbial fuel cell in growth-promoting culture of prawns, belonging to the field of aquaculture. The microbial fuel cell not only can efficiently treat the aquaculture wastewater in situ, but also can promote the growth of aquatic animals.
Description
Technical Field
The invention belongs to the field of aquaculture, and particularly relates to application of a microbial fuel cell in growth-promoting culture of prawns.
Background
At present, the main variety of the prawn culture is Penaeus vannamei Boone, and the culture mode is mainly industrial circulating water culture. The industrial aquaculture mode belongs to an ultrahigh-density running water aquaculture mode, the aquaculture water consumption of the mode is large, the bait coefficient is high, a large amount of aquaculture wastewater is discharged to cause serious pollution to the surrounding environment, in addition, diseases are frequent in high-density aquaculture, the quality of seeds is degraded, and huge economic losses are caused to the aquaculture industry of the penaeus vannamei boone. Affecting the sustainable and healthy development of prawn culture.
The microbial fuel cell is a device for directly converting chemical energy in organic matters into electric energy by using microbes. The basic working principle is as follows: in the anaerobic environment of the anode chamber, organic matters are decomposed under the action of microorganisms to release electrons and protons, the electrons are effectively transferred between biological components and the anode by virtue of a suitable electron transfer mediator and are transferred to the cathode through an external circuit to form current, the protons are transferred to the cathode through a proton exchange membrane, and an oxidant (generally oxygen) obtains the electrons at the cathode and is reduced to be combined with the protons to form water. Microbial fuel cells are currently used for biological power generation while treating wastewater. However, no one in aquaculture finds the effect of the microbial fuel cell on the cultured organisms.
Disclosure of Invention
The invention aims to solve the technical problem of providing an application of a microbial fuel cell in growth promotion culture of prawns.
The invention is realized by the following technical scheme:
the microbial fuel cell comprises an anode chamber, a cathode chamber and an intermediate culture chamber, wherein an anion exchange membrane is arranged between the anode chamber and the intermediate culture chamber, a cation exchange membrane is arranged between the cathode chamber and the intermediate culture chamber for separation, the anode chamber is communicated with the intermediate culture chamber through a first communicating pipe, the intermediate culture chamber is communicated with the cathode chamber through a second communicating pipe, the anode chamber is communicated with the cathode chamber through a third communicating pipe, water in the cathode chamber can enter the intermediate culture chamber through the second communicating pipe, water in the intermediate culture chamber can enter the anode chamber through the first communicating pipe, water in the anode chamber can enter the cathode chamber through the third communicating pipe, mixed flora is arranged in the anode chamber, algae is arranged in the cathode chamber, a vent pipe is arranged between the anode chamber and the cathode chamber for communication, and an electrode in the anode chamber is connected with an electrode in the cathode chamber through an electric wire and a resistor;
the application method of the microbial fuel cell comprises the steps that water containing bait and excrement in the intermediate culture chamber is pumped into the anode chamber, the water is decomposed into small molecular nutrient substances under the action of anode bacteria, carbon dioxide is generated at the same time, bioelectricity generated in the decomposition process is conducted to the cathode electrode through the anode carbon rod and the electric wire, so that an electric field is generated between the anode chamber and the cathode chamber, and heavy metal ions in water in the intermediate culture chamber move to the cathode under the action of the electric field; the generated carbon dioxide enters the cathode chamber through the vent pipe, algae in the cathode chamber utilizes the carbon dioxide to perform respiration, the carbon dioxide is converted into organic matters in the cathode chamber, water in the anode chamber is retained for a period of time, macromolecular substances including protein are decomposed, the decomposed water is pumped into the cathode and is absorbed and utilized by the algae in the cathode chamber, and water rich in algae is pumped into the intermediate culture chamber from the cathode chamber and is used as biological bait, so that the uninterrupted microbial fuel cell closed circulating water culture system is formed.
Further, the microorganisms in the anode chamber are photosynthetic bacteria.
Furthermore, the algae in the cathode chamber are micro-Alternaria hainanensis and Nannochloropsis sp.
Compared with the prior art, the invention has the beneficial effects that:
the microbial fuel cell provided by the invention is used for treating organic matters in a culture water body and decomposing the organic matters into micromolecular peptides, one part of the micromolecular peptides enter a culture pond through an anion exchange membrane and are utilized by organisms in the culture pond, one part of the micromolecular peptides enter a cathode and are converted into algae biomass, the algae enters the culture pond as food, and current generated by the microbial fuel cell is transmitted between an anode chamber and a cathode chamber, so that an electric field is formed between the anode chamber and the cathode chamber, the electric field is favorable for improving immunity and growth of the aquatic organisms in the intermediate culture pond, metal ions in the culture water body move to the cathode chamber and are gathered in the cathode chamber, and heavy metals are removed in a precipitation form at the anode, so that the harm of the heavy metals in the water body to the aquatic organisms is avoided.
Drawings
FIG. 1 is a cross-sectional view of the application structure of the microbial fuel cell of the present invention in a culture pond. In the figure: 1. anode chamber, 2, cubic carbon electrode, 3, electric wire, 4, controller, 5, cathode chamber, 6, cathode electrode, 7, middle breed room, 8, water inlet system, 9, the water pump of intaking, 10, first communicating pipe, 11, second communicating pipe, 12, third communicating pipe, 13, breather pipe.
Detailed Description
The technical solution of the present invention is further explained by the following examples, but the scope of the present invention is not limited in any way by the examples.
Example 1
As shown in figure 1, the culture pond is rectangular, is externally connected with a water inlet system 8, a water inlet pump 9 provides water inlet power, two ends in the length direction are respectively separated into an anode chamber 1 and a cathode chamber 5 by an anion exchange membrane and a cation exchange membrane, the middle part is an intermediate culture chamber 7, the upper parts of the anode chamber 1 and the cathode chamber 5 are sealed, but a communicating air pipe 13, an electric wire 3 and a water circulation passage hole are reserved, the anode chamber 1 is provided with a block-shaped carbon electrode 2, the water body in the anode chamber 1 is inoculated with electrogenic photosynthetic bacteria (purchased from commercial products), the cathode chamber 5 is internally provided with a graphene oxide-CoAl layered double hydroxide composite electrode as a cathode electrode 6, the water body in the cathode chamber 5 is inoculated with algae including micro-streptococcum and nanum, the air pipe 13 is communicated with the cathode chamber 5 from the anode chamber 1, the conductive wire 3 is communicated with the graphene oxide-CoAl layered double hydroxide composite electrode of the cathode chamber 5 from the carbon electrode of the anode chamber 1 so as to facilitate the generated bioelectricity to be conducted from the anode chamber 1 to the cathode chamber 5, the controller 4 is arranged in the middle part
The anode chamber 1, the cathode chamber 5 and the middle culture pond are respectively provided with a water pump so as to pump water in the middle culture pond into the anode chamber 1 through a pump 10, the anode chamber 1 is communicated with the middle culture chamber 7 through a first communicating pipe 10, the middle culture chamber 7 is communicated with the cathode chamber 5 through a second communicating pipe 11, and the anode chamber 1 is communicated with the cathode chamber 5 through a third communicating pipe 12; after the water in the anode chamber 1 is treated by bacteria, one part of the water permeates into the intermediate culture pond through an anion exchange membrane, the other part of the water enters the cathode chamber 5 through a third communicating pipe 12, the water in the cathode chamber 5 enters the intermediate culture chamber 7 through a second communicating pipe 11 under the action of a water-like water pump, and the water in the intermediate culture chamber 7 enters the anode chamber 1 through a first communicating pipe 10 under the action of the water pump.
Feces, residual bait and the like in the aquaculture water body are decomposed into micromolecular nutrient substances under the action of photosynthetic bacteria in the anode chamber 1, carbon dioxide is generated at the same time, bioelectricity generated in the decomposition process is conducted to a cathode electrode through an anode carbon rod and a wire 3, so that an electric field is generated between the anode chamber 1 and the cathode chamber 5, and heavy metal ions in the intermediate aquaculture chamber 7 move to the cathode chamber 5; the generated carbon dioxide enters the cathode chamber 5 through the vent pipe 13, and the algae in the cathode chamber 5 uses the carbon dioxide to perform respiration to convert the carbon dioxide into organic matters; the generated biomass enters the culture room to be used as biological feed for culturing organisms.
The microbial fuel cell provided by the invention is used for treating organic matters in a culture water body and decomposing the organic matters into small molecular peptides, part of the small molecular peptides enter a culture pond through an anion exchange membrane and are utilized by organisms in the culture pond, part of the small molecular peptides enter a cathode and are converted into algae biomass, the algae serving as food enter the culture pond, and current generated by the microbial fuel cell is transmitted between an anode chamber 1 and a cathode chamber 5, so that an electric field is formed between the anode chamber 1 and the cathode chamber 5, the electric field is favorable for growth of aquatic organisms in a middle culture pond and improvement of immunity, disease drugs are reduced, metal ions in the culture water body are moved to the cathode chamber 5 by the electric field and are gathered in the middle cathode chamber 5, and the harm of heavy metals in the water body to aquatic organisms is avoided.
3 experimental groups are set in an application example, each group is provided with 3 parallel groups, and 600 litopenaeus vannamei (the average weight is 0.5 +/-0.02 g, and the body length is 3.2 +/-0.03 cm) is thrown in each culture pond. The experiment group 1 is a microbial fuel cell, the experiment group 2 is a normal circulating water culture group, the experiment group 3 is that weak electrodes are arranged at two ends of a culture pond, the culture pond is a rectangular culture pond with the length of 3 x 2m, the experiment group 1 and the experiment group 3 have the same structure, and the difference lies in that photosynthetic bacteria and algae are not arranged at two ends of the experiment group 3, namely, biological electricity can not be generated by decomposing organic matters through microorganisms, only an external artificial power supply is used for providing direct current voltage of 0.2-0.5V, and a weak electric field is formed in the culture pond.
After 60d of cultivation, the effects are as follows:
TABLE 1 comparison of growth Performance of three groups of Penaeus vannamei Boone
| Index (I) | Experimental group 1 | Experimental group 2 | Experimental group 3 |
| Survival rate (%) | 94.67±0.28 | 90.40±0.21 | 91.82±0.13 |
| Terminal average body weight (g) | 9.9±0.05 | 8.8±0.04 | 9.1±0.04 |
| Terminal average body length (cm) | 8.9±0.06 | 8.4±0.04 | 8.6±0.03 |
The results show that: the survival rates of all groups are obviously different, and the experimental group 1 is obviously higher than those of the other two groups; the average final body weight of the experimental group 2 and the experimental group 3 has no significant difference, but is significantly lower than that of the experimental group 1; the average body length at the end of the experimental group 1 and the experimental group 3 has no significant difference, but is significantly higher than that of the experimental group 2.
TABLE 2 comparison of digestive tract digestive enzyme activities of three groups of Penaeus vannamei Boone
| Index (es) | Experimental group 1 | Experimental group 2 | Experimental group 3 |
| Protease (U/(mg. Pro)) | 59.26±1.03 | 41.25±0.68 | 47.35±0.46 |
| Amylase (U/(mg.pro)) | 1.91±0.09 | 1.45±0.18 | 1.52±0.07 |
| Lipase (U/(mg. Pro)) | 11.59±0.72 | 6.12±0.56 | 8.24±0.82 |
The results show that: the digestive enzyme activities of all groups have significant difference, and the digestive enzyme activities of the experimental group 1 are significantly higher than those of the other two groups.
TABLE 3 comparison of the nonspecific immunoenzyme activity of three groups of Penaeus vannamei Boone sera
The results show that: the activity of the phenol oxidizing enzyme of the experimental group 1 and the experimental group 3 is not obviously different, but is obviously higher than that of the experimental group 2; the activity of the superoxide dismutase in the experimental group 2 is obviously higher than that of the superoxide dismutase in the other two groups; the lysozyme activities of the experimental group 2 and the experimental group 3 have no significant difference, but are significantly lower than that of the experimental group 1.
Claims (3)
1. The microbial fuel cell is applied to growth-promoting culture of prawns and is characterized by comprising an anode chamber, a cathode chamber and an intermediate culture chamber, wherein an anion exchange membrane is arranged between the anode chamber and the intermediate culture chamber, a cation exchange membrane is arranged between the cathode chamber and the intermediate culture chamber for separation, the anode chamber is communicated with the intermediate culture chamber through a first communicating pipe, the intermediate culture chamber is communicated with the cathode chamber through a second communicating pipe, the anode chamber is communicated with the cathode chamber through a third communicating pipe, water in the cathode chamber can enter the intermediate culture chamber through the second communicating pipe, water in the intermediate culture chamber can enter the anode chamber through the first communicating pipe, water in the anode chamber can enter the cathode chamber through the third communicating pipe, mixed flora is arranged in the anode chamber, algae are arranged in the cathode chamber, a vent pipe is communicated between the anode chamber and the cathode chamber, and an electrode in the anode chamber are connected through an electric wire and a resistor;
the application method of the microbial fuel cell comprises the steps that water containing bait and excrement in the intermediate culture chamber is pumped into the anode chamber, the water is decomposed into small molecular nutrient substances under the action of anode bacteria, carbon dioxide is generated at the same time, bioelectricity generated in the decomposition process is conducted to the cathode electrode through the anode carbon rod and the electric wire, so that an electric field is generated between the anode chamber and the cathode chamber, and heavy metal ions in water in the intermediate culture chamber move to the cathode under the action of the electric field; the generated carbon dioxide enters the cathode chamber through the vent pipe, algae in the cathode chamber perform photosynthesis by using the carbon dioxide, the carbon dioxide is converted into organic matters in the cathode chamber, water in the anode chamber is retained for a period of time, macromolecular substances including protein are decomposed, the decomposed water is pumped into the cathode and is absorbed and utilized by the algae in the cathode chamber, and the water rich in algae is pumped into the intermediate culture chamber from the cathode chamber and is used as biological bait, so that the uninterrupted microbial fuel cell closed circulating water culture system is formed.
2. The use of a microbial fuel cell according to claim 1 in growth-promoting aquaculture of prawns, wherein the microbes in the anode compartment comprise Proteobacteria, actinobacilla, firmicutes, bacteroides, chloroflexi, saccharomyces, tenericutes, synergestites, spirochaetes.
3. The use of the microbial fuel cell of claim 1 in growth-promoting culture of prawns, wherein the algae in the cathode chamber are thalassiosira microscopica and nannochloropsis.
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