CN109916782A - A method of it is detected using miillpore filter - Google Patents
A method of it is detected using miillpore filter Download PDFInfo
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- CN109916782A CN109916782A CN201910188816.4A CN201910188816A CN109916782A CN 109916782 A CN109916782 A CN 109916782A CN 201910188816 A CN201910188816 A CN 201910188816A CN 109916782 A CN109916782 A CN 109916782A
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- miillpore filter
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Abstract
The invention discloses a kind of methods detected using miillpore filter to be added to pad pasting liquid on glass slide before the ingredient that detection miillpore filter is retained, then miillpore filter is placed on pad pasting liquid, so that miillpore filter dissolves；Miillpore filter after the completion of preparation forms colorless and transparent homogeneous body.The present invention provides a kind of method detected using miillpore filter, can dissolve miillpore filter, in favor of observing, detecting the cell or other molecules on miillpore filter.
Description
Technical field
The present invention relates to medical detection field, specially a kind of method detected using miillpore filter.
Background technique
When carrying out micro- sem observation to cell or other molecules, in order to smoothly observe these under the microscope
Fine particle, often requires to use oil mirror and amplification factor needs are even higher at 1000 times.At this time if miillpore filter is too thick,
Often layering is serious in microscopy for object, result judgement is not easy to, so the miillpore filter thickness used is often micro- in 20-50
Within rice.The existing method such as Chinese Patent Application No. detected using miillpore filter: disclosed in 201110462479.7
A kind of detection method and film transparent liquid using UF membrane, in the forming process that is retained of detection miillpore filter, using film
Transparent liquid is added on filter membrane, so that the filter membrane shows transparent state；This method it is practical but still
Have deficiency, need to wait for miillpore filter when such as detecting and be completely dried, remain on another aspect miillpore filter other electrolyte at
It is transparent not exclusively that timesharing will lead to miillpore filter, so still having room for improvement.
Summary of the invention
The present invention provides a kind of method detected using miillpore filter, can dissolve miillpore filter, in favor of seeing
It examines, detect cell or other molecules on miillpore filter.
In order to achieve the above object, the technical solution adopted by the present invention is that: it, will before the ingredient that detection miillpore filter is retained
Pad pasting liquid is added on glass slide, and then miillpore filter is placed on pad pasting liquid, so that miillpore filter dissolves；After the completion of preparation
Miillpore filter form colorless and transparent homogeneous body.
Further, the material of the miillpore filter is Kynoar (PVDF), with a thickness of 20 microns.
Further, the pad pasting liquid material is the organic solvent of high concentration.
Further, the organic solvent is dimethylformamide or dimethyl acetamide.
Beneficial effects of the present invention: pad pasting liquid is water-soluble and can quickly dissolve miillpore filter, after miillpore filter dissolution
Colorless and transparent homogeneous body is formed, convenient for insoluble to the cell or other molecules retained on miillpore filter etc. under the microscope
The visible component of solution is detected, and is avoided miillpore filter opaque and is influenced detection effect, while complete without waiting for miillpore filter
White drying can be observed, be detected, and reduce the waiting time to improve detection efficiency.
1, miillpore filter is dissolved as colorless and transparent liquid under the action of pad pasting liquid, and coverslip lid is closed on glass slide
When, it can be ensured that coverslip can press very flat, it is ensured that detection quality.
Specific embodiment
In order to make those skilled in the art more fully understand technical solution of the present invention, the present invention is retouched in detail below
State, the description of this part be only it is exemplary and explanatory, should not have any restriction effect to protection scope of the present invention.
It is of the invention the specific scheme is that pad pasting liquid is added to glass slide before the ingredient that detection miillpore filter is retained
On, then miillpore filter is placed on pad pasting liquid, so that miillpore filter dissolves；Miillpore filter after the completion of preparation forms colourless
Clear homogeneous body.
Preferably, the material of the miillpore filter is Kynoar (PVDF), with a thickness of 20 microns.
Preferably, the pad pasting liquid material is the organic solvent of high concentration.
Preferably, the organic solvent is dimethylformamide or dimethyl acetamide.
Specific embodiment: will be mounted in specimen collecting device using miillpore filter made of Kynoar (PVDF),
Then the sample in collection device is collected and the cell or other molecules that are adhered on miillpore filter is contaminated
Color, cleaning and etc., another Chinese invention patent of the applicant, Patent No. can be used in specific steps
Other methods can also be used in technical solution described in CN201010529398.X；Dyeing, cleaning and etc. after the completion of, carry glass
The pad pasting liquid made of dimethylformamide or dimethyl acetamide is added dropwise on piece, then filters the micropore in specimen collecting device
Film is removed and placed to be covered on coverslip micro- after pad pasting liquid dissolves miillpore filter in the pad pasting liquid zone domain on glass slide
The film-making of sample is completed on the lysigenous region of hole filter membrane.When detection, by sample be placed under microscope with to cell or its
The undissolved visible component such as its molecule is detected.
Above to it is provided by the present invention it is a kind of be described in detail using the method that miillpore filter is detected, this
Principle and implementation of the present invention are described in text, the present invention that the above embodiments are only used to help understand
Method with its core concept；At the same time, for those skilled in the art is being embodied according to the thought of the present invention
There will be changes in mode and application range, in conclusion the content of this specification should not be construed as to limit of the invention
System.
Claims (4)
1. a kind of method detected using miillpore filter, which is characterized in that before the ingredient that detection miillpore filter is retained,
Pad pasting liquid is added on glass slide, then miillpore filter is placed on pad pasting liquid, so that miillpore filter dissolves；Preparation is completed
Miillpore filter afterwards forms colorless and transparent homogeneous body.
2. a kind of method detected using miillpore filter according to claim 1, which is characterized in that the micropore filter
The material of film is Kynoar, with a thickness of 20 microns.
3. a kind of method detected using miillpore filter according to claim 1, which is characterized in that the pad pasting liquid
Material is the organic solvent of high concentration.
4. a kind of method detected using miillpore filter according to claim 3, which is characterized in that described organic molten
Agent is dimethylformamide or dimethyl acetamide.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910188816.4A CN109916782A (en) | 2019-03-13 | 2019-03-13 | A method of it is detected using miillpore filter |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910188816.4A CN109916782A (en) | 2019-03-13 | 2019-03-13 | A method of it is detected using miillpore filter |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109916782A true CN109916782A (en) | 2019-06-21 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910188816.4A Pending CN109916782A (en) | 2019-03-13 | 2019-03-13 | A method of it is detected using miillpore filter |
Country Status (1)
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Application publication date: 20190621 |