CN109142491A - Water quality monitoring method based on continuous flow without film biological-cathode microbiological fuel cell - Google Patents
Water quality monitoring method based on continuous flow without film biological-cathode microbiological fuel cell Download PDFInfo
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- CN109142491A CN109142491A CN201810977523.XA CN201810977523A CN109142491A CN 109142491 A CN109142491 A CN 109142491A CN 201810977523 A CN201810977523 A CN 201810977523A CN 109142491 A CN109142491 A CN 109142491A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/403—Cells and electrode assemblies
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- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01M—PROCESSES OR MEANS, e.g. BATTERIES, FOR THE DIRECT CONVERSION OF CHEMICAL ENERGY INTO ELECTRICAL ENERGY
- H01M8/00—Fuel cells; Manufacture thereof
- H01M8/16—Biochemical fuel cells, i.e. cells in which microorganisms function as catalysts
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E60/00—Enabling technologies; Technologies with a potential or indirect contribution to GHG emissions mitigation
- Y02E60/30—Hydrogen technology
- Y02E60/50—Fuel cells
Abstract
The invention discloses a kind of water quality monitoring method based on continuous flow without film biological-cathode microbiological fuel cell, the microbiological fuel cell includes anode detection unit, cathode detection unit and anode and cathode communicating pipe；Water sample is after pretreatment, it is introduced into anode detection unit of the continuous flow without film biological-cathode microbiological fuel cell, then cathode detection unit is entered by anode and cathode communicating pipe, finally flowed out from cathode detection unit, by analyzing output electric current of the continuous flow without film biological-cathode microbiological fuel cell for being passed through pretreated water sample to be measured and standard water sample in real time, the water quality situation of water sample to be measured is judged.This monitoring method can effectively monitor single toxicity pollution, organic matter overloads and the multiple-quality waters abnormal conditions such as toxic pollutant-is organic compound contaminated, expand the application range of the water quality monitoring method based on microbiological fuel cell.
Description
Technical field
The invention belongs to water quality biological Toxicity Monitoring technical fields, and in particular to one kind is based on continuous flow without film biological-cathode
The water quality monitoring method of microbiological fuel cell.
Background technique
With China's process of industrialization it is continuous propulsion and rapid development of economy, various chemical products it is a large amount of using with
The development & construction behavior of the mankind causes serious pollution to water body environment.On-line water quality monitoring method is developed, is realized to water body
The early warning of pollution advantageously reduces the economic loss that sudden water pollution event causes, and ensures ecological safety.It is existing
The testing index of monitoring water quality on line system is mainly the physical and chemical indexes such as COD, pH, conductivity, although these indexs can
To assess change of water quality, but it can not reflect the bio-toxicity and comprehensive toxicity of water body.Aqueous bio toxicity is monitored on-line, to evaluation
Contamination accident is significant to risk, the comprehensive assessment water pollution situation of human health.
Monitoring method based on microbiological fuel cell (microbial fuel cell, MFC) is water quality biological toxicity prison
One new and developing branch of survey technology.Its principle for monitoring water quality is the metabolism that toxic pollutant will affect electro-chemical activity microorganism
The real time monitoring of water quality toxicity can be realized by monitoring MFC output signal so as to cause the variation of MFC output signal in activity.
Compared to using fish, flea class etc. as biological subject, the monitoring method based on MFC does not need additional signal transduction device, can
To realize from driving, it is more suitable for continuous on-line analysis.
The existing monitoring method based on MFC, mainly using biological anode as sensing element, when monitoring single toxicity pollution
With preferable sensitivity.However, toxic pollutant and organic matter overload simultaneous combined pollution in practical water pollution
Occupy sizable ratio.Using the anode of MFC as sensing element when monitoring such pollution the defect with principle, that is, have
The increase of machine object concentration can improve the metabolic activity of electro-chemical activity microorganism, weaken toxic pollutant and output signal is caused to decline
Amplitude, cause early warning to fail.How to realize effective monitoring to combined pollution, is the water quality biological Toxicity Monitoring based on MFC
The major issue that technology faces.
Summary of the invention
In view of the above-mentioned problems, the present invention provides a kind of water based on continuous flow without film biological-cathode microbiological fuel cell
Matter monitoring method.Compared with tradition is based on the method for microbiological fuel cell, this method is characterized in raw without film using continuous flow
Object cathode microbial fuel cell, and with biological anode and biological-cathode collectively as detecting element, water sample to be measured is introduced into sun
Then pole detection unit enters cathode detection unit by anode and cathode communicating pipe, by real-time monitoring continuous flow without film biology yin
The output electric current of atomic biological fuel cell, and be passed through the continuous flow of standard water sample without film biological-cathode microbiological fuel cell
Output signal comparison, realize organic compound contaminated etc. more to single toxicity pollution, organic matter overload and toxic pollutant-
The monitoring of kind water quality abnormal conditions.Present invention firstly provides continuous flow is applied to water without film biological-cathode microbiological fuel cell
Field is surveyed in quality supervision, and expands the monitoring range of the water quality monitoring method based on microbiological fuel cell, particular content of the present invention
It is as follows:
A kind of continuous flow without film biological-cathode microbiological fuel cell, including anode detection unit, cathode detection unit with
And anode and cathode communicating pipe.The inside of the anode detection unit and cathode detection unit is cylindrical cavity, and anode detection is single
First cavity volume is 14-56mL, and cathode detection unit cavity volume is 14-112mL.Cavity inside is placed with carbon cloth electrode, carbon
Cloth electrode is connect after being drawn by titanium silk from top electrodes hole with control unit.The covering of anode carbon cloth electrode surface has electrochemistry
Active microorganism composition anaerobic organism film, and cathode carbon cloth electrode surface covering have electro-chemical activity microorganism group at it is good
Oxygen biomembrane.Cathode detection unit side wall is equipped with solarization air cap, for being inserted into the aerator connecting with air pump.Anode detection unit
Inlet and outlet are equipped with cathode detection unit side wall, run it with continuous stream mode.Anode and cathode communicating pipe is direct
The anode water outlet of jointed anode detection unit and the cathode water inlet of cathode detection unit, connection bore are 2-10mm, pipe
A length of 20-50mm.In above-mentioned size range, it can effectively inhibit oxygen from cathode detection unit to anode anode and cathode communicating pipe
Detection unit diffusion guarantees that continuous flow has stable output electric current without film biological-cathode microbiological fuel cell.
A kind of water quality monitoring method based on above-mentioned continuous flow without film biological-cathode microbiological fuel cell, testing process is such as
Shown in Fig. 1.The method uses two groups of identical continuous flows with above-mentioned configuration without film biological-cathode microbiological fuel
Battery I and II is detected, wherein continuous flow without film biological-cathode microbiological fuel cell I be continually fed into it is pretreated to
Water sample is surveyed, continuous flow is continually fed into pretreated standard water sample, standard water sample without film biological-cathode microbiological fuel cell II
It is no more than the pure water of 0.001S/m for conductivity.Above-mentioned preprocessing process, including making the dissolved oxygen in water sample by exposing nitrogen
Concentration is less than 0.5mg/L, makes water sample temperature within the scope of 25.0 ± 1.0 DEG C by heating or freezing, and is made by adding sodium chloride
Water sample conductivity is within the scope of 1.0 ± 0.1S/m, and 0.062g/L-0.246g/L sodium acetate is added into water sample.Water sample warp
After crossing pretreatment, liquid feeding pump is first passed through from anode water inlet and flows into anode of the continuous flow without film biological-cathode microbiological fuel cell
Then detection unit enters cathode detection unit by anode and cathode communicating pipe, finally flow out from cathode detection unit.Water sample feed liquor
Flow velocity is 0.5-4mL/min, detection time 15-60min.In above parameter range, water sample can be with electrode biomembrane
It comes into full contact with, the change of water quality of water sample to be measured can be quickly reflected in the variation of microbiological fuel cell output electric current.It is above-mentioned
In detection process, control unit test constantly simultaneously analyzes two groups of continuous flows without film biological-cathode microbiological fuel cell I and II
Electric current is exported, judges whether the water quality of water sample to be measured is abnormal.
Control unit described in above method can be applied to continuous flow without film biological-cathode microbiological fuel cell I and II
Add the voltage of 0-1V, control precision is not less than 1mV；Continuous flow can be measured without film biological-cathode microbiological fuel cell I simultaneously
With II output electric current, measurement accuracy is not less than 1 μ A, and measurement frequency is not less than 1Hz.When detection, control unit is according to formula (1)
Real-time calculating current variation coefficient R judges whether the water quality of water sample to be measured is abnormal:
R=| (I1-I2)/I2| × 100% (1)
I1For output electric current of the continuous flow without film biological-cathode microbiological fuel cell I for being continually fed into water sample to be measured, I2For
It is continually fed into output electric current of the continuous flow without film biological-cathode microbiological fuel cell II of standard water sample, I1And I2It is detection
Real-time current per minute in the process determines that water quality is abnormal, otherwise water quality is normal as R > 20.
When there is excessive organic matter, i.e. generation organic matter overload in water sample to be measured, since organic matter is anode electrochemical
The metabolism substrate of active microorganism, organic concentration raising can promote its metabolic activity, when its metabolic activity has been in saturation shape
When state, anode microorganism can not be metabolized excessive organic matter, cause in anode water outlet containing the organic matters not being metabolized largely.It crosses
After the organic matter of amount enters cathode detection unit with anode water outlet, organic matter can compete with the electronics of cathode electrode plate and be used as cathode
The electron donor of electro-chemical activity microorganism declines it using the rate of cathode electrode plate electronics.Cathode detection unit simultaneously
In be likely present some non-electroactive microorganisms, these microorganisms can use organic matter and carry out aerobic heterotrophism generation
It thanks, competes dissolved oxygen with electro-chemical activity microorganism, be also unfavorable for cathode reaction progress.Since excessive organic matter causes cathode
Reaction rate reduces, and is passed through output electric current I of the continuous flow without film biological-cathode microbiological fuel cell I of water sample to be measured1It can not
Disconnected decline, therefore the monitoring to water sample organic matter overload event to be measured may be implemented in method of the invention.
When single toxicity pollution occurs for water sample to be measured, the metabolism of the electro-chemical activity microorganism in anode detection unit is living
Property can be inhibited by toxic pollutant, generate electronics rate decline, while its be metabolized organic matter rate also reduce, make anode
There may be the organic matters not being metabolized in water outlet.The anode for not being metabolized containing toxic pollutant and organic matter is discharged again through yin
After anode communicating pipe flows into cathode detection unit, electro-chemical activity microorganism in cathode detection unit can be caused to utilize the energy of electronics
Power decline.Since anode and cathode reaction rate reduces, the continuous flow of water sample to be measured is passed through without film biological-cathode microbiological fuel electricity
The output electric current I in pond I1Can constantly it decline, therefore the monitoring to single toxicity contamination accident may be implemented in method of the invention.
When toxic pollutant occurs for water sample to be measured and organic matter overloads simultaneous combined pollution, organic concentration
Increase the activity that the impact with toxic pollutant respectively facilitated and inhibited electro-chemical activity microorganism in anode detection unit, anode
Toxic pollutant and excessive organic matter can be contained in water outlet.Anode water outlet flows into cathode detection unit through anode and cathode communicating pipe again
Afterwards, toxic pollutant and excessive organic matter can cause the reduction of cathode reaction rate.Since combined pollution causes cathode reaction
Rate reduces, and is passed through output electric current I of the continuous flow without film biological-cathode microbiological fuel cell I of water sample to be measured1Under meeting constantly
Drop, therefore the monitoring to the organic compound contaminated event of toxic pollutant-may be implemented in method of the invention.
Compared with the existing water quality monitoring method based on microbiological fuel cell, the present invention, which has not only expanded, can monitor water body
The range of pollution, advantage also reside in using the electro-chemical activity microorganism using pole plate electronics instead of the noble metals such as platinum as
Cathod catalyst.Since microorganism has the characteristics that continuous growth and breeding, using biocatalyst can to avoid catalyst poisoning,
After long-play the problems such as reduced performance, it is conducive to extend the service life of microbiological fuel cell, while reducing and being built into
This.It is also an advantage of the present invention that with anode and cathode communicating pipe instead of the amberplex of higher cost.Traditional proton cross-film
It transports (cation-exchange membrane or proton exchange membrane) and often causes anode and cathode since permeability of the membrane material to proton is limited
Liquid proton concentration differs greatly, and is unfavorable for maintaining the metabolic activity of electro-chemical activity biomembrane, electricity generation performance meeting after longtime running
It constantly reduces, influences service life.And pass through anode and cathode communicating pipe jointed anode detection unit water outlet and cathode in the present invention
Detection unit water inlet, anode water outlet can flow directly into cathode detection unit, reach proton with water flow free diffusing
Cathode avoids transmission limitation caused by diaphragm, is conducive to extend the service life of microbiological fuel cell.Even if in water to be measured
When the buffer salinity of sample is lower, due to the continuous flow assurance transmission of proton of water flow, continuous flow provided by the invention without
Film biological-cathode microbiological fuel cell remains to maintain the pH balance of anode and cathode detection unit, keeps preferable electricity generation performance.
Detailed description of the invention
Attached drawing 1 is the testing process schematic diagram of water quality monitoring method provided by the invention.
Attached drawing 2 is schematic diagram of the continuous flow provided by the invention without film biological-cathode microbiological fuel cell I.
Attached drawing 3 is output current curve of the continuous flow provided by the invention without film biological-cathode microbiological fuel cell I.
Attached drawing 4 is the output curent change figure of method monitoring single toxicity contamination accident provided by the invention.
Attached drawing 5 is the output curent change figure of method monitoring organic matter overload event provided by the invention.
Attached drawing 6 is that the output electric current of the method monitoring organic compound contaminated event of toxic pollutant-provided by the invention becomes
Change figure.
Description of symbols: in attached drawing 2,1 is continuous flow without film biological-cathode microbiological fuel cell I, and 2 be anode detection
Unit, 3 be anode water inlet, and 4 be anode carbon cloth electrode, and 5 be the titanium silk of jointed anode carbon cloth electrode 4 and control unit 17, and 6 are
Anode electrode hole, 7 be anode water outlet, and 8 be anode and cathode communicating pipe, and 9 be cathode detection unit, and 10 be connection cathode carbon cloth electrode
14 and control unit 17 titanium silk, 11 be solarization air cap, and 12 be cathode electrode hole, and 13 be cathode water outlet, and 14 be cathode carbon cloth electricity
Pole, 15 be aerator, and 16 be cathode water inlet, and 17 be control unit.
Specific embodiment
The present invention is described further below by way of specific embodiment.It should be pointed out that specific implementation of the invention
Mode is not limited to embodiments given below, and the technical solution based on the content of present invention should be also included in of the presently claimed invention
Protection scope.
Embodiment 1
One provided by the invention composition of the specific continuous flow without film biological-cathode microbiological fuel cell such as Fig. 2 institute
Show, which includes anode detection unit 2, cathode detection unit 9 and yin-yang without film biological-cathode microbiological fuel cell 1
Pole communicating pipe 8.
The shape of anode detection unit 2 is cuboid, and inside is cylindrical cavity, cavity diameter 3cm, high 4cm, cavity
Volume is 28mL, and cavity inside is placed with anode carbon cloth electrode 4, electrode surface covering have electro-chemical activity microorganism group at
Anaerobic organism film is equipped with anode electrode hole 6 at the top of anode detection unit 2, and anode carbon cloth electrode 4 is by the extraction of titanium silk 5, with control
Unit 17 connects.2 side wall of anode detection unit is respectively equipped with anode water inlet 3 and anode water outlet 7, anode water inlet 3 and one
Peristaltic pump connection, runs anode detection unit 2 with continuous stream mode.
The shape of cathode detection unit 9 is cuboid, and inside is cylindrical cavity, cavity diameter 3cm, high 8cm, cavity
Volume is 56mL, and cavity inside is placed with cathode carbon cloth electrode 14, electrode surface covering have electro-chemical activity microorganism group at
Aerobic biologic membrane, be equipped with cathode electrode hole 12 at the top of cathode detection unit 9, cathode carbon cloth electrode 14 by the extraction of titanium silk 10,
It is connect with control unit 17.9 side wall of cathode detection unit is equipped with solarization air cap 11, for being inserted into the aerator connecting with air pump
15,9 side wall of cathode detection unit is additionally provided with cathode water inlet 16 and cathode water outlet 13, makes cathode detection unit 9 with continuous
Stream mode is run.
Anode detection unit 2 and cathode detection unit 9 are connected by anode and cathode communicating pipe 8.Communicating pipe one end jointed anode
The anode water outlet 7 of detection unit 2, the other end connect the cathode water inlet 16 of cathode detection unit 9.Connection bore is 5mm,
Pipe range is 50mm.
Control unit 17 is a potentiostat, is applied to above-mentioned continuous flow without film biological-cathode microbiological fuel cell I
The constant voltage of 0.042V.
When operation, water sample flows into anode detection unit 2 from anode water inlet 3, the electro-chemical activity of anode carbon cloth electrode 4
Biomembrane can be metabolized organic matter, while generate proton and electronics.After water sample and anode biofilm contact, from anode detection unit 2
Anode water outlet 7 flow out, then by anode and cathode communicating pipe 8 from cathode water inlet 16 flow into cathode detection unit 9.Cathode carbon
The electro-chemical activity biomembrane of cloth electrode 14 receives the electronics transmitted through external circuit, and oxygen reduction occurs for Catalytic Proton and dissolved oxygen
Electricity generation process is completed in reaction.As shown in figure 3, having when being passed through in water sample of the continuous flow without film biological-cathode microbiological fuel cell I
When machine object constant concentration and non-toxic pollutant, output electric current keeps stablizing.
Embodiment 2
Fig. 4 is combined in this example, illustrates the output electric current using method provided by the invention monitoring single toxicity contamination accident
Variation.
Sodium acetate additive amount is 0.082g/L in pre-treatment step, is free of organic matter before water sample processing to be measured, therefore pass through
After pretreatment, with sodium acetate for single organic matter in standard water sample and water sample to be measured, concentration 0.082g/L.To water to be measured
Heavy metal Hg is added in sample, makes Hg2+Final concentration of 1.5mg/L simulates single toxicity contamination accident.Water sample feed liquor flow velocity is set as
2mL/min, detection time 30min.
As shown in figure 4, in the detection process, being passed through the continuous flow of standard water sample without film biological-cathode microbiological fuel cell
II output electric current I2All-the-time stable is in 45.2 ± 0.1 μ A, and the output electric current I of microbiological fuel cell I1It is being passed through water to be measured
Start to continue reduction after sample 5min.Due to the output electric current I of microbiological fuel cell II2It keeps stablizing, therefore curent change system
Number R is with I1Reduction and constantly increase.After being passed through water sample 8min to be measured, R is more than 20, determines that water quality is abnormal.This result is said
Bright water quality monitoring method provided by the invention can delicately monitor single toxicity contamination accident.
Embodiment 3
Fig. 5 is combined in this example, illustrates to become using the output electric current of method provided by the invention monitoring organic matter overload event
Change.
Sodium acetate additive amount is 0.082g/L in pre-treatment step, has contained 0.082g/L acetic acid before water sample processing to be measured
Sodium, therefore after pretreatment, the sodium acetate concentration in standard water sample and water sample to be measured is respectively 0.082g/L and 0.164g/L.
Water sample feed liquor flow velocity is set as 2mL/min, detection time 30min.As shown in figure 5, the output electric current of microbiological fuel cell I
I1It can be substantially reduced after being passed through water sample to be measured, and the output electric current I of microbiological fuel cell II2It keeps stablizing.Curent change system
The value of number R is with I1Decline and increase, more than 20 after being passed through water sample 18min to be measured, determine that water quality is abnormal.The result shows
Water quality monitoring method provided by the invention can delicately monitor organic matter overload event.
Embodiment 4
Fig. 6 is combined in this example, illustrates to monitor the organic compound contaminated thing of toxic pollutant-using method provided by the invention
The output curent change of part.
Sodium acetate additive amount is 0.082g/L in pre-treatment step, has contained 0.082g/L acetic acid before water sample processing to be measured
Sodium, therefore after pretreatment, the sodium acetate concentration in standard water sample and water sample to be measured is respectively 0.082g/L and 0.164g/L.
Heavy metal Hg is added into water sample to be measured simultaneously, makes Hg2+Final concentration of 1.5mg/L simulates the compound dirt of toxic pollutant-organic matter
Dye event.Water sample feed liquor flow velocity is set as 2mL/min, detection time 30min.From fig. 6 it can be seen that Microbial fuel is electric
The output electric current I in pond II2It keeps stablizing, and the output electric current I of microbiological fuel cell I1Start after being passed through water sample 5min to be measured
It reduces rapidly, the value of curent change coefficients R, more than 20, determines that water quality is abnormal after being passed through water sample 9min to be measured.In addition, monitoring
When combined pollution event, the electric current fall of microbiological fuel cell I be significantly greater than its same concentration single toxicity is polluted or
The response of organic matter overload event, after being passed through water sample 20min to be measured, the output electric current I of microbiological fuel cell I1It has reached
New stable state is arrived.Have the result shows water quality monitoring method provided by the invention can delicately monitor toxic pollutant-
Machine object combined pollution event.
Claims (7)
1. the water quality monitoring method based on continuous flow without film biological-cathode microbiological fuel cell, it is characterised in that: the method
It is detected using two groups of identical continuous flows without film biological-cathode microbiological fuel cell I (1) and II, wherein continuously
Stream is continually fed into pretreated water sample to be measured without film biological-cathode microbiological fuel cell I (1), and continuous flow is without film biological-cathode
Microbiological fuel cell II is continually fed into pretreated standard water sample, and standard water sample is that conductivity is pure no more than 0.001S/m
Water purification, water sample feed liquor flow velocity are 0.5-4mL/min, detection time 15-60min, and control unit (17) real-time measurement is simultaneously point
Analysis is passed through the continuous flow of pretreated water sample to be measured and standard water sample without film biological-cathode microbiological fuel cell I (1) and II
Output electric current, judge whether the water quality of water sample to be measured abnormal.
2. pretreatment described in claim 1 is led to including making the dissolved oxygen concentration in water sample be less than 0.5mg/L by exposing nitrogen
Crossing heating or refrigeration makes water sample temperature within the scope of 25.0 ± 1.0 DEG C, by add sodium chloride make water sample conductivity 1.0 ±
Within the scope of 0.1S/m, and 0.062g/L-0.246g/L sodium acetate is added into water sample.
3. continuous flow described in claim 1 is without film biological-cathode microbiological fuel cell I (1), it is characterised in that: described continuous
Stream includes that anode detection unit (2), cathode detection unit (9) and anode and cathode connect without film biological-cathode microbiological fuel cell I (1)
Siphunculus (8), when detection, water sample is introduced into continuous flow without film biological-cathode microbiological fuel cell I (1) after pretreatment
Anode detection unit (2) then enters cathode detection unit (9) by anode and cathode communicating pipe (8), finally from cathode detection unit
(9) it flows out.
4. anode detection unit (2) as claimed in claim 3, it is characterised in that the inside of the anode detection unit (2) is circle
Cylindrical cavity, cavity volume 14-56mL, cavity inside are placed with anode carbon cloth electrode (4), and electrode surface covering has electrification
The anaerobic organism film of active microorganism composition is learned, is equipped with anode electrode hole (6) at the top of anode detection unit (2), anode carbon cloth electricity
Pole (4) is drawn by titanium silk (5) and is connect with control unit (17), and anode detection unit (2) side wall is respectively equipped with anode water inlet
(3) it is connect with anode water outlet (7), anode water inlet (3) with liquid feeding pump, transports anode detection unit (2) with continuous stream mode
Row.
5. cathode detection unit (9) as claimed in claim 3, it is characterised in that the inside of the cathode detection unit (9) is circle
Cylindrical cavity, cavity volume 14-112mL, cavity inside are placed with cathode carbon cloth electrode (14), and electrode surface covering has electricity
Chemical activity microorganism group at aerobic biologic membrane, be equipped with cathode electrode hole (12) at the top of cathode detection unit (9), cathode carbon cloth
Electrode (14) is drawn by titanium silk (10) and is connect with control unit (17), and cathode detection unit (9) side wall is equipped with solarization air cap (11),
For being inserted into the aerator being connect with air pump (15), cathode detection unit (9) side wall be additionally provided with cathode water inlet (16) and
Cathode water outlet (13) runs cathode detection unit (9) with continuous stream mode.
6. anode and cathode communicating pipe (8) as claimed in claim 3, it is characterised in that anode and cathode communicating pipe (8) one end connection sun
The anode water outlet (7) of pole detection unit (2), the other end connect the cathode water inlet (16) of cathode detection unit (9), communicating pipe
Internal diameter is 2-10mm, pipe range 20-50mm.
7. control unit (17) described in claim 1, it is characterised in that can be fired to continuous flow without film biological-cathode microbiological
Expect that battery I (1) and II applies the voltage of 0-1V, control precision is not less than 1mV, while can measure continuous flow without film biological-cathode
Microbiological fuel cell I (1) and II output electric current, measurement accuracy is not less than 1 μ A, and measurement frequency is not less than 1Hz, when detection,
Control unit (17) according to formula (1) calculating current variation coefficient R, can judge whether the water quality of water sample to be measured is abnormal:
R=| (I1-I2)/I2| × 100% (1)
I1For output electric current of the continuous flow without film biological-cathode microbiological fuel cell I (1) for being continually fed into water sample to be measured, I2For
It is continually fed into output electric current of the continuous flow without film biological-cathode microbiological fuel cell II of standard water sample, I1And I2It is detection
Real-time current per minute in the process determines that water quality is abnormal, otherwise water quality is normal as R > 20.
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| CN109950585A (en) * | 2019-01-31 | 2019-06-28 | 华中科技大学 | A method of promoting microbiological fuel cell electricity production and sensing capabilities |
| CN111948271A (en) * | 2020-07-24 | 2020-11-17 | 北京航空航天大学 | Method for detecting water quality biotoxicity by regulating electrode potential and strengthening microbial electrochemical system |
| CN113358722A (en) * | 2021-04-12 | 2021-09-07 | 北京航空航天大学 | Method for realizing rapid detection of water toxicity based on suspended electrochemical active microorganisms |
| WO2021190152A1 (en) * | 2020-03-27 | 2021-09-30 | 冯中初 | Chlorinator electrode protection method and protection device |
| CN114487068A (en) * | 2021-12-31 | 2022-05-13 | 南开大学 | Method for improving copper ion sensing performance of MFC (micro-fluidic control) by utilizing micro-fluidic technology |
| WO2022237837A1 (en) * | 2021-05-11 | 2022-11-17 | 山东建筑大学 | Method for monitoring water quality by using wetland microbial fuel cell |
| GB2613676A (en) * | 2021-05-11 | 2023-06-14 | Univ Shandong Jianzhu | Method for monitoring water quality by using wetland microbial fuel cell |
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