CN105733980B - The double end bacterium of one plant of aerobic degradation graphene oxide and its application - Google Patents
The double end bacterium of one plant of aerobic degradation graphene oxide and its application Download PDFInfo
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- CN105733980B CN105733980B CN201610054906.0A CN201610054906A CN105733980B CN 105733980 B CN105733980 B CN 105733980B CN 201610054906 A CN201610054906 A CN 201610054906A CN 105733980 B CN105733980 B CN 105733980B
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- C—CHEMISTRY; METALLURGY
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- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- A—HUMAN NECESSITIES
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- A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
- A62D3/00—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances
- A62D3/02—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances by biological methods, i.e. processes using enzymes or microorganisms
Abstract
The double end bacterium of one plant of aerobic degradation graphene oxide and its application, belong to microorganisms technical field.The strain isolation is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on April 9th, 2015 from pedotheque, and deposit number is CGMCC No.10702.The bacterial strain through 16S rRNA gene sequencing, with double end bacterium (Labrys) similitude (99%) with higher, therefore the bacterium classification naming isLabrys Sp.WJW, the GenBank accession number of bacterial strain 16S rRNA gene order are KR778886.The bacterial strain in minimal medium can using graphene oxide as sole carbon source grow, and can redox graphene functional group, destroy its two-dimensional structure, biodegrade carried out to it, show the bacterial strain nano material degrade the potential application value in field.
Description
Technical field
The double end bacterium for capableing of aerobic degradation graphene oxide the present invention relates to one plant and its application belong to microbial technique neck
Domain.
Technical background
Graphite alkenes material is the popular c-based nanomaterial for being found and being studied successively in recent years, because of its mechanics, quantum
It is excellent with electrical properties, paid attention to extensively by physics and material educational circles.Compared with expensive fullerene and carbon nanotube, stone is aoxidized
Black alkene is cheap, and raw material is easy to get, and is expected to the high quality filler as polymer nanocomposites.
The resource reserve of China's graphite mineral products is big, quality, and yield and outlet occupy first place in the world.In recent years, graphite
Deep processing production graphite alkenes material has obtained national more and more attention, " made in China 2025 " major fields technology path
The graphene yield overall goal of (2015 editions) announcements of figure is that " the year two thousand twenty forms 10,000,000,000 industry sizes, whole industry rule in 2025
Mould breaks through hundred billion ".The synthesis of graphene oxide mainly passes through chemical oxidation method, and prepares graphene oxide from graphite and also recognized
For the strategic starting point for being extensive synthesizing graphite alkene.The chemical oxidation method being widely used at present prepares and purifies graphene oxide
It is required to using washing filtering, to generate the largely waste water containing graphene oxide and by-product.Simultaneously with stone
Black alkenes material more and more puts into application in Material Field, the discharge materials such as graphene oxide into environment institute there may be
Environment influence can not be ignored, researches show that excessive graphene oxide enter in vivo can the organs such as lung to mammal generate
Toxicity, experiment in vitro, which also shows it, there is toxicity to lead to cell inactivation microorganism.
For graphene oxide as a kind of biggish two-dimension nano materials of scale, it is difficult to degrade.Existing nanometer material
Material chemical degradation method such as light helps Fenton oxidation method to need to add a large amount of strong protonic acid and oxidant.The biology drop of graphene oxide
Solution research is less at present, and Kotchey et al. reports the case where horseradish peroxidase is in lasting addition hydrogen peroxide in 2011
Lower degradation graphene oxide can gradually degrade in graphene sheet layer structure and generate cavity.But up to now without utilizing micro- life
The relevant report of object cell degradation graphene oxide.In terms of the biodegradable research of other nano materials, Allen et al. discovery
Horseradish peroxidase can degrade single-walled carbon nanotube, while also having been reported that and confirm that manganese peroxidase has the single wall that can degrade
Carbon nanotube.In animal ferment field, Kagan et al. discovery myeloperoxidase can degrade carbon nanotube.What this patent was related to
Double end bacterium (Labrys) it is a kind of gramnegative bacterium, there is the energy degraded and grown using graphene oxide as sole carbon source
Power.
Summary of the invention
The purpose of the present invention is to provide one plant of double end bacterium, and use the strains for degrading graphene oxide, which exists
It is had potential application in biodegradable field of nanometer material technology.
The present invention relates to the double end bacterium of one plant of aerobic degradation graphene oxide (Labrys sp. WJW), which steps on
Charging to volume number is CGMCC No.10702, is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center,
Depositary institution address be Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica, the deposit date is
On April 9th, 2015.Registration number of the 16S rRNA gene order of bacterial strain WJW in GenBank database is KR778886.
From pedotheque, soil is derived near the Dadong District of Shenyang City, Liaoning Province the strain isolation.The bacterial strain is cultivated in LB
It is aerobic under 30 DEG C and 150 r/min in base to cultivate 1 day, the white muddy shape of bacterium solution.The bacterial strain can be in minimal medium
In using graphene oxide as sole carbon source grow, and have degradation graphene oxide ability.LB culture medium composition are as follows: peptone
10 g/L of 10 g/L, yeast powder 5 g/L, NaCl；pH=7.Minimal medium composition are as follows: (NH4)2SO42 g/L, KH2PO4
2 g/L, Na2HPO41.3 g/L, FeCl30.25 mg/L.Solid medium is that 20 g/L fine jades are added in corresponding fluid nutrient medium
Rouge.
The bacterial strain is applied to the degradation of graphene oxide.The single colonie of bacterial strain WJW in solid medium is chosen into containing
In the minimal medium of 50 mg/L and 100 mg/L graphene oxides, shaken cultivation under the conditions of 30 DEG C and 150 r/min
It can grow and graphene oxide of degrading, generate black precipitate.Show bacterium using transmission electron microscope and atomic force microscope observation
Strain WJW can destroy the two-dimensional structure of graphene oxide, generate apparent cavity.Raman chromatography shows bacterial strain to oxidation stone
The reduction of black alkene occurs mainly in initial 5 days.Gas chromatography-mass spectrometry analysis shows that bacterial strain WJW can degrade oxidation
Graphene simultaneously generates a series of intermediate product.
The beneficial effects of the present invention are: the bacterial strain is tamed isolated from pedotheque, soil is derived from ShenYang, Liaoning Province
Near city Dadong District, through 16S rRNA sequence analysis shows the bacterial strain belongs to double end Pseudomonas.The bacterial strain can be in inorganic salts culture
It is grown in base using graphene oxide as sole carbon source and graphene oxide of degrading.The bacterial strain is that the first can be dropped at present with microorganism
The bacterium for solving graphene oxide, provides new microbial resources for the biodegrade of nano material, has and potentially applies valence
Value.
Detailed description of the invention
The phylogenetic tree of Fig. 1 double end bacterium WJW.
Fig. 2 double end bacterium WJW is using graphene oxide as the growth curve of sole carbon source.
The electron microscopic picture of Fig. 3 double end bacterium WJW degradation graphene oxide system.
Fig. 4 double end bacterium WJW acts on 0 day (a), and 5 days (b), the Raman spectrum of graphene oxide when 10 (c) day.
The atomic force microscope characterization of graphene oxide after Fig. 5 double end bacterium WJW effect.
The intermediate product that Fig. 6 double end bacterium WJW degradation graphene oxide generates.
The intermediate product that Fig. 7 double end bacterium WJW degradation graphene oxide generates.
The intermediate product that Fig. 8 double end bacterium WJW degradation graphene oxide generates.
The intermediate product that Fig. 9 double end bacterium WJW degradation graphene oxide generates.
The intermediate product that Figure 10 double end bacterium WJW degradation graphene oxide generates.
The intermediate product that Figure 11 double end bacterium WJW degradation graphene oxide generates.
The intermediate product that Figure 12 double end bacterium WJW degradation graphene oxide generates.
Specific embodiment
Embodiment 1: the acquisition of bacterial strain of the present invention
Double end bacterium of the present invention is isolated from the soil near the Dadong District of Shenyang City, Liaoning Province.Early period is first by soil sample
It is placed in microorganism reactor and tames culture, made after initial 60 days using 50 mg/L glucose and 30 mg/L graphene oxides
For carbon source domestication, then further tamed only with 30 mg/L graphene oxides as carbon source.Domestication takes 2 mL anti-after 120 days
Device mud mixture is answered, using dilution plating procedure, sample is coated on the solid inorganic salt medium containing graphene oxide
(30 mg/L graphene oxides, (NH4)2SO42 g/L, KH2PO42 g/L, Na2HPO41.3 g/L, FeCl3 0.25 mg/
L, 20 g/L of agar) on, it is cultivated 3 days at 30 DEG C.After culture dish grows bacterium colony, a small amount of bacterium colony of picking contains 30 mg/ in 25 mL
In 50 mL conical flasks of L graphene oxide liquid inorganic salt culture medium, the shaken cultivation under 30 DEG C, 150 r/min, to bacterial strain
Increased logarithmic phase is grown to, a small amount of bacterium solution dilution is drawn and carries out plate coating.Aforesaid operations are repeated, until the bacterium purified
Strain, is named as WJW.
The bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on April 9th, 2015
(Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica), number of registering on the books are CGMCC No.
10702。
Embodiment 2: the 16S rRNA Molecular Identification of bacterial strain
The genomic DNA for extracting bacterial strain WJW, by PCR amplification 16S rRNA gene order and is sequenced, examining order is by big
Lian Bao bioengineering Co., Ltd completes.Cognate pair ratio is carried out to sequence using blast program, is obtained and its most similar strain
For double end bacterium, the two 16S rRNA gene order similitude is 100%, therefore, it is determined that bacterial strain WJW is double end bacterium.Fig. 1 is bacterial strain
The phylogenetic tree of WJW gene.Its 16S rRNA sequence has logged in GenBank database, and entering hiding number is KR778886.Bacterial strain
The 16S rRNA gene order of WJW is as follows.
>WJW 16S rRNA gene sequence
ACGCTGGCGGCAGGCTTAACACATGCAAGTCGAACGCATCCTTCGGGGTGAGTGGCAGACGGGTGAGT
AACGCGTGGGGATGTGCCTTGAGGTGGGGAATAACTGTGGGAAACTACAGCTAATACCGCATAAGCCCTTCGGGGG
AAAGATTTATCGCCTTTAGAGCAACCCGCGTCAGATTAGCTAGTTGGTAGGGTAATGGCCTACCAAGGCGACGATC
TGTAGCTGGTCTGAGAGGATGACCAGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGG
GGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGAGTGATGACGGCCTTAGGGTTGTAAAGCTC
TTTTAACAGGGACGATAATGACGGTACCTGTAGAATAAGCCCCGGCAAACTTCGTGCCAGCAGCCGCGGTAATACG
AAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTAGGCGGATTGTTAAGTCGGGGGTGAAATCCT
GAGGCTCAACCTCAGAACTGCCTTCGATACTGGCGATCTTGAGTTCGGAAGAGGTTGGTGGAACAGCTAGTGTAGA
GGTGAAATTCGTAGATATTAGCTAGAACACCAGTGGCGAAGGCGGCCAACTGGTCCGATACTGACGCTGAGGTGCG
AAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAATGCCAGCCGTCGGGGAGC
TTGCTCTTCGGTGGCGCAGCTAACGCTTTAAGCATTCCGCCTGGGGAGTACGGTCGCAAGATTAAAACTCAAAGGA
ATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGCAGAACCTTACCAGCCCTTGA
CATCCCGGTCGCGGATCACAGAGATGAGATCCTTCAGTTCGGCTGGACCGGAGACAGGTGCTGCATGGCTGTCGTC
AGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCGCCCCTAGTTGCCAGCATTGAGTTGG
GCACTCTAGGGGGACTGCCGGTGATAAGCCGCGAGGAAGGTGGGGATGACGTCAAGTCCTCATGGCCCTTACGGGC
TGGGCTACACACGTGCTACAATGGCGGTGACAGTGGGAAGCGAAGGGGTGACCCCTAGCAAATCTCCAAAAGCCGT
CTCAGTTCAGATTGCACTCTGCAACTCGAGTGCATGAAGGTGGAATCGCTAGTAATCGCAGATCAGCATGCTGCGG
TGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTTGGTTTTACCCGAAGGCGCTGCGCCAA
CCGCAAGGAGGCAGGCGACCACGGTAGGGTCAGCGACTGGG。
Embodiment 3: bacterial strain WJW is using graphene oxide as the growth curve of sole carbon source
Using the bacterium solution in embodiment 1, using 121 DEG C, the minimal medium ((NH of 20 min high pressure moist heat sterilizations4)2SO42 g/L, KH2PO42 g/L, Na2HPO41.3 g/L, FeCl30.25 mg/L), 50 mg/L and 100 mg/L are added
Graphene oxide, the shaken cultivation under the conditions of 30 DEG C, 150 r/min, inoculum concentration 20%.Thalli growth amount uses ultraviolet spectrometry
Photometry detects bacterium solution absorbance under 660 nm, the growing state of bacterial strain WJW such as Fig. 2.The result shows that bacterial strain WJW can be with oxidation
Graphene is sole carbon source growth, and bacterial strain enters stable growth period, the growth of bacterial strain WJW degradation graphene oxide after culture 24 days
Amount increases comparatively fast in initial 3 days, and increment increases more steady during 3-24 days.With the graphene oxide registered at present
Biodegrading process is compared, and bacterial strain WJW can be raw by sole carbon source of graphene oxide in the presence of only a small amount of inorganic salts
It grows and graphene oxide of degrading, while experiment shows that it is cultivated 180 days under the conditions of having existing for graphene oxide and still maintains
Existence is not required to add other drugs, and degradation mode is low in cost, environmentally protective.
Embodiment 4: bacterial strain WJW and graphene oxide act on transmission electron microscope
Utilizing graphene oxide according to the method for embodiment 3 is that sole carbon source culture bacterial strain WJW is negated after reaction 20 days
Liquid is answered to carry out transmission electron microscope characterization, as shown in Figure 3.Bacterial strain WJW be bacillus, thallus can be made with oxidation graphene secondly
Dimension structure is destroyed, and apparent cavity is generated, and shows that bacterial strain WJW can carry out biodegrade to graphene oxide.
Embodiment 5: bacterial strain WJW acts on the Raman Characterization of graphene oxide after different time
Utilizing graphene oxide according to the method for embodiment 3 is sole carbon source culture bacterial strain WJW, takes the 0th, 5,10,15,30
It reaction solution carries out Raman Characterization.1360 cm of Raman spectrogram-1With 1600 cm-1The peak of left and right is respectively graphene
The peak characteristic peak D, G of substance, ID/IGVariation the case where showing graphene substance structural failure.At the 0th, 5,10 day
ID/IGRaman spectrum is then without significant change when value is respectively 0.92,1.035,1.061, the 10th to 30 day.Fig. 4 is the 0th, 5,10
Raman spectrum (normalization mapping).Raman spectrum shows that bacterial strain has degradation to graphene oxide, in graphene two dimension
Defect is generated in structure results in ID/IGThe rising of value, and initial 10 days are very fast, subsequent variation is not primarily due to this experiment very much
It connects that bacterium amount is less and thalli growth is limited compared with slow (sole carbon source graphene oxide degrade difficulty larger) and Raman experiments detection site
System.
Embodiment 6: the atomic force microscope characterization of graphene oxide after double end bacterium WJW effect
Utilizing graphene oxide according to the method for embodiment 3 was sole carbon source culture bacterial strain WJW, by the 20th day reaction solution
Atomic force microscope characterization is carried out, as shown in Figure 5, it was demonstrated that graphene sheet layer cavity is produced around thallus, thickness is about one
Layer graphene oxide thickness.The graphene oxide two-dimensional structure being attached to around thallus the result shows that bacterial strain WJW can degrade.
Embodiment 7: the intermediate product analysis that double end bacterium WJW degradation graphene oxide generates
Bacterium solution in embodiment 3 is transferred into LB culture medium (10 g/L of peptone, yeast powder 5 g/L, NaCl 10
g/L；PH=7) in large-scale culture, the bacterium solution of acquirement uses 5000 × g, 5 min to be collected by centrifugation, slow using phosphate after collection
It rushes solution (50 mmol/L, pH=7.0) and cleaning 3 times is resuspended, centrifugal condition is 5000 × g, 5 min.Use inorganic salts culture
Thallus is resuspended base, modulates OD660It is 2.0,200 mg/L graphene oxides is added, system is 100 mL, in 30oC, 150
Shaken cultivation under the conditions of r/min.2 mL of sample when taking the 18th day, uses isometric CHCl3Extraction, sample utilizes gas after extraction
Phase chromatograph-mass spectrometer coupling analyzes product therein, and Fig. 6-12 is partial intermediate.Product is mostly the substance containing phenyl ring, oxygen
The functional group of graphite alkene does not have phenyl ring, therefore the two-dimensional structure that these intermediate products are bacterial strain destruction graphene oxides is broken
, it was confirmed that bacterial strain can be such that the two-dimensional structure ontology of graphene oxide destroys, to carry out biodegrade to it.
<110>Dalian University of Technology
The double end bacterium of<120>one plants of aerobic degradation graphene oxides and its application
<210> 1
<211> 1401
<212> DNA
<213>double end bacterium (Labrys sp.)
<400> 1
ACGCTGGCGGCAGGCTTAACACATGCAAGTCGAACGCATCCTTCGGGGTG50
AGTGGCAGACGGGTGAGTAACGCGTGGGGATGTGCCTTGAGGTGGGGAAT100
AACTGTGGGAAACTACAGCTAATACCGCATAAGCCCTTCGGGGGAAAGAT150
TTATCGCCTTTAGAGCAACCCGCGTCAGATTAGCTAGTTGGTAGGGTAAT200
GGCCTACCAAGGCGACGATCTGTAGCTGGTCTGAGAGGATGACCAGCCAC250
ACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAA300
TATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGAGTGATGA350
CGGCCTTAGGGTTGTAAAGCTCTTTTAACAGGGACGATAATGACGGTACC400
TGTAGAATAAGCCCCGGCAAACTTCGTGCCAGCAGCCGCGGTAATACGAA450
GGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTAGGCGGA500
TTGTTAAGTCGGGGGTGAAATCCTGAGGCTCAACCTCAGAACTGCCTTCG550
ATACTGGCGATCTTGAGTTCGGAAGAGGTTGGTGGAACAGCTAGTGTAGA600
GGTGAAATTCGTAGATATTAGCTAGAACACCAGTGGCGAAGGCGGCCAAC650
TGGTCCGATACTGACGCTGAGGTGCGAAAGCGTGGGGAGCAAACAGGATT700
AGATACCCTGGTAGTCCACGCCGTAAACGATGAATGCCAGCCGTCGGGGA750
GCTTGCTCTTCGGTGGCGCAGCTAACGCTTTAAGCATTCCGCCTGGGGAG800
TACGGTCGCAAGATTAAAACTCAAAGGAATTGACGGGGGCCCGCACAAGC850
GGTGGAGCATGTGGTTTAATTCGAAGCAACGCGCAGAACCTTACCAGCCC900
TTGACATCCCGGTCGCGGATCACAGAGATGAGATCCTTCAGTTCGGCTGG950
ACCGGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTT1000
GGGTTAAGTCCCGCAACGAGCGCAACCCTCGCCCCTAGTTGCCAGCATTG1050
AGTTGGGCACTCTAGGGGGACTGCCGGTGATAAGCCGCGAGGAAGGTGGG1100
GATGACGTCAAGTCCTCATGGCCCTTACGGGCTGGGCTACACACGTGCTA1150
CAATGGCGGTGACAGTGGGAAGCGAAGGGGTGACCCCTAGCAAATCTCCA1200
AAAGCCGTCTCAGTTCAGATTGCACTCTGCAACTCGAGTGCATGAAGGTG1250
GAATCGCTAGTAATCGCAGATCAGCATGCTGCGGTGAATACGTTCCCGGG1300
CCTTGTACACACCGCCCGTCACACCATGGGAGTTGGTTTTACCCGAAGGC1350
GCTGCGCCAACCGCAAGGAGGCAGGCGACCACGGTAGGGTCAGCGACTGG1400
G
Claims (1)
1. one plant of aerobic degradation graphene oxide double end bacterium (Labrys sp. ) WJW application, the registration of the double end bacterium enters
Volume number is CGMCC No.10702, is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preservation
Unit address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, and Institute of Microorganism, Academia Sinica, the deposit date is 2015
On April 9, in；It is characterized by: the double end bacterium is applied to the degradation of graphene oxide.
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Citations (5)
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|---|---|---|---|---|
| CN103395775A (en) * | 2013-07-29 | 2013-11-20 | 南京理工大学 | Grapheme oxide reduced by microbial fuel cell anode microorganisms and preparation method thereof |
| CN103408002A (en) * | 2013-07-29 | 2013-11-27 | 南京理工大学 | Microbial reduction of graphene oxide and preparation method for graphene |
| CN103756936A (en) * | 2014-01-09 | 2014-04-30 | 杭州宝晶生物股份有限公司 | Labrys and method for producing L(+)-tartaric acid or salt thereof by utilizing same |
| CN103931037A (en) * | 2011-11-16 | 2014-07-16 | 国立大学法人丰桥技术科学大学 | Microbial power generation device, electrode for microbial power generation device, and method for producing same |
| CN104176732A (en) * | 2014-08-20 | 2014-12-03 | 南京工业大学 | Method for preparing graphene through biological catalysis |
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- 2016-01-27 CN CN201610054906.0A patent/CN105733980B/en not_active Expired - Fee Related
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| CN103931037A (en) * | 2011-11-16 | 2014-07-16 | 国立大学法人丰桥技术科学大学 | Microbial power generation device, electrode for microbial power generation device, and method for producing same |
| CN103395775A (en) * | 2013-07-29 | 2013-11-20 | 南京理工大学 | Grapheme oxide reduced by microbial fuel cell anode microorganisms and preparation method thereof |
| CN103408002A (en) * | 2013-07-29 | 2013-11-27 | 南京理工大学 | Microbial reduction of graphene oxide and preparation method for graphene |
| CN103756936A (en) * | 2014-01-09 | 2014-04-30 | 杭州宝晶生物股份有限公司 | Labrys and method for producing L(+)-tartaric acid or salt thereof by utilizing same |
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