CN103100114B - Preparation method of medical metal surface slow-released growth factor coating - Google Patents
Preparation method of medical metal surface slow-released growth factor coating Download PDFInfo
- Publication number
- CN103100114B CN103100114B CN201310024516.5A CN201310024516A CN103100114B CN 103100114 B CN103100114 B CN 103100114B CN 201310024516 A CN201310024516 A CN 201310024516A CN 103100114 B CN103100114 B CN 103100114B
- Authority
- CN
- China
- Prior art keywords
- somatomedin
- solution
- graphene oxide
- medical metal
- nano
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Materials For Medical Uses (AREA)
- Medicinal Preparation (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses a preparation method of a medical metal surface slow-released growth factor coating. The method comprises the steps of firstly, using graphene oxide as the growth factor carrier, wrapping growth factors in composite nano granules formed by polycations and polyanions, reacting the nano granules containing the growth factors with the carboxylated graphene oxide so as to form amido bonds, thereby fixing the nano granules on the graphene oxide; and enabling the graphene oxide carrying the growth factors to form the coating on a biologic medical metal surface which is subjected to dopamine treatment by using an extraction method. By utilizing the preparation method, higher growth factor carrying quantity is realized, and meanwhile, the activity of the factors is protected, and the slow-releasing of the factors is realized.
Description
Technical field
The present invention relates to the preparation method of the slow release somatomedin coating on a kind of medical metal surface.
Background technology
Medical metal is because its good biocompatibility and good mechanical property are widely used in the field such as surgical plastic, bone implant.But medical metal is all biologically inert conventionally, and a little less than its surperficial bone regeneration capability, new bone growth is slow.After this causes medical metal to implant, and can not form fast firmly bonding between surrounding bone tissue.In order to improve the integration ability between medical metal implant and osseous tissue, often need it to carry out surface modification.Wherein a kind of method of surface modification is the regeneration that is written into cell growth factor and promotes osseous tissue at medical metal implant surfaces.Common cell growth factor, as bone morphogenetic protein (BMP-2), angiogenesis factor (VEGF), conversion growth factor (TGF-β 2) etc. play a part crucial in bone repair process.The method of loading at present somatomedin has directly somatomedin is adsorbed on to material surface, but this method cannot realize the slow releasing function of somatomedin, and somatomedin is exposed to and in physiological environment, is easy to be subject to the impact of various factors and inactivation; Also have biomedical metallic material after treatment is combined in the mode of covalent bond with somatomedin, though this mode can realize the immobilized of somatomedin, but covalent bonds mode tends to cause somatomedin inactivation.
Summary of the invention
The object of this invention is to provide the preparation method of the slow release somatomedin coating on a kind of medical metal surface, the somatomedin load capacity of the standby slow release somatomedin coating of this legal system is high, somatomedin can slowly discharge with body in new bone growth speed adaption, and the activity of somatomedin is strong.
The present invention realizes the technical scheme that its goal of the invention adopts, the preparation method of a kind of medical metal surface slow release somatomedin coating, and its concrete steps are as follows:
The parcel of A, somatomedin:
The somatomedin that osteogenesis is had to facilitation is dissolved in the polyanion solution that concentration is 0.5-2.5mg/ml, obtains the polyanion solution that contains somatomedin, the concentration of somatomedin is 0.5-10 μ g/ml.
By described containing the polyanion solution of somatomedin and the said polycation solution of the band amino that concentration is 0.5-2.5mg/ml, by volume 1: the ratio mixing of 2-5, and stirring, centrifugal, lyophilization, obtain being loaded with the nano-particle of somatomedin;
B, graphene oxide carboxylated:
The ClCH that is 20mg/ml by concentration
2cOONa solution and concentration are that the NaOH solution of 20mg/ml is mixed with mixed solution for 1: 1 by volume, then the graphene oxide of 80mg are added in the mixed solution of 4-6ml and react 1.5-2.5h, make the oxy radical on graphene oxide carboxylated; Then by solution dialysis lyophilizing, obtain carboxylated graphene oxide powder.
C, nano-particle immobilized:
B is walked to the nano-particle that carries somatomedin that the carboxylated graphene oxide powder that obtains and A step make and carry out EDC/NHS catalytic reaction, make the carboxyl on carboxylated graphene oxide surface and the amino of nano grain surface form the nano-particle of amido link, year somatomedin immobilized on graphene oxide; Then by reactant liquor dialysis, lyophilization, then dry parts is dissolved in the buffer solution of pH value 6.0, forms graphene oxide/nanoparticles solution;
D, by the medical metal material that is grafted with dopamine coating lixiviate in the graphene oxide/nanoparticles solution of C step, make load have the graphite oxide ene coatings of somatomedin to be combined in medical metal material surface by Electrostatic Absorption.
Compared with prior art, the invention has the beneficial effects as follows:
One, with directly load somatomedin on substrate material surface compared with, the present invention is first wrapped in somatomedin in nano-particle and is combined with graphene oxide, load in the process of material surface at somatomedin, the electrostatic interaction only depending between somatomedin and polyelectrolyte ion is combined, do not relate to chemical reaction, the activity of somatomedin can not be damaged, and can keep well the activity of somatomedin.
Two, graphene oxide is the Graphene derivant of surface with multiple oxy radical.It has monoatomic layer network structure structure, and specific surface area is very large, the nano-particle that is loaded with somatomedin can be wrapped up and load capacity large, can realize the high-efficient carrier of medicine, and can regulate and control preferably the slow release of somatomedin.
Above-mentioned medical metal is titanium, titanium alloy or rustless steel.
Above-mentioned somatomedin is BMP-2, VEGF or TGF-β 2.These somatomedin have obvious facilitation to osteogenesis.
Above-mentioned polycation is chitosan or polylysine, and polyanion is heparin, chondroitin sulfate, sodium alginate or hyaluronic acid.
Below in conjunction with the specific embodiment, the invention will be further described.
The specific embodiment
Embodiment mono-
A preparation method for medical metal surface slow release somatomedin coating, its concrete steps are as follows:
The parcel of A, somatomedin:
The growth factor B MP-2 that osteogenesis is had to facilitation is dissolved in the chondroitin sulfate cellulose solution that concentration is 0.5mg/ml, obtain the chondroitin sulfate cellulose solution that contains BMP-2, and the concentration of BMP-2 in chondroitin sulfate cellulose solution is 0.5 μ g/ml;
By described described containing the chondroitin sulfate cellulose solution of growth factor B MP-2 and the polylysine solution of 0.5mg/ml, by the volume ratio mixing of 1: 2, and stirring, centrifugal, lyophilization, obtain being loaded with the nano-particle of BMP-2;
B, graphene oxide carboxylated:
The ClCH that is 20mg/ml by concentration
2cOONa solution and concentration are that the NaOH80mg solution of 20mg/ml is to be mixed with mixed solution at 1: 1 by volume, then 80mg graphene oxide is added in 6ml mixed solution and reacts 2.5h, make the oxy radical on graphene oxide carboxylated; Then by solution dialysis lyophilizing, obtain carboxylated graphene oxide powder;
C, nano-particle immobilized:
B is walked to the nano-particle that carries somatomedin that the carboxylated graphene oxide powder that obtains and A step make and carry out EDC/NHS catalytic reaction, make the carboxyl on carboxylated graphene oxide surface and the amino of nano grain surface form the nano-particle of amido link, year somatomedin immobilized on graphene oxide; Then by reactant liquor dialysis, lyophilization, then dry parts is dissolved in the buffer solution of pH value 6.0, forms graphene oxide/nanoparticles solution;
Wherein, EDC/NHS catalytic reaction is prior art, and its concrete operations can be:
C1, the carboxylated graphene oxide of B step preparation is dissolved in to MES (MES) (0.1M) in solution, obtaining concentration is the MES solution of the carboxylated graphene oxide of 1mg/ml; Be the EDC (1-ethyl-3-(3-dimethyl aminopropyl)-carbodiimides 1-ethyl-3-(3-dimethyl aminopropyl)-carbodiimides) of 2mM and the NHS (N-hydroxy-succinamide) that 0.6mg concentration is 5mM to adding 0.4mg concentration in the MES solution of the carboxylated graphene oxide of 1ml, pH is adjusted to 5～6; Under room temperature, react 15min; In solution, add the 2 mercapto ethanol (2-mercapto ethanol) that 1.4 μ l concentration are 20mM to neutralize unnecessary EDC again;
C2, the nano-particle that is loaded with growth factor B MP-2 prepared by A step, evenly be dissolved in phosphate buffered solution (PBS), obtaining concentrations of nanoparticles is the phosphate buffered solution solution of 1mg/ml, getting this solution of 1ml adds C1 to walk in the solution obtaining, regulate pH to 7.2, under room temperature, react 2h.Finally adding concentration is that the azanol (10mM) of 10mM neutralizes unreacted NHS.
D, will be grafted with titanium alloy lixiviate in the graphene oxide/nanoparticles solution of C step of dopamine coating, electronegative load has the graphene oxide of nano-particle and the dopamine coating of positively charged by Electrostatic Absorption, thereby makes load have the graphene oxide of nano-particle to be combined in titanium alloy surface.
Embodiment bis-
A preparation method for medical metal surface slow release somatomedin coating, its concrete steps are as follows:
The parcel of A, somatomedin:
The growth factor VEGF that osteogenesis is had to facilitation is dissolved in the heparin solution that concentration is 1.5mg/ml, obtain the heparin solution that contains VEGF, and in heparin solution, the concentration of VEGF is 5 μ g/ml;
Containing the heparin solution of growth factor VEGF and the chitosan solution of 2.5mg/ml, by the volume ratio mixing of 1: 4, and stirring, centrifugal, lyophilization, obtain being loaded with the nano-particle of BMP-2 by described;
B, graphene oxide carboxylated:
The ClCH that is 20mg/ml by concentration
2cOONa solution and concentration are that the NaOH80mg solution of 20mg/ml is to be mixed with mixed solution at 1: 1 by volume, then 80mg graphene oxide is added in 5ml mixed solution and reacts 2h, make the oxy radical on graphene oxide carboxylated; Then by solution dialysis lyophilizing, obtain carboxylated graphene oxide powder;
C, nano-particle immobilized:
B is walked to the nano-particle that carries somatomedin that the carboxylated graphene oxide powder that obtains and A step make and carry out EDC/NHS catalytic reaction, make the carboxyl on carboxylated graphene oxide surface and the amino of nano grain surface form the nano-particle of amido link, year somatomedin immobilized on graphene oxide; Then by reactant liquor dialysis, lyophilization, then dry parts is dissolved in the buffer solution of pH value 6.0, forms graphene oxide/nanoparticles solution;
Wherein, EDC/NHS catalytic reaction is prior art, and its concrete operations can be:
C1, the carboxylated graphene oxide of B step preparation is dissolved in to MES (MES) (0.1M) in solution, obtaining concentration is the MES solution of the carboxylated graphene oxide of 1mg/ml; Be the EDC (1-ethyl-3-(3-dimethyl aminopropyl)-carbodiimides 1-ethyl-3-(3-dimethyl aminopropyl)-carbodiimides) of 2mM and the NHS (N-hydroxy-succinamide) that 0.6mg concentration is 5mM to adding 0.4mg concentration in the MES solution of the carboxylated graphene oxide of 1ml, pH is adjusted to 5; Under room temperature, react 15min; In solution, add the 2 mercapto ethanol (2-mercapto ethanol) that 1.4 μ l concentration are 20mM to neutralize unnecessary EDC again;
C2, the nano-particle that is loaded with growth factor VEGF prepared by A step, evenly be dissolved in phosphate buffered solution (PBS), obtaining concentrations of nanoparticles is the phosphate buffered solution solution of 1mg/ml, getting this solution of 1ml adds C1 to walk in the solution obtaining, regulate pH to 7.2, under room temperature, react 2h.Finally adding concentration is that the azanol (10mM) of 10mM neutralizes unreacted NHS.
D, will be grafted with titanium lixiviate in the graphene oxide/nanoparticles solution of C step of dopamine coating, electronegative load has the graphene oxide of nano-particle and the dopamine coating of positively charged by Electrostatic Absorption, thereby makes load have the graphene oxide of nano-particle to be combined in titanium surface
Embodiment tri-
A preparation method for medical metal surface slow release somatomedin coating, its concrete steps are as follows:
The parcel of A, somatomedin:
The somatomedin TGF-β 2 that osteogenesis is had to facilitation is dissolved in the sodium alginate solution that concentration is 2.5mg/ml, obtain the sodium alginate soln that contains TGF-β 2, and the concentration of TGF-β 2 is 10 μ g/ml;
By the described sodium alginate soln containing somatomedin TGF-β 2 and the chitosan solution of 1.0mg/ml, by the volume ratio mixing of 1: 5, and stirring, centrifugal, lyophilization, obtain being loaded with the nano-particle of BMP-2;
B, graphene oxide carboxylated:
The ClCH that is 20mg/ml by concentration
2cOONa solution and concentration are that the NaOH80mg solution of 20mg/ml is to be mixed with mixed solution at 1: 1 by volume, then 80mg graphene oxide is added in 4ml mixed solution and reacts 1.5h, make the oxy radical on graphene oxide carboxylated; After reaction, by solution dialysis lyophilizing, obtain carboxylated graphene oxide powder;
C, nano-particle immobilized:
B is walked to the nano-particle that carries somatomedin that the carboxylated graphene oxide powder that obtains and A step make and carry out EDC/NHS catalytic reaction, make the carboxyl on carboxylated graphene oxide surface and the amino of nano grain surface form the nano-particle of amido link, year somatomedin immobilized on graphene oxide; Then by reactant liquor dialysis, lyophilization, then dry parts is dissolved in the buffer solution of pH value 6.0, forms graphene oxide/nanoparticles solution;
Wherein, EDC/NHS catalytic reaction is prior art, and its concrete operations can be:
C1, the carboxylated graphene oxide of B step preparation is dissolved in to MES (MES) (0.1M) in solution, obtaining concentration is the MES solution of the carboxylated graphene oxide of 1mg/ml; Be the EDC (1-ethyl-3-(3-dimethyl aminopropyl)-carbodiimides 1-ethyl-3-(3-dimethyl aminopropyl)-carbodiimides) of 2mM and the NHS (N-hydroxy-succinamide) that 0.6mg concentration is 5mM to adding 0.4mg concentration in the MES solution of the carboxylated graphene oxide of 1ml, pH is adjusted to 6; Under room temperature, react 15min; In solution, add the 2 mercapto ethanol (2-mercapto ethanol) that 1.4 μ l concentration are 20mM to neutralize unnecessary EDC again;
C2, the nano-particle that is loaded with somatomedin TGF-β 2 prepared by A step, evenly be dissolved in phosphate buffered solution (PBS), obtaining concentrations of nanoparticles is the phosphate buffered solution solution of 1mg/ml, getting this solution of 1ml adds C1 to walk in the solution obtaining, regulate pH to 7.5, under room temperature, react 2h.Finally adding concentration is that the azanol (10mM) of 10mM neutralizes unreacted NHS.
D, will be grafted with rustless steel lixiviate in the graphene oxide/nanoparticles solution of C step of dopamine coating, electronegative load has the graphene oxide of nano-particle and the dopamine coating of positively charged by Electrostatic Absorption, thereby makes load have the graphene oxide of nano-particle to be combined in stainless steel surfaces.
Embodiment tetra-
The operation of this example and embodiment mono-are basic identical, different only: change by chondroitin sulfate the polyanion of preparing nano-particle in example one into hyaluronic acid.
The medical metal material that is grafted with dopamine coating using in the present invention, can adopt prior art to make, as adopted following methods to make:
The dopamine of 2mg/ml is dissolved in Tris-hydrochloric acid (tris-HCl) solution of 10mM and regulates pH to 8.5, again the medical metal material after pickling, alkali cleaning is dipped into 12h in solution, then rinse medical metal material three times, just dopamine coating on medical metal material (titanium, titanium alloy, rustless steel) surface grafting.
Claims (3)
1. a preparation method for medical metal surface slow release somatomedin coating, its concrete steps are as follows:
The parcel of A, somatomedin:
The somatomedin that osteogenesis is had to facilitation is dissolved in the polyanion solution that concentration is 0.5-2.5mg/ml, obtains the polyanion solution that contains somatomedin, the concentration of somatomedin is 0.5-10 μ g/ml;
By described, containing the polyanion solution of somatomedin and the said polycation solution of the band amino that concentration is 0.5-2.5mg/ml, the ratio of 1:2-5 mixing by volume, and stirring, centrifugal, lyophilization, obtain being loaded with the nano-particle of somatomedin;
Described polycation is chitosan or polylysine, and described polyanion is chondroitin sulfate, heparin, sodium alginate or hyaluronic acid;
B, graphene oxide carboxylated:
The ClCH that is 20mg/ml by concentration
2cOONa solution and concentration be 20mg/ml NaOH solution by volume 1:1 be mixed with mixed solution, then the graphene oxide of 80mg is added in the mixed solution of 4-6ml and reacts 1.5-2.5h, make the oxy radical on graphene oxide carboxylated; Then by solution dialysis lyophilizing, obtain carboxylated graphene oxide powder;
C, nano-particle immobilized:
B is walked to the nano-particle that carries somatomedin that the carboxylated graphene oxide powder that obtains and A step make and carry out EDC/NHS catalytic reaction, make the carboxyl on carboxylated graphene oxide surface and the amino of nano grain surface form the nano-particle of amido link, year somatomedin immobilized on graphene oxide; Then by reactant liquor dialysis, lyophilization, then xeraphium is dissolved in the buffer solution of pH value 6.0, forms graphene oxide/nanoparticles solution;
D, by the medical metal material that is grafted with dopamine coating lixiviate in the graphene oxide/nanoparticles solution of C step, make load have the graphite oxide ene coatings of somatomedin to be combined in medical metal material surface by Electrostatic Absorption.
2. the preparation method of a kind of medical metal according to claim 1 surface slow release somatomedin coating, is characterized in that: described medical metal is titanium, titanium alloy or rustless steel.
3. the preparation method of a kind of medical metal according to claim 1 surface slow release somatomedin coating, is characterized in that: described somatomedin is BMP-2, VEGF or TGF-β 2.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310024516.5A CN103100114B (en) | 2013-01-23 | 2013-01-23 | Preparation method of medical metal surface slow-released growth factor coating |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310024516.5A CN103100114B (en) | 2013-01-23 | 2013-01-23 | Preparation method of medical metal surface slow-released growth factor coating |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103100114A CN103100114A (en) | 2013-05-15 |
| CN103100114B true CN103100114B (en) | 2014-05-21 |
Family
ID=48308523
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310024516.5A Expired - Fee Related CN103100114B (en) | 2013-01-23 | 2013-01-23 | Preparation method of medical metal surface slow-released growth factor coating |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103100114B (en) |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103623984A (en) * | 2013-11-27 | 2014-03-12 | 天津大学 | Method for functional modification of growth factor-nanogel of surface of titanium alloy |
| CN104141124A (en) * | 2014-06-17 | 2014-11-12 | 天津大学 | Method for improving biological activity of pure titanium surface by using dopamine to be bonded with graphene oxide |
| CN104531669B (en) * | 2014-12-30 | 2018-09-11 | 苏州英芮诚生化科技有限公司 | A kind of magnetic graphene nanocomposite, the preparation method and its usage of cladding hydrophily poly-dopamine |
| CN105063420B (en) * | 2015-08-06 | 2017-03-08 | 惠安立科贸易有限公司 | A kind of preparation method of the medical titanium alloy bar of strong antibacterial |
| CN105063424B (en) * | 2015-08-06 | 2017-03-22 | 惠安立科贸易有限公司 | Preparation method of medical titanium alloy bar |
| CN105063423B (en) * | 2015-08-06 | 2017-03-08 | 惠安百创贸易有限公司 | A kind of medical graphite alkene strengthens titanium alloy rod bar |
| CN105063421B (en) * | 2015-08-06 | 2017-03-08 | 惠安品科电子科技有限公司 | A kind of medical graphite alkene of strong antibacterial strengthens the preparation method of titanium alloy rod bar |
| CN105268026B (en) * | 2015-10-21 | 2018-07-17 | 武汉理工大学 | A kind of preparation method of chitosan/oxidized graphene/nanometer hydroxyapatite three-dimensional stephanoporate compound stent material |
| CN105343890B (en) * | 2015-11-18 | 2018-02-13 | 山东大学 | A kind of heparin or the graphene oxide of its salt modification and preparation method and application |
| CN107313092B (en) * | 2016-04-27 | 2018-12-25 | 西南交通大学 | A method of on medical metal surface, preparation has Electro-stimulate response composite microcapsule |
| CN106693078A (en) * | 2016-11-24 | 2017-05-24 | 西南交通大学 | Preparation method of drug-loaded layer-by-layer self-assembly coating |
| CN106963982A (en) * | 2017-04-24 | 2017-07-21 | 广东医科大学附属医院 | A kind of repair materials adhered to anti-bacteria wet keeping appropriate tissue and its preparation method and application |
| CN107137788B (en) * | 2017-04-28 | 2019-05-24 | 淮阴工学院 | A method of chitosan/test tube of hepari graphene oxide composite multilayer membrane is prepared in medical magnesium alloy surface |
| CN107158481B (en) * | 2017-05-22 | 2019-12-24 | 淮阴工学院 | Preparation of heparin and Cu on surface of biological material2+Method for coating mesoporous silicon nano particles |
| CN108159496B (en) * | 2018-01-22 | 2020-11-03 | 福州大学 | Bionic oriented cartilage scaffold released by double-factor program and preparation method thereof |
| CN108939085A (en) * | 2018-08-13 | 2018-12-07 | 常州市中医医院 | Bone targeting medicine-carried system and preparation method thereof |
| CN110833631B (en) * | 2018-08-17 | 2021-11-16 | 成都迈德克科技有限公司 | Preparation method of multifunctional intravascular stent |
| CN110433342A (en) * | 2019-08-20 | 2019-11-12 | 曲彦隆 | A kind of preparation method of compound VEGF and rhBMP-2 biphase coating articular prosthesis |
| CN114601962A (en) * | 2020-12-09 | 2022-06-10 | 上海交通大学 | Polydopamine-graphene composite coating on surface of biomedical magnesium alloy and preparation thereof |
| CN112773943A (en) * | 2021-01-11 | 2021-05-11 | 西南医科大学附属中医医院 | Graphene oxide composite platelet-rich plasma sustained-release growth factor hydrogel stent |
| CN113842501B (en) * | 2021-10-21 | 2022-07-01 | 华中科技大学同济医学院附属协和医院 | Surface modification material for internal implant and preparation method and application thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1394901A (en) * | 2002-07-10 | 2003-02-05 | 浙江大学 | Biological material using electrostatic attraction layer-layer self-assembled modified polyester material as surface with cell compatibility |
| CN1791437A (en) * | 2003-05-16 | 2006-06-21 | 布卢薄膜有限责任公司 | Medical implants comprising biocompatible coatings |
| CN101721389A (en) * | 2009-11-30 | 2010-06-09 | 浙江大学 | Method for preparing in-situ therapeutic substance-loaded microcapsules |
| CN102000658A (en) * | 2010-12-15 | 2011-04-06 | 西南交通大学 | Polydopamine-based biofunction modification method |
| CN102433032A (en) * | 2011-09-08 | 2012-05-02 | 南京师范大学 | Controllable method for synthesizing carboxylic graphene oxide and prepared nano material |
| CN102677032A (en) * | 2012-05-24 | 2012-09-19 | 西南交通大学 | Method for immobilizing VEGF-carried heparin/polylysine nanoparticles on Ti surface |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20100117570A (en) * | 2008-01-03 | 2010-11-03 | 내셔널 유니버시티 오브 싱가포르 | Functionalised graphene oxide |
-
2013
- 2013-01-23 CN CN201310024516.5A patent/CN103100114B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1394901A (en) * | 2002-07-10 | 2003-02-05 | 浙江大学 | Biological material using electrostatic attraction layer-layer self-assembled modified polyester material as surface with cell compatibility |
| CN1791437A (en) * | 2003-05-16 | 2006-06-21 | 布卢薄膜有限责任公司 | Medical implants comprising biocompatible coatings |
| CN101721389A (en) * | 2009-11-30 | 2010-06-09 | 浙江大学 | Method for preparing in-situ therapeutic substance-loaded microcapsules |
| CN102000658A (en) * | 2010-12-15 | 2011-04-06 | 西南交通大学 | Polydopamine-based biofunction modification method |
| CN102433032A (en) * | 2011-09-08 | 2012-05-02 | 南京师范大学 | Controllable method for synthesizing carboxylic graphene oxide and prepared nano material |
| CN102677032A (en) * | 2012-05-24 | 2012-09-19 | 西南交通大学 | Method for immobilizing VEGF-carried heparin/polylysine nanoparticles on Ti surface |
Non-Patent Citations (12)
| Title |
|---|
| Chye Khoon Poh et al..The effect of VEGF functionalization of titanium on endothelial cells in vitro.《Biomaterials》.2009,第31卷(第7期),第1578-1585页. |
| Electrochemical Genosensor Based on Colloidal Gold Nanoparticles for the Detection of Factor V Leiden Mutation Using Disposable Pencil Graphite Electrodes;Mehmet Ozsoz et al.;《Analytical Chemistry》;20030403;第75卷(第9期);第2181-2187页 * |
| Grapheme based materials: past, present and future;Virendra Singh et al.;《Progress in Materials Science》;20110403;第56卷(第8期);第1178-1271页 * |
| Mehmet Ozsoz et al..Electrochemical Genosensor Based on Colloidal Gold Nanoparticles for the Detection of Factor V Leiden Mutation Using Disposable Pencil Graphite Electrodes.《Analytical Chemistry》.2003,第75卷(第9期),第2181-2187页. |
| Min Huang et al..Polyelectrolyte Complexes Stabilize and Controllably Release Vascular Endothelial Growth Factor.《Biomacromolecules》.2007,第8卷(第5期),第1607-1614页. |
| Origin of Enhanced Stem Cell Growth and Differentiation on Graphene and Graphene Oxide;Wong Cheng Lee et al.;《ACS Nano》;20110727;第5卷(第9期);第7334-7341页 * |
| Polyelectrolyte Complexes Stabilize and Controllably Release Vascular Endothelial Growth Factor;Min Huang et al.;《Biomacromolecules》;20070412;第8卷(第5期);第1607-1614页 * |
| The effect of VEGF functionalization of titanium on endothelial cells in vitro;Chye Khoon Poh et al.;《Biomaterials》;20091205;第31卷(第7期);第1578-1585页 * |
| VirendraSinghetal..Graphemebasedmaterials:past present and future.《Progress in Materials Science》.2011 |
| Wong Cheng Lee et al..Origin of Enhanced Stem Cell Growth and Differentiation on Graphene and Graphene Oxide.《ACS Nano》.2011,第5卷(第9期),第7334-7341页. |
| 徐东等.羧基化氧化石墨烯的血液相容性.《高等学校化学学报》.2010,第31卷(第12期),第2354-2359页. |
| 羧基化氧化石墨烯的血液相容性;徐东等;《高等学校化学学报》;20101231;第31卷(第12期);第2354-2359页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103100114A (en) | 2013-05-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103100114B (en) | Preparation method of medical metal surface slow-released growth factor coating | |
| CN103191467B (en) | Preparation method for antibacterial coat for fixing various cell growth factors on medical metal | |
| Eivazzadeh‐Keihan et al. | Metal‐based nanoparticles for bone tissue engineering | |
| Gao et al. | Polydopamine-templated hydroxyapatite reinforced polycaprolactone composite nanofibers with enhanced cytocompatibility and osteogenesis for bone tissue engineering | |
| Bidarra et al. | Immobilization of human mesenchymal stem cells within RGD-grafted alginate microspheres and assessment of their angiogenic potential | |
| Cheng et al. | Mussel-inspired multifunctional hydrogel coating for prevention of infections and enhanced osteogenesis | |
| CN103203039B (en) | Preparation method of coating with bone induction and antibiosis functions on surface of medical metal | |
| Xie et al. | Biomimetic mineralized hierarchical graphene oxide/chitosan scaffolds with adsorbability for immobilization of nanoparticles for biomedical applications | |
| CN102950102B (en) | Preparation method of multi-growth-factor slow release coating of titanium and titanium alloy surface | |
| Li et al. | Advances in the application of gold nanoparticles in bone tissue engineering | |
| Ko et al. | Polydopamine-assisted osteoinductive peptide immobilization of polymer scaffolds for enhanced bone regeneration by human adipose-derived stem cells | |
| Shi et al. | Therapeutic potential of chitosan and its derivatives in regenerative medicine | |
| Dong et al. | Multifunctional surface with enhanced angiogenesis for improving long-term osteogenic fixation of poly (ether ether ketone) implants | |
| Liu et al. | Off-the-shelf biomimetic graphene oxide–collagen hybrid scaffolds wrapped with osteoinductive extracellular matrix for the repair of cranial defects in rats | |
| Yan et al. | Applications of polydopamine-modified scaffolds in the peripheral nerve tissue engineering | |
| CN107949598A (en) | The honeycomb porous material for preparing the method for the hydrogel based on chitosan and negative polyelectrolytes and being obtained by the hydrogel | |
| Wang et al. | BMP-functionalised coatings to promote osteogenesis for orthopaedic implants | |
| Mirza et al. | Bioactive gum Arabic/κ-carrageenan-incorporated nano-hydroxyapatite nanocomposites and their relative biological functionalities in bone tissue engineering | |
| Sehgal et al. | Mechanically stiff, zinc cross-linked nanocomposite scaffolds with improved osteostimulation and antibacterial properties | |
| CN105327406A (en) | Method for preparing multi-layer heparin-carrying reduced graphene oxide coating | |
| Cheng et al. | Electrodeposited assembly of additive-free silk fibroin coating from pre-assembled nanospheres for drug delivery | |
| Guo et al. | Advanced Hydrogel systems for mandibular reconstruction | |
| CN107854726A (en) | A kind of compound rest and its preparation method and application | |
| Bozorgi et al. | Application of nanoparticles in bone tissue engineering; a review on the molecular mechanisms driving osteogenesis | |
| Wu et al. | Cryogel biocomposite containing chitosan-gelatin/cerium–zinc doped hydroxyapatite for bone tissue engineering |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140521 Termination date: 20180123 |